{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:27:13Z","timestamp":1760146033981,"version":"build-2065373602"},"reference-count":71,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Croatian National Science Foundation Project PREDI-COO","award":["IP-2019-04-9308"],"award-info":[{"award-number":["IP-2019-04-9308"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer, ranking as the sixth most common cancer worldwide and the fourth leading cause of cancer-related deaths. Most HCC cases originate from cirrhotic livers, typically due to chronic liver diseases, such as hepatitis B (HBV) and hepatitis C (HCV) infections, and alcoholism. HCC cells often harbor numerous somatic mutations that are implicated in HCC development, but epigenetic factors, such as miRNA interference, can also affect HCC initiation and progress. miRNA-221 has been explored as a factor affecting HCC development in HCC of viral etiology, but little is known about its effects on gene expression in alcohol-associated HCC. This study aimed to explore potentially similar gene expression aberrations underlying viral and alcohol-induced HCC. We analyzed available transcriptome data from non-tumor hepatocytes and viral-induced HCC tissues. The most notable differences in gene expression associated with miRNA-221 between non-tumor hepatocytes and viral-induced HCC involved NTF-3 and MYBL1 genes. To assess these data in alcohol-induced HCC, we examined 111 tissue samples: tumor tissue and cirrhotic tissue samples from 37 HCC patients and 37 samples from non-tumor liver tissue using RT-Q PCR. We found no significant difference in NTF-3 expression, but MYBL1 expression was significantly lower in HCC tissue compared to non-tumor hepatocytes and cirrhotic tissue. Our findings highlight the importance of the MYBL1 gene in HCC development and emphasize the need for diverse approaches in evaluating tumor mechanisms.<\/jats:p>","DOI":"10.3390\/ijms251910558","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T11:32:05Z","timestamp":1727695925000},"page":"10558","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gene Expression Aberrations in Alcohol-Associated Hepatocellular Carcinoma"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0883-2763","authenticated-orcid":false,"given":"Andreja","family":"Petrovi\u0107","sequence":"first","affiliation":[{"name":"Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Institute of Clinical Pathology and Cytology, Merkur University Hospital, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"\u0160tancl","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5667-3843","authenticated-orcid":false,"given":"Paula","family":"Gr\u0161kovi\u0107","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7864-7996","authenticated-orcid":false,"given":"Suzana","family":"Han\u010di\u0107","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pathology and Cytology, Merkur University Hospital, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1291-2897","authenticated-orcid":false,"given":"Rosa","family":"Karli\u0107","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slavko","family":"Ga\u0161parov","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pathology and Cytology, Merkur University Hospital, 10000 Zagreb, Croatia"},{"name":"Department of Pathology, Medical School Zagreb, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4783-2114","authenticated-orcid":false,"given":"Petra","family":"Kora\u0107","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2019). Who Classification of Tumours: Digestive System Tumours, World Health Organization (WHO)."},{"key":"ref_2","unstructured":"Srivastava, A., Allende, D.S., and Goldblum, J.R. (2023). Gastrointestinal and Liver Pathology: A Volume in the Series: Foundations in Diagnostic Pathology, Elsevier."},{"key":"ref_3","unstructured":"Christopher, D.M.F. (2021). Diagnostic Histopathology of Tumors, Elsevier."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1093\/alcalc\/agz036","article-title":"Alcoholic Hepatitis: A Review","volume":"54","author":"Hosseini","year":"2019","journal-title":"Alcohol Alcohol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jare.2018.12.006","article-title":"Beneficial role of bioactive lipids in the pathobiology, prevention, and management of HBV, HCV and alcoholic hepatitis, NAFLD, and liver cirrhosis: A review","volume":"17","author":"Das","year":"2018","journal-title":"J. Adv. Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mohr, R., \u00d6zdirik, B., Lambrecht, J., Demir, M., Eschrich, J., Geisler, L., Hellberg, T., Loosen, S.H., Luedde, T., and Tacke, F. (2021). From Liver Cirrhosis to Cancer: The Role of Micro-RNAs in Hepatocarcinogenesis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031492"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Oura, K., Morishita, A., and Masaki, T. (2020). Molecular and Functional Roles of MicroRNAs in the Progression of Hepatocellular carcinoma\u2014A Review. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21218362"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1002\/jcp.26938","article-title":"MicroRNAs in alcoholic liver disease: Recent advances and future applications","volume":"234","author":"Xu","year":"2018","journal-title":"J. Cell Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Cucarull, B., Tutusaus, A., Rider, P., Hern\u00e1ez-Alsina, T., Cu\u00f1o, C., Garc\u00eda de Frutos, P., Colell, A., Mar\u00ed, M., and Morales, A. (2022). Hepatocellular carcinoma: Molecular Pathogenesis and Therapeutic Advances. Cancers, 14.","DOI":"10.3390\/cancers14030621"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1038\/s43018-022-00357-2","article-title":"Molecular pathogenesis and systemic therapies for Hepatocellular carcinoma","volume":"3","author":"Llovet","year":"2022","journal-title":"Nat. Cancer"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Patil, V.S., Harish, D.R., Sampat, G.H., Roy, S., Jalalpure, S.S., Khanal, P., Gujarathi, S.S., and Hegde, H.V. (2023). System Biology Investigation Revealed Lipopolysaccharide and Alcohol-Induced Hepatocellular carcinoma Resembled Hepatitis B Virus Immunobiology and Pathogenesis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241311146"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100167","DOI":"10.1016\/j.jhepr.2020.100167","article-title":"Epigenetics in Hepatocellular carcinoma development and therapy: The tip of the iceberg","volume":"2","author":"Arechederra","year":"2020","journal-title":"JHEP Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40169-019-0230-0","article-title":"Epigenetics of Hepatocellular carcinoma","volume":"8","author":"Toh","year":"2019","journal-title":"Clin. Transl. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Morishita, A., Oura, K., Tadokoro, T., Fujita, K., Tani, J., and Masaki, T. (2021). MicroRNAs in the Pathogenesis of Hepatocellular carcinoma: A Review. Cancers, 13.","DOI":"10.3390\/cancers13030514"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.3892\/or.2014.3306","article-title":"Bioinformatics analysis identifies miR-221 as a core regulator in Hepatocellular carcinoma and its silencing suppresses tumor properties","volume":"32","author":"He","year":"2014","journal-title":"Oncol. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2520","DOI":"10.7150\/jca.29207","article-title":"Clustered microRNAs hsa-miR-221-3p\/hsa-miR-222-3p and their targeted genes might be prognostic predictors for Hepatocellular carcinoma","volume":"10","author":"Wang","year":"2019","journal-title":"J. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.meegid.2017.03.030","article-title":"miRNA-101-1 and miRNA-221 expressions and their polymorphisms as biomarkers for early diagnosis of Hepatocellular carcinoma","volume":"51","author":"Shaker","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Loureiro, D., Tout, I., Narguet, S., Benazzouz, S.M., Mansouri, A., and Asselah, T. (2020). miRNAs as Potential Biomarkers for Viral Hepatitis B and C. Viruses, 12.","DOI":"10.3390\/v12121440"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"498232","DOI":"10.1155\/2012\/498232","article-title":"MicroRNA Signature in Alcoholic Liver Disease","volume":"2012","author":"Bala","year":"2012","journal-title":"Int. J. Hepatol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1073\/pnas.0907904107","article-title":"miR-221 overexpression contributes to liver tumorigenesis","volume":"107","author":"Pineau","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7252013","DOI":"10.1155\/2019\/7252013","article-title":"Role of miR-221\/222 in Tumor Development and the Underlying Mechanism","volume":"2019","author":"Song","year":"2019","journal-title":"J. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2147\/JHC.S156701","article-title":"Role of Wnt\/\u03b2-catenin signaling in Hepatocellular carcinoma, pathogenesis, and clinical significance","volume":"5","author":"Khalaf","year":"2018","journal-title":"J. Hepatocell. Carcinoma"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6035","DOI":"10.3748\/wjg.v16.i48.6035","article-title":"Oxidative stress and antioxidants in hepatic pathogenesis","volume":"16","author":"Ha","year":"2010","journal-title":"World J. Gastroenterol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Liu, R., Li, R., Yu, H., Liu, J., Zheng, S., Li, Y., and Ye, L. (2021). NTF3 Correlates With Prognosis and Immune Infiltration in Hepatocellular carcinoma. Front. Med., 8.","DOI":"10.3389\/fmed.2021.795849"},{"key":"ref_25","first-page":"141","article-title":"miRNA-222 promotes liver cancer cell proliferation, migration and invasion and inhibits apoptosis by targeting BBC3","volume":"42","author":"Liu","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9423907","DOI":"10.1155\/2019\/9423907","article-title":"The Role of Matrix Metalloproteinases in the Epithelial-Mesenchymal Transition of Hepatocellular carcinoma","volume":"2019","author":"Scheau","year":"2019","journal-title":"Anal. Cell. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/s41419-022-05180-2","article-title":"MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human Hepatocellular carcinoma","volume":"13","author":"Zhu","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_28","first-page":"4040787","article-title":"Plexin C1 Marks Liver Cancer Cells with Epithelial Phenotype and Is Overexpressed in Hepatocellular carcinoma","volume":"13","author":"Odabas","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_29","first-page":"1401","article-title":"SUN2: A potential therapeutic target in cancer","volume":"17","author":"Chen","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0079-6123(03)46016-0","article-title":"Neurotrophin-3 in the development of the enteric nervous system","volume":"146","author":"Chalazonitis","year":"2004","journal-title":"Prog. Brain Res."},{"key":"ref_31","first-page":"2280","article-title":"Low expression of NTF3 is associated with unfavorable prognosis in Hepatocellular carcinoma","volume":"13","author":"Yang","year":"2020","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.expneurol.2004.01.002","article-title":"Neurotrophin-3-induced PI-3 kinase\/Akt signaling rescues cortical neurons from apoptosis","volume":"187","author":"Liot","year":"2004","journal-title":"Exp. Neurol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Tan, F., Thiele, C.J., and Li, Z. (2014). Neurotrophin Signaling in Cancer. Handbook of Neurotoxicity, Springer.","DOI":"10.1007\/978-1-4614-5836-4_49"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/nrc2439","article-title":"MYB function in normal and cancer cells","volume":"8","author":"Ramsay","year":"2008","journal-title":"Nat. Rev. Cancer"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1038\/sj.onc.1202839","article-title":"The myb gene family in cell growth, differentiation and apoptosis","volume":"18","author":"Oh","year":"1999","journal-title":"Oncogene"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/bies.950170410","article-title":"MYB: An old oncoprotein with new roles","volume":"17","author":"Thompson","year":"1995","journal-title":"BioEssays"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1242\/dev.067645","article-title":"A-MYB (MYBL1) transcription factor is a master regulator of male meiosis","volume":"138","author":"Bannister","year":"2011","journal-title":"Development"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1042\/bj3240729","article-title":"Regulatory domains of the A-Myb transcription factor and its interaction with the CBP\/p300 adaptor molecules","volume":"324","author":"Facchinetti","year":"1997","journal-title":"Biochem. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"271","DOI":"10.3109\/10428199709051776","article-title":"The A-myb transcription factor in neoplastic and normal B cells","volume":"26","author":"Golay","year":"1997","journal-title":"Leuk. Lymphoma"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/s41419-019-1385-8","article-title":"Neurotrophins and their involvement in digestive cancers","volume":"10","author":"Blondy","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5963","DOI":"10.7150\/ijbs.72982","article-title":"Neurotrophin3 promotes Hepatocellular carcinoma apoptosis through the JNK and P38 MAPK pathways","volume":"18","author":"Yang","year":"2022","journal-title":"Int. J. Biol. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Guo, Y., Hu, J., Zhao, Z., Zhong, G., Gong, J., and Cai, D. (2021). Identification of a Prognostic Model Based on 2-Gene Signature and Analysis of Corresponding Tumor Microenvironment in Alcohol-Related Hepatocellular carcinoma. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.719355"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e6375","DOI":"10.7717\/peerj.6375","article-title":"Identification of special key genes for alcohol-related Hepatocellular carcinoma through bioinformatic analysis","volume":"7","author":"Zhang","year":"2019","journal-title":"PeerJ"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1038\/onc.2012.417","article-title":"Neurotrophin-3 modulates breast cancer cells and the microenvironment to promote the growth of breast cancer brain metastasis","volume":"32","author":"Louie","year":"2013","journal-title":"Oncogene"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/(SICI)1096-9896(199704)181:4<405::AID-PATH786>3.0.CO;2-3","article-title":"Neurotrophin-3 expression in human pancreatic cancers","volume":"181","author":"Ohta","year":"1997","journal-title":"J. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1165\/ajrcmb.25.4.4470","article-title":"Neurotrophins and neurotrophin receptors in human lung cancer","volume":"25","author":"Ricci","year":"2001","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1586\/ers.10.29","article-title":"Neurotrophins in lung health and disease","volume":"4","author":"Prakash","year":"2010","journal-title":"Expert Rev. Respir. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1172\/JCI41013","article-title":"Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis","volume":"120","author":"Cabrera","year":"2010","journal-title":"J. Clin. Investig."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1038\/sj.onc.1201282","article-title":"Linking Myb to the cell cycle: Cyclin-dependent phosphorylation and regulation of A-Myb activity","volume":"15","author":"Ziebold","year":"1997","journal-title":"Oncogene"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.omto.2020.05.016","article-title":"MYB Proto-oncogene-like 1-TWIST1 Axis Promotes Growth and Metastasis of Hepatocellular carcinoma Cells","volume":"18","author":"Xie","year":"2020","journal-title":"Mol. Ther. Oncolytics"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Player, A., Cunningham, S., Philio, D., Roy, R., Haynes, C., Dixon, C., Thirston, L., Ibikunle, F., Boswell, T.A., and Alnakhalah, A. (2024). Characterization of MYBL1 Gene in Triple-Negative Breast Cancers and the Genes\u2019 Relationship to Alterations Identified at the Chromosome 8q Loci. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25052539"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1158\/2159-8290.CD-15-0859","article-title":"Recurrent Fusions in MYB and MYBL1 Define a Common, Transcription Factor-Driven Oncogenic Pathway in Salivary Gland Adenoid cystic carcinoma","volume":"6","author":"Brayer","year":"2016","journal-title":"Cancer Discov."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8188","DOI":"10.1073\/pnas.1300252110","article-title":"Genomic analysis of diffuse pediatric low-grade gliomas identifies recurrent oncogenic truncating rearrangements in the transcription factor MYBL1","volume":"110","author":"Ramkissoon","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Persson, M., Andersson, M.K., Mitani, Y., Brandwein-Weber, M.S., Frierson, H.F., Moskaluk, C., Fonseca, I., Ferrarotto, R., Boecker, W., and Loening, T. (2022). Rearrangements, Expression, and Clinical Significance of MYB and MYBL1 in Adenoid cystic carcinoma: A Multi-Institutional Study. Cancers, 14.","DOI":"10.3390\/cancers14153691"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Gorbatenko, A., S\u00f8kilde, R., Sorensen, E.E., Newie, I., Persson, H., Morancho, B., Arribas, J., Litman, T., Rovira, C., and Pedersen, S.F. (2019). HER2 and p95HER2 differentially regulate miRNA expression in MCF-7 breast cancer cells and downregulate MYB proteins through miR-221\/222 and miR-503. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-39733-x"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1016\/j.ejca.2005.08.004","article-title":"B-MYB, a transcription factor implicated in regulating cell cycle, apoptosis and cancer","volume":"41","author":"Sala","year":"2005","journal-title":"Eur. J. Cancer"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2732","DOI":"10.1101\/gad.10.21.2732","article-title":"A dominant interfering Myb mutant causes apoptosis in T cells","volume":"10","author":"Taylor","year":"1996","journal-title":"Genes Dev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s10014-017-0282-z","article-title":"A comprehensive review of paediatric low-grade diffuse glioma: Pathology, molecular genetics and treatment","volume":"34","author":"Ryall","year":"2017","journal-title":"Brain Tumor Pathol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s10014-014-0200-6","article-title":"Molecular genetics of ependymomas and pediatric diffuse gliomas: A short review","volume":"31","author":"Nobusawa","year":"2014","journal-title":"Brain Tumor Pathol."},{"key":"ref_60","first-page":"1991","article-title":"Apoptotic response to oncogenic stimuli: Cooperative and antagonistic interactions between c-myb and the growth suppressor p53","volume":"56","author":"Sala","year":"1996","journal-title":"Cancer Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1007\/s10147-012-0431-7","article-title":"Immunohistochemical study of PUMA, c-Myb and p53 expression in the benign and malignant lesions of gallbladder and their clinicopathological significances","volume":"18","author":"Shu","year":"2012","journal-title":"Int. J. Clin. Oncol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e71","DOI":"10.1093\/nar\/gkv1507","article-title":"TCGAbiolinks: An R\/Bioconductor package for integrative analysis of TCGA data","volume":"44","author":"Colaprico","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_63","unstructured":"Cancer Genome Atlas Research Network (2017). Comprehensive and Integrative Genomic Characterization of Hepatocellular carcinoma. Cell, 169, 1327\u20131341.e23."},{"key":"ref_64","unstructured":"Babraham Bioinformatics (2023, July 10). FastQC: A Quality Control Tool for High Throughput Sequence Data 2023. Available online: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: An efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_69","unstructured":"Stephens, M., Carbonetto, P., Gerard, D., Lu, M., Sun, L., Willwerscheid, J., and Xiao, N. (2024, July 10). ashr: Methods for Adaptive Shrinkage, Using Empirical Bayes. R Package Version 2.2-54. Available online: https:\/\/CRAN.R-project.org\/package=ashr."},{"key":"ref_70","unstructured":"R Core Team (2024). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.omtm.2020.12.010","article-title":"Human-specific GAPDH qRT-PCR is an accurate and sensitive method of xenograft metastasis quantification","volume":"20","author":"Dahn","year":"2020","journal-title":"Mol. Ther.-Methods Clin. Dev."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/19\/10558\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:07:59Z","timestamp":1760112479000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/19\/10558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,30]]},"references-count":71,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["ijms251910558"],"URL":"https:\/\/doi.org\/10.3390\/ijms251910558","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,9,30]]}}}