{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:57:19Z","timestamp":1769507839636,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Vision Research"},{"name":"University of Melbourne Annemarie Mankiewicz-Zelkin Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Post-operative fibrosis of the filtering bleb limits the success of glaucoma filtration surgery (GFS). To minimise subconjunctival scarring following GFS, treatment with antimetabolites such as Mitomycin C (MMC) has become standard practice; however, their use is associated with considerable side effects. This study aimed to investigate the anti-scarring properties of 3\u2032,4\u2032-dihydroxyflavonol (DiOHF). GFS was performed in New Zealand white rabbits who received eye drops of DiOHF three times daily and vehicle eye drops after surgery (n = 5) or a single intraoperative treatment of MMC (n = 5). Blebs were imaged immediately following surgery and on days 7, 15, 21, and 28 for clinical examination. On day 28, eyes were harvested to assess collagen deposition, expression of \u03b1-SMA, oxidative stress, angiogenesis, fibroblast activity, and inflammation in the conjunctiva\/Tenon\u2019s layer. At 7 and 28 days post-GFS, MMC-treated blebs were more ischaemic than DiOHF- or vehicle-treated blebs. On day 28, DiOHF treatment significantly suppressed collagen accumulation, CD31 expression, Vimentin expression, and CD45 expression compared to the vehicle control. No difference was observed in 3-Nitrotyrosine or \u03b1SMA expression between treatment groups. Treatment with DiOHF reduced conjunctival scarring and angiogenesis in rabbits with GFS, which was comparable to MMC. DiOHF may be a safer and more effective wound-modulating agent than conventional antifibrotic therapy in GFS.<\/jats:p>","DOI":"10.3390\/ijms251910767","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T06:23:18Z","timestamp":1728282198000},"page":"10767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["3\u2032,4\u2032-Dihydroxyflavonol Inhibits Fibrotic Response in a Rabbit Model of Glaucoma Filtration Surgery"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1162-5378","authenticated-orcid":false,"given":"Zoe","family":"Pasvanis","sequence":"first","affiliation":[{"name":"Ophthalmology, Department of Surgery, University of Melbourne, Fitzroy, VIC 3065, Australia"},{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy C. K.","family":"Kong","sequence":"additional","affiliation":[{"name":"Ophthalmology, Department of Surgery, University of Melbourne, Fitzroy, VIC 3065, Australia"},{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manisha H.","family":"Shah","sequence":"additional","affiliation":[{"name":"Ophthalmology, Department of Surgery, University of Melbourne, Fitzroy, VIC 3065, Australia"},{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6604-5426","authenticated-orcid":false,"given":"Elsa C.","family":"Chan","sequence":"additional","affiliation":[{"name":"Ophthalmology, Department of Surgery, University of Melbourne, Fitzroy, VIC 3065, Australia"},{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia"},{"name":"Department of Medicine, St Vincent\u2019s Hospital, University of Melbourne, Fitzroy, VIC 3065, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer C.","family":"Fan Gaskin","sequence":"additional","affiliation":[{"name":"Ophthalmology, Department of Surgery, University of Melbourne, Fitzroy, VIC 3065, Australia"},{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia"},{"name":"Glaucoma Research and Investigation Unit, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"ref_1","unstructured":"Glaucoma Australia (2024, March 03). \u2018Silent Thief of Sight\u2019 Threatens to Rob Economy of Billions. 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