{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T21:33:50Z","timestamp":1771364030124,"version":"3.50.1"},"reference-count":92,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["100382963"],"award-info":[{"award-number":["100382963"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To identify potentially transplantable organs in a pool of marginal kidneys, 33 porcine slaughterhouse kidneys were perfused for 4 h with whole blood. During the normothermic perfusion, plasma, urine, and tissue samples were taken. Several biomarkers for tubule injury, endothelial activation, and inflammatory response were evaluated for a potential correlation with macroscopic appearance, histology, and filtration activity. Generally, biomarker levels increased during perfusion. TLR-4, EDN-1, and NGAL were not associated with any classification. In contrast, a steeper increase in NAG and IL-6 in plasma correlated with a poor macroscopic appearance at 4 h, indicating a higher inflammatory response in the kidneys with worse macroscopy early on, potentially due to more damage at the tubules. Although long-term effects on the graft could not be assessed in this setting, early observation under machine perfusion with whole blood was feasible. It allowed the assessment of kidneys under conditions comparable to reperfusion. This setting could give surgeons further insight into the quality of marginal kidneys and an opportunity to pre-treat them.<\/jats:p>","DOI":"10.3390\/ijms251910280","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T10:41:47Z","timestamp":1727174507000},"page":"10280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessing Biomarkers of Porcine Kidneys under Normothermic Machine Perfusion\u2014Can We Gain Insight into a Marginal Organ?"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9011-0822","authenticated-orcid":false,"given":"Carla","family":"Steinhauser","sequence":"first","affiliation":[{"name":"Department of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulbaki","family":"Yakac","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0090-8265","authenticated-orcid":false,"given":"Wenke","family":"Markgraf","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Kromnik","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8786-5338","authenticated-orcid":false,"given":"Andreas","family":"D\u00f6cke","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Talhofer","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Thiele","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hagen","family":"Malberg","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Sommer","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo B.","family":"Baretton","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5339-9460","authenticated-orcid":false,"given":"Susanne","family":"F\u00fcssel","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliane","family":"Putz","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, D-01309 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1681\/ASN.V123589","article-title":"Survival in recipients of marginal cadaveric donor kidneys compared with other recipients and wait-listed transplant candidates","volume":"12","author":"Ojo","year":"2001","journal-title":"J. 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