{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:40:20Z","timestamp":1786466420247,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Multiple system atrophy and Lewy body diseases (LBDs) such as Parkinson\u2019s disease, dementia with Lewy bodies, and Parkinson\u2019s disease with dementia, known as synucleinopathies, are defined neuropathologically by the accumulation and deposition of aberrant protein aggregates, primarily in neuronal cells. Seeding aggregation assays (SAA) have significant potential as biomarkers for early diagnosis, monitoring disease progression, and evaluating treatment efficacy for these diseases. Real-time quaking-induced conversion (RT-QuIC) and Protein Misfolding Cyclic Amplification (PMCA) assays represent two ultrasensitive protein amplification techniques that were initially tested for the field of prion disorders. Although the fundamental idea behind the creation of these two methods is very similar, their technical differences resulted in different levels of diagnostic accuracy for the identification of prion proteins, making the RT-QuIC assay the most trustworthy and effective instrument for the detection of suspected cases of LBDs and prion-like diseases.<\/jats:p>","DOI":"10.3390\/ijms251910783","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T04:29:26Z","timestamp":1728361766000},"page":"10783","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Seeding Aggregation Assays in Lewy Bodies Disorders: A Narrative State-of-the-Art Review"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3006-8711","authenticated-orcid":false,"given":"Anastasia","family":"Bougea","sequence":"first","affiliation":[{"name":"1st Department of Neurology, \u201cAiginition\u201d Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Paciotti, S., Bellomo, G., Gatticchi, L., and Parnetti, L. (2018). Are We Ready for Detecting \u03b1-Synuclein Prone to Aggregation in Patients? The Case of \u201cProtein-Misfolding Cyclic Amplification\u201d and \u201cReal-Time Quaking-Induced Conversion\u201d as Diagnostic Tools. Front. Neurol., 9.","DOI":"10.3389\/fneur.2018.00415"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/bs.acc.2020.08.007","article-title":"Synuclein in neurodegeneration","volume":"103","author":"Bougea","year":"2021","journal-title":"Adv. Clin. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1136\/jnnp-2014-310256","article-title":"What do patients with scans without evidence of dopaminergic deficit (SWEDD) have? New evidence and continuing controversies","volume":"87","author":"Erro","year":"2016","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s13024-021-00501-z","article-title":"Neuropathology and molecular diagnosis of Synucleinopathies","volume":"16","author":"Koga","year":"2021","journal-title":"Mol. Neurodegener."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10286-022-00882-1","article-title":"Recent advances in establishing fluid biomarkers for the diagnosis and differentiation of alpha-synucleinopathies\u2014A mini review","volume":"32","author":"Singer","year":"2022","journal-title":"Clin. Auton. Res. Off. J. Clin. Auton. Res. Soc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1177\/0004867419835029","article-title":"Dementia with Lewy bodies: Challenges in the diagnosis and management","volume":"53","author":"Chin","year":"2019","journal-title":"Aust. N. Z. J. Psychiatry"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S1474-4422(21)00030-2","article-title":"Challenges in the diagnosis of Parkinson\u2019s disease","volume":"20","author":"Tolosa","year":"2021","journal-title":"Lancet Neurol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/bs.acc.2019.12.001","article-title":"New markers in Parkinson\u2019s disease","volume":"96","author":"Bougea","year":"2020","journal-title":"Adv. Clin. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.1002\/mds.28715","article-title":"Seed Amplification Assay to Diagnose Early Parkinson\u2019s and Predict Dopaminergic Deficit Progression","volume":"36","author":"Farris","year":"2021","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1002\/acn3.51753","article-title":"Central and peripheral \u03b1-synuclein in Parkinson disease detected by seed amplification assay","volume":"10","author":"Chahine","year":"2023","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nm.2294","article-title":"Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion","volume":"17","author":"Atarashi","year":"2011","journal-title":"Nat. Med."},{"key":"ref_12","first-page":"3298","article-title":"Sporadic Creutzfeldt-Jakob disease: Real-Time Quaking Induced Conversion (RT-QuIC) assay represents a major diagnostic advance","volume":"65","author":"Cazzaniga","year":"2021","journal-title":"Eur. J. Histochem. EJH"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Vascellari, S., Orr\u00f9, C.D., and Caughey, B. (2022). Real-Time Quaking- Induced Conversion Assays for Prion Diseases, Synucleinopathies, and Tauopathies. Front. Aging Neurosci., 14.","DOI":"10.3389\/fnagi.2022.853050"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S1474-4422(23)00109-6","article-title":"Assessment of heterogeneity among participants in the Parkinson\u2019s Progression Markers Initiative cohort using \u03b1-synuclein seed amplification: A cross-sectional study","volume":"22","author":"Siderowf","year":"2023","journal-title":"Lancet Neurol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1002\/acn3.338","article-title":"Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies","volume":"3","author":"Fairfoul","year":"2016","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1001\/jamaneurol.2016.4547","article-title":"Development of a Biochemical Diagnosis of Parkinson Disease by Detection of \u03b1-Synuclein Misfolded Aggregates in Cerebrospinal Fluid","volume":"74","author":"Shahnawaz","year":"2017","journal-title":"JAMA Neurol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40478-018-0508-2","article-title":"Rapid and ultra-sensitive quantitation of disease-associated \u03b1-synuclein seeds in brain and cerebrospinal fluid by \u03b1Syn RT-QuIC","volume":"6","author":"Groveman","year":"2018","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Li, J., Luo, H., Zheng, H., Duan, S., Zhao, T., Yuan, Y., Liu, Y., Zhang, X., Wang, Y., and Yang, J. (2022). Clinical application of prion-like seeding in \u03b1-synucleinopathies: Early and non-invasive diagnosis and therapeutic development. Front. Mol. Neurosci., 15.","DOI":"10.3389\/fnmol.2022.975619"},{"key":"ref_19","first-page":"3916","article-title":"Prion-Like Seeding of Misfolded \u03b1-Synuclein in the Brains of Dementia with Lewy Body Patients in RT-QUIC","volume":"55","author":"Sano","year":"2018","journal-title":"Mol. Neurobiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s11481-019-09835-4","article-title":"Ultrasensitive Detection of Aggregated \u03b1-Synuclein in Glial Cells, Human Cerebrospinal Fluid, and Brain Tissue Using the RT-QuIC Assay: New High-Throughput Neuroimmune Biomarker Assay for Parkinsonian Disorders","volume":"14","author":"Manne","year":"2019","journal-title":"J. Neuroimmune Pharmacol. Off. J. Soc. NeuroImmune Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1080\/19336896.2020.1724608","article-title":"RT-QuIC-based detection of alpha-synuclein seeding activity in brains of dementia with Lewy Body patients and of a transgenic mouse model of synucleinopathy","volume":"14","author":"Han","year":"2020","journal-title":"Prion"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/s41586-020-1984-7","article-title":"Discriminating \u03b1-synuclein strains in Parkinson\u2019s disease and multiple system atrophy","volume":"578","author":"Shahnawaz","year":"2020","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1002\/ana.25824","article-title":"Alpha-Synuclein Oligomers and Neurofilament Light Chain in Spinal Fluid Differentiate Multiple System Atrophy from Lewy Body Synucleinopathies","volume":"88","author":"Singer","year":"2020","journal-title":"Ann. Neurol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1002\/mds.27646","article-title":"Comparative study of cerebrospinal fluid \u03b1-synuclein seeding aggregation assays for diagnosis of Parkinson\u2019s disease","volume":"34","author":"Kang","year":"2019","journal-title":"Mov. Disord."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1002\/mds.29329","article-title":"Accurate Detection of \u03b1-Synuclein Seeds in Cerebrospinal Fluid from Isolated Rapid Eye Movement Sleep Behavior Disorder and Patients with Parkinson\u2019s Disease in the DeNovo Parkinson (DeNoPa) Cohort","volume":"38","author":"Weber","year":"2023","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1186\/s40478-021-01276-6","article-title":"Association between CSF alpha-synuclein seeding activity and genetic status in Parkinson\u2019s disease and dementia with Lewy bodies","volume":"9","author":"Brockmann","year":"2021","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1002\/acn3.772","article-title":"\u03b1-synuclein RT-QuIC in cerebrospinal fluid of LRRK2-linked Parkinson\u2019s disease","volume":"6","author":"Garrido","year":"2019","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/ana.25447","article-title":"\u03b1-Synuclein real-time quaking-induced conversion in the cerebrospinal fluid of uncertain cases of parkinsonism","volume":"85","author":"Green","year":"2019","journal-title":"Ann. Neurol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1093\/brain\/awab431","article-title":"Diagnostic value of cerebrospinal fluid alpha-synuclein seed quantification in synucleinopathies","volume":"145","author":"Poggiolini","year":"2022","journal-title":"Brain J. Neurol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s00401-020-02160-8","article-title":"Ultrasensitive RT-QuIC assay with high sensitivity and specificity for Lewy body-associated synucleinopathies","volume":"140","author":"Rossi","year":"2020","journal-title":"Acta Neuropathol."},{"key":"ref_31","first-page":"4125","article-title":"Real-time quaking-induced conversion assay is accurate for Lewy body diseases: A meta-analysis","volume":"43","author":"Wang","year":"2022","journal-title":"Neurol. Sci. Off. J. Ital. Neurol. Soc. Ital. Soc. Clin. Neurophysiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s40478-022-01388-7","article-title":"Performance of \u03b1Synuclein RT-QuIC in relation to neuropathological staging of Lewy body disease","volume":"10","author":"Hall","year":"2022","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_33","first-page":"374","article-title":"A rapid \u03b1-synuclein seed assay of Parkinson\u2019s disease CSF panel shows high diagnostic accuracy","volume":"8","author":"Ma","year":"2020","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.parkreldis.2022.05.006","article-title":"Combined CSF \u03b1-SYN RT-QuIC, CSF NFL and midbrain-pons planimetry in degenerative parkinsonisms: From bedside to bench, and back again","volume":"99","author":"Compta","year":"2022","journal-title":"Park. Relat. Disord."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13024-021-00491-y","article-title":"Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: The first assessment of assay reproducibility between two specialized laboratories","volume":"16","author":"Bargar","year":"2021","journal-title":"Mol. Neurodegener."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1002\/acn3.50897","article-title":"\u03b1-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies","volume":"6","author":"Bongianni","year":"2019","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1002\/mdc3.13710","article-title":"\u03b1-Synuclein Seed Amplification Assays in the Diagnosis of Synucleinopathies Using Cerebrospinal Fluid-A Systematic Review and Meta-Analysis","volume":"10","author":"Grossauer","year":"2023","journal-title":"Mov. Disord. Clin. Pract."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S1474-4422(20)30449-X","article-title":"Detection of \u03b1-synuclein in CSF by RT-QuIC in patients with isolated rapid-eye-movement sleep behaviour disorder: A longitudinal observational study","volume":"20","author":"Iranzo","year":"2021","journal-title":"Lancet Neurol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s40035-019-0164-x","article-title":"Efficient RT-QuIC seeding activity for \u03b1-synuclein in olfactory mucosa samples of patients with Parkinson\u2019s disease and multiple system atrophy","volume":"8","author":"Elia","year":"2019","journal-title":"Transl. Neurodegener."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1093\/brain\/awab005","article-title":"Alpha-synuclein seeds in olfactory mucosa of patients with isolated REM sleep behaviour disorder","volume":"144","author":"Stefani","year":"2021","journal-title":"Brain J. Neurol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"fcab045","DOI":"10.1093\/braincomms\/fcab045","article-title":"Alpha-synuclein seeds in olfactory mucosa and cerebrospinal fluid of patients with dementia with Lewy bodies","volume":"3","author":"Perra","year":"2021","journal-title":"Brain Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1002\/mds.28242","article-title":"Blinded RT-QuIC Analysis of \u03b1-Synuclein Biomarker in Skin Tissue From Parkinson\u2019s Disease Patients","volume":"35","author":"Manne","year":"2020","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_43","first-page":"1","article-title":"Skin \u03b1-Synuclein Aggregation Seeding Activity as a Novel Biomarker for Parkinson Disease","volume":"78","author":"Wang","year":"2020","journal-title":"JAMA Neurol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e2513","DOI":"10.1212\/WNL.0000000000011935","article-title":"In Vivo Diagnosis of Synucleinopathies: A Comparative Study of Skin Biopsy and RT-QuIC","volume":"96","author":"Donadio","year":"2021","journal-title":"Neurology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1002\/mds.28651","article-title":"RT-QuIC Detection of Pathological \u03b1-Synuclein in Skin Punches of Patients with Lewy Body Disease","volume":"36","author":"Mammana","year":"2021","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/s41531-021-00242-2","article-title":"Diagnostic value of skin RT-QuIC in Parkinson\u2019s disease: A two-laboratory study","volume":"7","author":"Kuzkina","year":"2021","journal-title":"NPJ Park. Dis"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1002\/mds.29340","article-title":"Dermal Real-Time Quaking-Induced Conversion Is a Sensitive Marker to Confirm Isolated Rapid Eye Movement Sleep Behavior Disorder as an Early \u03b1-Synucleinopathy","volume":"38","author":"Kuzkina","year":"2023","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.nbd.2019.05.002","article-title":"Detection of alpha-synuclein aggregates in gastrointestinal biopsies by protein misfolding cyclic amplification","volume":"129","author":"Fenyi","year":"2019","journal-title":"Neurobiol. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1002\/mds.28976","article-title":"Diagnostic Value of Salivary Real-Time Quaking-Induced Conversion in Parkinson\u2019s Disease and Multiple System Atrophy","volume":"37","author":"Luan","year":"2022","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1038\/s41591-023-02358-9","article-title":"Propagative \u03b1-synuclein seeds as serum biomarkers for synucleinopathies","volume":"29","author":"Okuzumi","year":"2023","journal-title":"Nat. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3949","DOI":"10.1111\/ene.16041","article-title":"Comparison of biospecimens for \u03b1-synuclein seed amplification assays in Parkinson\u2019s disease: A systematic review and network meta-analysis","volume":"30","author":"Zheng","year":"2023","journal-title":"Eur. J. Neurol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/mds.27907","article-title":"\u03b1-Synuclein real-time quaking-induced conversion in the submandibular glands of Parkinson\u2019s disease patients","volume":"35","author":"Manne","year":"2020","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s40478-021-01175-w","article-title":"Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson\u2019s disease and dementia with Lewy bodies","volume":"9","author":"Bargar","year":"2021","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/mds.29284","article-title":"Brain and Cerebrospinal Fluid \u03b1-Synuclein Real-Time Quaking-Induced Conversion Identifies Lewy Body Pathology in LRRK2-PD","volume":"38","author":"Garrido","year":"2023","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Hu, M.T. (2020). REM sleep behavior disorder (RBD). Neurobiol. Dis., 143.","DOI":"10.1016\/j.nbd.2020.104996"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1002\/mds.29766","article-title":"Detecting Misfolded \u03b1-Synuclein in Blood Years before the Diagnosis of Parkinson\u2019s Disease","volume":"39","author":"Kluge","year":"2024","journal-title":"Mov. Disord. Off. J. Mov. Disord. Soc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40035-022-00283-4","article-title":"Alpha-synuclein seeding shows a wide heterogeneity in multiple system atrophy","volume":"11","author":"Visanji","year":"2022","journal-title":"Transl. Neurodegener."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1515\/cclm-2023-1472","article-title":"Improving protocols for \u03b1-synuclein seed amplification assays: Analysis of preanalytical and analytical variables and identification of candidate parameters for seed quantification","volume":"62","author":"Mammana","year":"2024","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13024-023-00613-8","article-title":"Cerebrospinal fluid lipoproteins inhibit \u03b1-synuclein aggregation by interacting with oligomeric species in seed amplification assays","volume":"18","author":"Bellomo","year":"2023","journal-title":"Mol. Neurodegener."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e27949","DOI":"10.1016\/j.heliyon.2024.e27949","article-title":"Probing protein aggregation through spectroscopic insights and multimodal approaches: A comprehensive review for counteracting neurodegenerative disorders","volume":"10","author":"Bashir","year":"2024","journal-title":"Heliyon"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41531-023-00627-5","article-title":"CSF \u03b1-synuclein seed amplification kinetic profiles are associated with cognitive decline in Parkinson\u2019s disease","volume":"10","author":"Brockmann","year":"2024","journal-title":"NPJ Park. Dis"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1186\/s40478-021-01282-8","article-title":"High diagnostic performance of independent alpha-synuclein seed amplification assays for detection of early Parkinson\u2019s disease","volume":"9","author":"Russo","year":"2021","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Srivastava, A., Wang, Q., Orr\u00f9, C.D., Fernandez, M., Compta, Y., Ghetti, B., Zanusso, G., Zou, W.Q., Caughey, B., and Beauchemin, C.A.A. (2024). Enhanced quantitation of pathological \u03b1-synuclein in patient biospecimens by RT-QuIC seed amplification assays. 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