{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:13:08Z","timestamp":1790446388259,"version":"4.1.0"},"reference-count":31,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPESP","award":["2022\/15151-3"],"award-info":[{"award-number":["2022\/15151-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Polyploid Giant Cancer Cells (PGCCs) have been recognized as tumor cells that are resistant to anticancer therapies. However, it remains unclear whether their presence in the bloodstream can be consistently detected and utilized as a clinical marker to guide therapeutic anticancer regimens. To address these questions, we conducted a retrospective study involving 228 patients diagnosed with six different types of carcinomas (colon, gastric, NSCLC, breast, anal canal, kidney), with the majority of them (70%) being non-metastatic. Employing a highly sensitive liquid biopsy approach, ISET\u00ae, and cytopathological readout, we isolated and detected circulating PGCCs in the patients\u2019 blood samples. PGCCs were identified in 46 (20.18%) out of 228 patients, including in 14.47% of 152 non-metastatic and 29.85% of 67 metastatic cases. Patients were subsequently monitored for a mean follow up period of 44.74 months (95%CI: 33.39\u201355.79 months). Remarkably, the presence of circulating PGCCs emerged as a statistically significant indicator of poor overall survival. Our findings suggest that circulating PGCCs hold promise as a reliable prognostic indicator. They underscore the importance of further extensive investigations into the role of circulating PGCCs as a prognostic marker and the development of anti-PGCC therapeutic strategies to improve cancer management and patient survival.<\/jats:p>","DOI":"10.3390\/ijms25189841","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T02:30:23Z","timestamp":1726108223000},"page":"9841","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Circulating Polyploid Giant Cancer Cells, a Potential Prognostic Marker in Patients with Carcinoma"],"prefix":"10.3390","volume":"25","author":[{"given":"Ludmilla Thom\u00e9 Domingos","family":"Chinen","sequence":"first","affiliation":[{"name":"Hcor Research Institute, S\u00e3o Paulo 04004-030, Brazil"},{"name":"Hospital Amaral Carvalho, Ja\u00fa 17210-080, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0253-3479","authenticated-orcid":false,"given":"Jacqueline Aparecida","family":"Torres","sequence":"additional","affiliation":[{"name":"Department of Farmacology, Federal University of S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04044-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2332-5863","authenticated-orcid":false,"given":"Vinicius Fernando","family":"Calsavara","sequence":"additional","affiliation":[{"name":"Department of Computational Biomedicine, Biostatistics Shared Resource, Cedars-Sinai Cancer Center, Los Angeles, CA 90069, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo Borsarelli Carvalho","family":"Brito","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, A.C. Camargo Cancer Center, S\u00e3o Paulo 01509-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virg\u00edlio Sousa e","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, A.C. Camargo Cancer Center, S\u00e3o Paulo 01509-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Gabriel Santiago","family":"Novello","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, A.C. Camargo Cancer Center, S\u00e3o Paulo 01509-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaissa Carvalho","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, A.C. Camargo Cancer Center, S\u00e3o Paulo 01509-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Decina","sequence":"additional","affiliation":[{"name":"Rarecells Facult\u00e9 de M\u00e9decine Necker, 160 Rue de Vaugirard, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Dachez","sequence":"additional","affiliation":[{"name":"Cytopathology Laboratory Innodiag, F-92100 Boulogne-Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7981-6325","authenticated-orcid":false,"given":"Patrizia","family":"Paterlini-Brechot","sequence":"additional","affiliation":[{"name":"Rarecells Facult\u00e9 de M\u00e9decine Necker, 160 Rue de Vaugirard, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1111\/eva.12929","article-title":"Poly-aneuploid Cancer Cells Promote Evolvability, Generating Lethal Cancer","volume":"13","author":"Pienta","year":"2020","journal-title":"Evol. 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