{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:51:59Z","timestamp":1789750319407,"version":"4.0.1"},"reference-count":49,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Sao Paulo Research Foundation, Brazil","award":["2020\/03.135-8"],"award-info":[{"award-number":["2020\/03.135-8"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["2022\/10.773-6"],"award-info":[{"award-number":["2022\/10.773-6"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["2021\/03.794-4"],"award-info":[{"award-number":["2021\/03.794-4"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["2021\/03792-1"],"award-info":[{"award-number":["2021\/03792-1"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["2023\/06.195-0"],"award-info":[{"award-number":["2023\/06.195-0"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["2021\/12.010-7"],"award-info":[{"award-number":["2021\/12.010-7"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["2021\/08.380-3"],"award-info":[{"award-number":["2021\/08.380-3"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["305091\/2021"],"award-info":[{"award-number":["305091\/2021"]}]},{"name":"The Sao Paulo Research Foundation, Brazil","award":["406442\/2022-3"],"award-info":[{"award-number":["406442\/2022-3"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2020\/03.135-8"],"award-info":[{"award-number":["2020\/03.135-8"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2022\/10.773-6"],"award-info":[{"award-number":["2022\/10.773-6"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2021\/03.794-4"],"award-info":[{"award-number":["2021\/03.794-4"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2021\/03792-1"],"award-info":[{"award-number":["2021\/03792-1"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2023\/06.195-0"],"award-info":[{"award-number":["2023\/06.195-0"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2021\/12.010-7"],"award-info":[{"award-number":["2021\/12.010-7"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["2021\/08.380-3"],"award-info":[{"award-number":["2021\/08.380-3"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["305091\/2021"],"award-info":[{"award-number":["305091\/2021"]}]},{"name":"The National Council for Scientific and Technological Development, Brazil","award":["406442\/2022-3"],"award-info":[{"award-number":["406442\/2022-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Preeclampsia (PE) is a hypertensive disorder of pregnancy and is associated with increases in soluble fms-like tyrosine kinase-1 (sFlt-1) and reductions in nitric oxide (NO) levels. Placental ischemia and hypoxia are hypothesized as initial pathophysiological events of PE. Nitrite (NO metabolite) may be recycled back to NO in ischemic and hypoxic tissues. Therefore, this study examined the sodium nitrite effects in an experimental model of PE. Pregnant rats received saline (Preg group) or sodium nitrite (Preg + Na-Nitrite group). Pregnant rats submitted to the placental ischemia received saline (RUPP group) or sodium nitrite (RUPP + Na-Nitrite group). Blood pressure, placental and fetal weights, and the number of pups were recorded. Plasma levels of NO metabolites and sFlt-1 were also determined. Vascular and endothelial functions were also measured. Blood pressure, placental and fetal weights, the number of pups, NO metabolites, sFlt-1 levels, vascular contraction, and endothelium-dependent vasodilation in the RUPP + Na-Nitrite rats were brought to levels comparable to those in Preg rats. In conclusion, sodium nitrite may counteract the reductions in NO and increases in sFlt-1 levels induced by the placental ischemia model of PE, thus suggesting that increased blood pressure and vascular and endothelial dysfunctions may be attenuated by sodium nitrite-derived NO.<\/jats:p>","DOI":"10.3390\/ijms252011051","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T08:09:08Z","timestamp":1728979748000},"page":"11051","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impaired Endothelium-Dependent Vasodilation and Increased Levels of Soluble Fms-like Tyrosine Kinase-1 Induced by Reduced Uterine Perfusion Pressure in Pregnant Rats: Evidence of Protective Effects with Sodium Nitrite Treatment in Preeclampsia"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9426-0019","authenticated-orcid":false,"given":"Maria Luiza Santos","family":"Da Silva","sequence":"first","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1skia Estela Biasotti","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9470-2008","authenticated-orcid":false,"given":"Laisla Zanetoni","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2610-7036","authenticated-orcid":false,"given":"Serginara David","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3165-1490","authenticated-orcid":false,"given":"Cristal de Jesus","family":"Toghi","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0348-6144","authenticated-orcid":false,"given":"Carlos Alan","family":"Dias-Junior","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijgo.12802","article-title":"The International Federation of Gynecology and Obstetrics (FIGO) initiative on pre-eclampsia: A pragmatic guide for first-trimester screening and prevention","volume":"145","author":"Poon","year":"2019","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"ref_2","first-page":"1","article-title":"Pre-eclampsia","volume":"9","author":"Dimitriadis","year":"2023","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e1552","DOI":"10.1542\/peds.2011-3093","article-title":"Cardiovascular Risk Factors in Children and Young Adults Born to Preeclamptic Pregnancies: A Systematic Review","volume":"129","author":"Davis","year":"2012","journal-title":"Pediatrics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1007\/s43032-020-00212-8","article-title":"Regulation of Uterine Spiral Artery Remodeling: A Review","volume":"27","author":"Albrecht","year":"2020","journal-title":"Reprod. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1161\/CIRCRESAHA.118.313276","article-title":"Preeclampsia: Pathophysiology, challenges, and perspectives","volume":"124","author":"Rana","year":"2019","journal-title":"Circ. Res."},{"key":"ref_6","first-page":"e31143","article-title":"Preeclampsia Incidence and Its Maternal and Neonatal Outcomes with Associated Risk Factors","volume":"14","author":"Khan","year":"2022","journal-title":"Cureus"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1055\/s-0037-1604471","article-title":"Preeclampsia","volume":"39","author":"Ramos","year":"2017","journal-title":"Rev. Bras. Ginecol. Obs."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1161\/HYPERTENSIONAHA.121.17567","article-title":"Circulating Maternal sFLT1 (Soluble fms-Like Tyrosine Kinase-1) Is Sufficient to Impair Spiral Arterial Remodeling in a Preeclampsia Mouse Model","volume":"78","author":"Vogtmann","year":"2021","journal-title":"Hypertension"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/j.1538-7836.2008.03259.x","article-title":"Vascular biology of preeclampsia","volume":"7","author":"Myatt","year":"2009","journal-title":"J. Thromb. Haemost."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1172\/JCI17189","article-title":"Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia","volume":"111","author":"Maynard","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/14767050500202493","article-title":"Evidence supporting that the excess of the sVEGFR-1 concentration in maternal plasma in preeclampsia has a uterine origin","volume":"18","author":"Bujold","year":"2005","journal-title":"J. Matern. Neonatal Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1093\/molehr\/4.4.377","article-title":"Evidence for the existence of a novel pregnancy-associated soluble variant of the vascular endothelial growth factor receptor, Flt-1","volume":"4","author":"Banks","year":"1998","journal-title":"Mol. Hum. Reprod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1095\/biolreprod59.6.1540","article-title":"A Vascular Endothelial Growth Factor Antagonist Is Produced by the Human Placenta and Released into the Maternal Circulation1","volume":"59","author":"Clark","year":"1998","journal-title":"Biol. Reprod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.preghy.2019.09.022","article-title":"NAMPT levels are inversely related to nitric oxide formation and positively related to soluble fms-like tyrosine kinase-1 levels in preeclampsia","volume":"18","author":"Pereira","year":"2019","journal-title":"Pregnancy Hypertens."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1161\/HYPERTENSIONAHA.108.115006","article-title":"Nitric Oxide Formation Is Inversely Related to Serum Levels of Antiangiogenic Factors Soluble Fms-Like Tyrosine Kinase-1 and Soluble Endogline in Preeclampsia","volume":"52","author":"Sandrim","year":"2008","journal-title":"Hypertension"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.niox.2016.05.004","article-title":"Sodium nitrite attenuates hypertension-in-pregnancy and blunts increases in soluble fms-like tyrosine kinase-1 and in vascular endothelial growth factor","volume":"57","author":"Nascimento","year":"2016","journal-title":"Nitric Oxide"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.niox.2018.08.009","article-title":"Nitrite mediated vasorelaxation in human chorionic plate vessels is enhanced by hypoxia and dependent on the NO-sGC-cGMP pathway","volume":"80","author":"Tropea","year":"2018","journal-title":"Nitric Oxide"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.niox.2019.11.006","article-title":"Nitric oxide signaling in pregnancy and preeclampsia","volume":"95","author":"Sutton","year":"2019","journal-title":"Nitric Oxide"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/bcp.12301","article-title":"The nitric oxide pathway and possible therapeutic options in pre-eclampsia","volume":"78","author":"Johal","year":"2013","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Barbosa, P.O., Tanus-Santos, J.E., Cavalli, R.d.C., Bengtsson, T., Montenegro, M.F., and Sandrim, V.C. (2024). The Nitrate-Nitrite-Nitric Oxide Pathway: Potential Role in Mitigating Oxidative Stress in Hypertensive Disorders of Pregnancy. Nutrients, 16.","DOI":"10.20944\/preprints202404.0781.v1"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1161\/01.HYP.37.4.1191","article-title":"Reduced Uterine Perfusion Pressure During Pregnancy in the Rat is Associated with Increases in Arterial Pressure and Changes in Renal Nitric Oxide","volume":"37","author":"Alexander","year":"2001","journal-title":"Hypertension"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"H89","DOI":"10.1152\/ajpheart.00056.2024","article-title":"Reduced uterine perfusion pressure as a model for preeclampsia and fetal growth restriction in murine: A systematic review and meta-analysis","volume":"327","author":"Taal","year":"2024","journal-title":"Am. J. Physiol. Circ. Physiol."},{"key":"ref_23","first-page":"124","article-title":"Circulating Angiogenic Factor Levels in Hypertensive Disorders of Pregnancy","volume":"1","author":"Thadhani","year":"2022","journal-title":"NEJM \u00c9vid."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1161\/HYPERTENSIONAHA.122.20739","article-title":"Specific HIF-2\u03b1 (Hypoxia-Inducible Factor-2) Inhibitor PT2385 Mitigates Placental Dysfunction In Vitro and in a Rat Model of Preeclampsia (RUPP)","volume":"80","author":"Colson","year":"2023","journal-title":"Hypertension"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1095\/biolreprod.104.036715","article-title":"Reduced Uteroplacental Perfusion Alters Uterine Arcuate Artery Function in the Pregnant Sprague-Dawley Rat1","volume":"72","author":"Anderson","year":"2005","journal-title":"Biol. Reprod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/S0008-6363(00)00087-0","article-title":"Vascular function in preeclampsia","volume":"47","author":"VanWijk","year":"2000","journal-title":"Cardiovasc. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1161\/01.HYP.35.1.367","article-title":"Decreased Endothelium-Dependent Vascular Relaxation During Reduction of Uterine Perfusion Pressure in Pregnant Rat","volume":"35","author":"Crews","year":"2000","journal-title":"Hypertension"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/micc.12079","article-title":"Vascular Dysfunction in Preeclampsia","volume":"21","author":"Brennan","year":"2014","journal-title":"Microcirculation"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.preghy.2011.11.005","article-title":"Increased myogenic reactivity of uterine arteries from pregnant rats with reduced uterine perfusion pressure","volume":"2","author":"Reho","year":"2011","journal-title":"Pregnancy Hypertens."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"H661","DOI":"10.1152\/ajpheart.00202.2020","article-title":"Vascular mechanisms and molecular targets in hypertensive pregnancy and preeclampsia","volume":"319","author":"Qu","year":"2020","journal-title":"Am. J. Physiol. Circ. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Opichka, M.A., Rappelt, M.W., Gutterman, D.D., Grobe, J.L., and McIntosh, J.J. (2021). Vascular Dysfunction in Preeclampsia. Cells, 10.","DOI":"10.3390\/cells10113055"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1080\/14728222.2017.1310840","article-title":"The potential of stimulating nitric oxide formation in the treatment of hypertension","volume":"21","author":"Pinheiro","year":"2017","journal-title":"Expert Opin. Ther. Targets"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.redox.2012.11.005","article-title":"Nitrite: A physiological store of nitric oxide and modulator of mitochondrial function","volume":"1","author":"Shiva","year":"2013","journal-title":"Redox Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1016\/j.placenta.2011.08.008","article-title":"Nitric oxide generation affects pro- and anti-angiogenic growth factor expression in primary human trophoblast","volume":"32","author":"Groesch","year":"2011","journal-title":"Placenta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"H541","DOI":"10.1152\/ajpheart.01113.2007","article-title":"Pathophysiology of hypertension during preeclampsia: Linking placental ischemia with endothelial dysfunction","volume":"294","author":"Gilbert","year":"2008","journal-title":"Am. J. Physiol. Circ. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"46","DOI":"10.14390\/jsshp.HRP2017-014","article-title":"Hypoxia in the pathogenesis of preeclampsia","volume":"5","author":"Matsubara","year":"2017","journal-title":"Hypertens. Res. Pregnancy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1017\/S204017441900014X","article-title":"Preeclampsia link to gestational hypoxia","volume":"10","author":"Tong","year":"2019","journal-title":"J. Dev. Orig. Heal. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s11906-022-01225-1","article-title":"Hypoxia-Inducible Factor 1 and Preeclampsia: A New Perspective","volume":"24","author":"Albogami","year":"2022","journal-title":"Curr. Hypertens. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1210\/jc.2005-0078","article-title":"Molecular Evidence of Placental Hypoxia in Preeclampsia","volume":"90","author":"Soleymanlou","year":"2005","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0076-6879(07)35024-6","article-title":"Hypoxia-Inducible Factor-1\u03b1 Under the Control of Nitric Oxide","volume":"Volume 435","author":"Zhou","year":"2007","journal-title":"Methods in Enzymology"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sanchez-Aranguren, L.C., Prada, C.E., Ri\u00e3no-Medina, C.E., and Lopez, M. (2014). Endothelial dysfunction and preeclampsia: Role of oxidative stress. Front. Physiol., 5.","DOI":"10.3389\/fphys.2014.00372"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.cjca.2015.09.003","article-title":"Pregnancy and Thoracic Aortic Disease: Managing the Risks","volume":"32","author":"Wanga","year":"2015","journal-title":"Can. J. Cardiol."},{"key":"ref_43","first-page":"86","article-title":"Maternal mortality attributable to vascular dissection and rupture in the Netherlands: A nationwide confidential enquiry","volume":"119","author":"Schutte","year":"2011","journal-title":"BJOG: Int. J. Obstet. Gynaecol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"R505","DOI":"10.1152\/ajpregu.00137.2016","article-title":"Restoring placental growth factor-soluble fms-like tyrosine kinase-1 balance reverses vascular hyper-reactivity and hypertension in pregnancy","volume":"311","author":"Zhu","year":"2016","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.bcp.2017.09.005","article-title":"Angiogenic imbalance and diminished matrix metalloproteinase-2 and -9 underlie regional decreases in uteroplacental vascularization and feto-placental growth in hypertensive pregnancy","volume":"146","author":"Chen","year":"2017","journal-title":"Biochem. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1111\/jcmm.12106","article-title":"Antihypertensive effects of inducible nitric oxide synthase inhibition in experimental pre-eclampsia","volume":"17","author":"Amaral","year":"2013","journal-title":"J. Cell. Mol. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.redox.2015.06.009","article-title":"Consistent antioxidant and antihypertensive effects of oral sodium nitrite in DOCA-salt hypertension","volume":"5","author":"Amaral","year":"2015","journal-title":"Redox Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.niox.2019.01.015","article-title":"Mechanisms impairing blood pressure responses to nitrite and nitrate","volume":"85","author":"Pinheiro","year":"2019","journal-title":"Nitric Oxide"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.clinbiochem.2010.12.011","article-title":"A simple and inexpensive automated technique for measurement of serum nitrite\/nitrate","volume":"44","author":"Tatsch","year":"2010","journal-title":"Clin. Biochem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/20\/11051\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:13:28Z","timestamp":1760112808000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/20\/11051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,15]]},"references-count":49,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["ijms252011051"],"URL":"https:\/\/doi.org\/10.3390\/ijms252011051","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,15]]}}}