{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:50:28Z","timestamp":1785153028291,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Academic Scientist Fund for Zhongyuan Scholars of Henan Province","award":["2018 [99]"],"award-info":[{"award-number":["2018 [99]"]}]},{"name":"Academic Scientist Fund for Zhongyuan Scholars of Henan Province","award":["2023M730989"],"award-info":[{"award-number":["2023M730989"]}]},{"name":"Academic Scientist Fund for Zhongyuan Scholars of Henan Province","award":["HN2022129"],"award-info":[{"award-number":["HN2022129"]}]},{"name":"73rd batch of the China Postdoctoral Science Foundation","award":["2018 [99]"],"award-info":[{"award-number":["2018 [99]"]}]},{"name":"73rd batch of the China Postdoctoral Science Foundation","award":["2023M730989"],"award-info":[{"award-number":["2023M730989"]}]},{"name":"73rd batch of the China Postdoctoral Science Foundation","award":["HN2022129"],"award-info":[{"award-number":["HN2022129"]}]},{"name":"2022 Postdoctoral Research Grant from Henan Province","award":["2018 [99]"],"award-info":[{"award-number":["2018 [99]"]}]},{"name":"2022 Postdoctoral Research Grant from Henan Province","award":["2023M730989"],"award-info":[{"award-number":["2023M730989"]}]},{"name":"2022 Postdoctoral Research Grant from Henan Province","award":["HN2022129"],"award-info":[{"award-number":["HN2022129"]}]},{"name":"2023 Provincial Science and Technology Research and Development Program Joint Fund","award":["2018 [99]"],"award-info":[{"award-number":["2018 [99]"]}]},{"name":"2023 Provincial Science and Technology Research and Development Program Joint Fund","award":["2023M730989"],"award-info":[{"award-number":["2023M730989"]}]},{"name":"2023 Provincial Science and Technology Research and Development Program Joint Fund","award":["HN2022129"],"award-info":[{"award-number":["HN2022129"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Paulownia species not only have significant economic benefits but also show great potential in ecological conservation. However, they are highly susceptible to phytoplasma infections, causing Paulownia witches\u2019 broom (PaWB), which severely restricts the development of the Paulownia industry. Salicylic acid (SA) plays a crucial role in plant disease resistance. However, there have been no reports on the effect of SA on PaWB. Due to the properties of SA, it may have potential in controlling PaWB. Based on the above speculation, the prevention and therapeutic effect of SA on PaWB and its effect on the PaWB-infected Paulownia transcriptome and proteome were studied in this work. The results indicated that 0.1 mmol\/L was the optimal SA concentration for inhibiting the germination of Paulownia axillary buds. In terms of resistance physiological indicators, SA treatment significantly affected both Paulownia tomentosa infected (PTI) seedlings and Paulownia fortunei infected (PFI) seedlings, where the activities of peroxidase (POD) and superoxide dismutase (SOD) were enhanced. Malondialdehyde (MDA), O2\u2212, and H2O2, however, were significantly reduced. Specifically, after SA treatment, SOD activity increased by 28% in PFI and 25% in PTI, and POD activity significantly increased by 61% in PFI and 58% in PTI. Moreover, the MDA content decreased by 30% in PFI and 23% in PTI, the H2O2 content decreased by 26% in PFI and 19% in PTI, and the O2\u2212 content decreased by 21% in PFI and 19% in PTI. Transcriptomic analysis showed that there were significant upregulations of MYB, NAC, and bHLH and other transcription factors after SA treatment. Moreover, genes involved in PaWB-related defense responses such as RAX2 also showed significant differences. Furthermore, proteomic analysis indicated that after SA treatment, proteins involved in signal transduction, protein synthesis modification, and disease defense were differentially expressed. This work provides a research foundation for the prevention and treatment of PaWB and offers references for exploring anti-PaWB methods.<\/jats:p>","DOI":"10.3390\/ijms251910553","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T10:00:47Z","timestamp":1727690447000},"page":"10553","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Novel Insight into the Prevention and Therapeutic Treatment of Paulownia Witches\u2019 Broom: A Study on the Effect of Salicylic Acid on Disease Control and the Changes in the Paulownia Transcriptome and Proteome"],"prefix":"10.3390","volume":"25","author":[{"given":"Yujie","family":"Fan","sequence":"first","affiliation":[{"name":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China"},{"name":"Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China"},{"name":"Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China"},{"name":"Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China"},{"name":"Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China"},{"name":"Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China"},{"name":"Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jakubowski, M. 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