{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T06:42:42Z","timestamp":1771051362495,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T00:00:00Z","timestamp":1727568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Direcci\u00f3n de Investigaci\u00f3n, Universidad de La Frontera","award":["PP24-0001"],"award-info":[{"award-number":["PP24-0001"]}]},{"name":"Direcci\u00f3n de Investigaci\u00f3n, Universidad de La Frontera","award":["21232282"],"award-info":[{"award-number":["21232282"]}]},{"name":"Scholarship Program\/Beca Doctorado Nacional\/2023","award":["PP24-0001"],"award-info":[{"award-number":["PP24-0001"]}]},{"name":"Scholarship Program\/Beca Doctorado Nacional\/2023","award":["21232282"],"award-info":[{"award-number":["21232282"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Corynebacterium striatum, present in the microbiota of human skin and nasal mucosa, has recently emerged as a causative agent of hospital-acquired infections, notable for its resistance to multiple antimicrobials. Its mobilome comprises several mobile genetic elements, such as plasmids, transposons, insertion sequences and integrons, which contribute to the acquisition of antimicrobial resistance genes. This study analyzes the contribution of the C. striatum mobilome in the transfer and dissemination of resistance genes. In addition, integrative and conjugative elements (ICEs), essential in the dissemination of resistance genes between bacterial populations, whose role in C. striatum has not yet been studied, are examined. This study examined 365 C. striatum genomes obtained from the NCBI Pathogen Detection database. Phylogenetic and pangenome analyses were performed, the resistance profile of the bacterium was recognized, and mobile elements, including putative ICE, were detected. Bioinformatic analyses identified 20 antimicrobial resistance genes in this species, with the Ermx gene being the most predominant. Resistance genes were mainly associated with plasmid sequence regions and class 1 integrons. Although an ICE was detected, no resistance genes linked to this element were found. This study provided valuable information on the geographic spread and prevalence of outbreaks observed through phylogenetic and pangenome analyses, along with identifying antimicrobial resistance genes and mobile genetic elements that carry many of the resistance genes and may be the subject of future research and therapeutic approaches.<\/jats:p>","DOI":"10.3390\/ijms251910499","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T03:12:28Z","timestamp":1727665948000},"page":"10499","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Contribution of the Mobilome to the Configuration of the Resistome of Corynebacterium striatum"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0206-5209","authenticated-orcid":false,"given":"Catherine","family":"Urrutia","sequence":"first","affiliation":[{"name":"Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Carrera de Biotecnolog\u00eda, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6362-3529","authenticated-orcid":false,"given":"Benjamin","family":"Leyton-Carcaman","sequence":"additional","affiliation":[{"name":"Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Doctorado en Ciencias Menci\u00f3n Biolog\u00eda Celular y Molecular Aplicada, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9136-0041","authenticated-orcid":false,"given":"Michel","family":"Abanto Marin","sequence":"additional","affiliation":[{"name":"Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Orosz, L., Lengyel, G., Makai, K., and Buri\u00e1n, K. (2023). Prescription of Rifampicin for Staphylococcus Aureus Infections Increased the Incidence of Corynebacterium Striatum with Decreased Susceptibility to Rifampicin in a Hungarian Clinical Center. Pathogens, 12.","DOI":"10.3390\/pathogens12030481"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2147\/IDR.S184518","article-title":"Molecular Epidemiology and Clinical Significance of Corynebacterium Striatum Isolated from Clinical Specimens","volume":"12","author":"Suh","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Orosz, L., S\u00f3ki, J., K\u00f3kai, D., and Buri\u00e1n, K. (2022). Corynebacterium Striatum\u2014Got Worse by a Pandemic?. Pathogens, 11.","DOI":"10.3390\/pathogens11060685"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1007\/s10482-019-01265-9","article-title":"Virulence Potential of Corynebacterium Striatum towards Caenorhabditis Elegans","volume":"112","author":"Mota","year":"2019","journal-title":"Antonie Leeuwenhoek Int. J. Gen. Mol. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1007\/s00203-021-02246-1","article-title":"Worldwide Survey of Corynebacterium Striatum Increasingly Associated with Human Invasive Infections, Nosocomial Outbreak, and Antimicrobial Multidrug-Resistance, 1976\u20132020","volume":"203","author":"Silva","year":"2021","journal-title":"Arch. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.chom.2019.03.006","article-title":"Strain- and Species-Level Variation in the Microbiome of Diabetic Wounds Is Associated with Clinical Outcomes and Therapeutic Efficacy","volume":"25","author":"Kalan","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_7","first-page":"15","article-title":"Risk Factors Associated with Osteomyelitis Due to Corynebacterium Striatum in Patients with Diabetic Foot","volume":"162","year":"2024","journal-title":"Med. Clin."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.3201\/eid2211.160141","article-title":"Multidrug-Resistant Corynebacterium Striatum Associated with Increased Use of Parenteral Antimicrobial Drugs","volume":"22","author":"Hahn","year":"2016","journal-title":"Emerg Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Leyton, B., Ramos, J.N., Baio, P.V.P., Veras, J.F.C., Souza, C., Burkovski, A., Mattos-Guaraldi, A.L., Vieira, V.V., and Abanto Marin, M. (2021). Treat Me Well or Will Resist: Uptake of Mobile Genetic Elements Determine the Resistome of Corynebacterium Striatum. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147499"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Yang, Y., Kuang, X., Han, R., Zhai, Y., He, D., Zhao, J., Liu, J., and Hu, G. (2022). Characterization of a Novel Linezolid Resistance Gene OptrA and Bacitracin Resistance Locus-Carrying Multiple Antibiotic Resistant Integrative and Conjugative Element ICE Ssu 1112S in Streptococccus Suis. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.01963-21"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Qiu, J., Shi, Y., Zhao, F., Xu, Y., Xu, H., Dai, Y., and Cao, Y. (2023). The Pan-Genomic Analysis of Corynebacterium Striatum Revealed Its Genetic Characteristics as an Emerging Multidrug-Resistant Pathogen. Evol. Bioinform., 19.","DOI":"10.1177\/11769343231191481"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e180051","DOI":"10.1590\/0074-02760180051","article-title":"Genome Sequence of a Multidrug-Resistant Corynebacterium Striatum Isolated from Bloodstream Infection from a Nosocomial Outbreak in Rio de Janeiro, Brazil","volume":"113","author":"Ramos","year":"2018","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e01776-17","DOI":"10.1128\/AAC.01776-17","article-title":"In Vitro Antibiotic Susceptibility Pattern of Non-Diphtheriae Corynebacterium Isolates in Ontario, Canada, from 2011 to 2016","volume":"62","author":"Neemuchwala","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Schr\u00f6der, J., Maus, I., Meyer, K., W\u00f6rdemann, S., Blom, J., Jaenicke, S., Schneider, J., Trost, E., and Tauch, A. (2012). Complete Genome Sequence, Lifestyle, and Multi-Drug Resistance of the Human Pathogen Corynebacterium Resistens DSM 45100 Isolated from Blood Samples of a Leukemia Patient. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-141"},{"key":"ref_15","first-page":"136","article-title":"Integron and Its Role in Antimicrobial Resistance: A Literature Review on Some Bacterial Pathogens","volume":"24","author":"Sabbagh","year":"2021","journal-title":"Iran J. Basic. Med. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kang, Y., Chen, S., Zheng, B., Du, X., Li, Z., Tan, Z., Zhou, H., Huang, J., Tian, L., and Zhong, J. (2023). Epidemiological Investigation of Hospital Transmission of Corynebacterium Striatum Infection by Core Genome Multilocus Sequence Typing Approach. Microbiol. Spectr., 11.","DOI":"10.1128\/spectrum.01490-22"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1590\/S0074-02762013000100004","article-title":"Clonal Multidrug-Resistant Corynebacterium Striatum within a Nosocomial Environment, Rio de Janeiro, Brazil","volume":"108","author":"Hirata","year":"2013","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00683-19","DOI":"10.1128\/JCM.00683-19","article-title":"Whole-Genome Sequencing Reveals a Prolonged and Persistent Intrahospital Transmission of Corynebacterium Striatum, an Emerging Multidrug-Resistant Pathogen","volume":"57","author":"Wang","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wang, J., Zhang, M., Pei, J., Yi, W., Fan, L., Wang, C., and Xiao, X. (2024). Isolation and Identification of a Novel Phage Targeting Clinical Multidrug-Resistant Corynebacterium Striatum Isolates. Front. Cell Infect. Microbiol., 14.","DOI":"10.3389\/fcimb.2024.1361045"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nageeb, W.M., and Hetta, H.F. (2023). Pangenome Analysis of Corynebacterium Striatum: Insights into a Neglected Multidrug-Resistant Pathogen. BMC Microbiol., 23.","DOI":"10.1186\/s12866-023-02996-6"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1007\/s10482-023-01855-8","article-title":"Phylogenetic Analyses of Antimicrobial Resistant Corynebacterium Striatum Strains Isolated from a Nosocomial Outbreak in a Tertiary Hospital in China","volume":"116","author":"Li","year":"2023","journal-title":"Antonie Leeuwenhoek Int. J. Gen. Mol. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jhin.2020.10.005","article-title":"Genomic Epidemiology of Corynebacterium Striatum from Three Regions of China: An Emerging National Nosocomial Epidemic","volume":"110","author":"Wang","year":"2021","journal-title":"J. Hosp. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e49","DOI":"10.1590\/s1678-9946202163049","article-title":"Antimicrobial Treatment of Corynebacterium Striatum Invasive Infections: A Systematic Review","volume":"63","author":"Milosavljevic","year":"2021","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Leyton-Carcaman, B., and Abanto, M. (2022). Beyond to the Stable: Role of the Insertion Sequences as Epidemiological Descriptors in Corynebacterium Striatum. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.806576"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bhat, B.A., Mir, R.A., Qadri, H., Dhiman, R., Almilaibary, A., Alkhanani, M., and Mir, M.A. (2023). Integrons in the Development of Antimicrobial Resistance: Critical Review and Perspectives. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1231938"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10142-022-00932-x","article-title":"The Pan-Genome of the Emerging Multidrug-Resistant Pathogen Corynebacterium Striatum","volume":"23","author":"Jesus","year":"2023","journal-title":"Funct. Integr. Genom."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.resmic.2004.01.012","article-title":"Shaping Bacterial Genomes with Integrative and Conjugative Elements","volume":"155","author":"Burrus","year":"2004","journal-title":"Res. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/nrmicro2382","article-title":"Integrative and Conjugative Elements: Mosaic Mobile Genetic Elements Enabling Dynamic Lateral Gene Flow","volume":"8","author":"Wozniak","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hirose, J. (2023). Diversity and Evolution of Integrative and Conjugative Elements Involved in Bacterial Aromatic Compound Degradation and Their Utility in Environmental Remediation. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020438"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1111\/mmi.14359","article-title":"Identification, Characterization and Benefits of an Exclusion System in an Integrative and Conjugative Element of Bacillus Subtilis","volume":"112","author":"Avello","year":"2019","journal-title":"Mol. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Johnson, C.M., Harden, M.M., and Grossman, A.D. (2022). Interactions between Mobile Genetic Elements: An Anti-Phage Gene in an Integrative and Conjugative Element Protects Host Cells from Predation by a Temperate Bacteriophage. PLoS Genet., 18.","DOI":"10.1371\/journal.pgen.1010065"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving Bacterial Genome Assemblies from Short and Long Sequencing Reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_34","unstructured":"(2024, July 20). Simon Andrews Babraham Bioinformatics-FastQC a Quality Control Tool for High Throughput Sequence Data. Available online: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Treangen, T.J., Ondov, B.D., Koren, S., and Phillippy, A.M. (2014). The Harvest Suite for Rapid Core-Genome Alignment and Visualization of Thousands of Intraspecific Microbial Genomes. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0524-x"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies","volume":"32","author":"Nguyen","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"93","DOI":"10.12688\/wellcomeopenres.14694.1","article-title":"RhierBAPS: An R Implementation of the Population Clustering Algorithm HierBAPS","volume":"3","author":"Lees","year":"2018","journal-title":"Wellcome Open Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1093\/molbev\/mst028","article-title":"Hierarchical and Spatially Explicit Clustering of DNA Sequences with BAPS Software","volume":"30","author":"Cheng","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid Prokaryotic Genome Annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: Rapid Large-Scale Prokaryote Pan Genome Analysis","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1093\/femsre\/fux008","article-title":"The Hidden Life of Integrative and Conjugative Elements","volume":"41","author":"Delavat","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40168-020-00867-0","article-title":"VIBRANT: Automated Recovery, Annotation and Curation of Microbial Viruses, and Evaluation of Viral Community Function from Genomic Sequences","volume":"8","author":"Kieft","year":"2020","journal-title":"Microbiome"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1093\/bioinformatics\/bts565","article-title":"CD-HIT: Accelerated for Clustering the next-Generation Sequencing Data","volume":"28","author":"Fu","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1093\/bioinformatics\/btx157","article-title":"ViPTree: The Viral Proteomic Tree Server","volume":"33","author":"Nishimura","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"D660","DOI":"10.1093\/nar\/gky1123","article-title":"ICEberg 2.0: An Updated Database of Bacterial Integrative and Conjugative Elements","volume":"47","author":"Liu","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: In-Depth Characterization and Visualization of Bacterial Genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gki408","article-title":"The HHpred Interactive Server for Protein Homology Detection and Structure Prediction","volume":"33","author":"Biegert","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_48","unstructured":"(2024, July 20). Raivo Kolde CRAN: Package Pheatmap. Available online: https:\/\/cran.r-project.org\/web\/packages\/pheatmap\/index.html."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (ITOL) v5: An Online Tool for Phylogenetic Tree Display and Annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1093\/bioinformatics\/bts091","article-title":"Unipro UGENE: A Unified Bioinformatics Toolkit","volume":"28","author":"Okonechnikov","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Branger, M., Ebastien, S., and Leclercq, O. (2024). GenoFig: A User-Friendly Application for the Visualization and Comparison of Genomic Regions. Bioinformatics, 40.","DOI":"10.1093\/bioinformatics\/btae372"},{"key":"ref_52","first-page":"685","article-title":"Bakta: Rapid and Standardized Annotation of Bacterial Genomes via Alignment-Free Sequence Identification","volume":"7","author":"Schwengers","year":"2021","journal-title":"Microb. Genom."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Panunzi, L.G. (2020). SraX: A Novel Comprehensive Resistome Analysis Tool. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00052"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and Applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e332","DOI":"10.7717\/peerj.332","article-title":"The Large-Scale Blast Score Ratio (LS-BSR) Pipeline: A Method to Rapidly Compare Genetic Content between Bacterial Genomes","volume":"2014","author":"Sahl","year":"2014","journal-title":"PeerJ"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"D195","DOI":"10.1093\/nar\/gky1050","article-title":"PLSDB: A Resource of Complete Bacterial Plasmids","volume":"47","author":"Galata","year":"2019","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/19\/10499\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:06:38Z","timestamp":1760112398000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/19\/10499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,29]]},"references-count":56,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["ijms251910499"],"URL":"https:\/\/doi.org\/10.3390\/ijms251910499","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,29]]}}}