{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T21:26:25Z","timestamp":1774905985340,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32301644"],"award-info":[{"award-number":["32301644"]}]},{"name":"National Natural Science Foundation of China","award":["LQ20C150003"],"award-info":[{"award-number":["LQ20C150003"]}]},{"name":"National Natural Science Foundation of China","award":["22052307-Y"],"award-info":[{"award-number":["22052307-Y"]}]},{"name":"National Natural Science Foundation of China","award":["57"],"award-info":[{"award-number":["57"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["32301644"],"award-info":[{"award-number":["32301644"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LQ20C150003"],"award-info":[{"award-number":["LQ20C150003"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["22052307-Y"],"award-info":[{"award-number":["22052307-Y"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["57"],"award-info":[{"award-number":["57"]}]},{"name":"Fundamental Research Funds from Zhejiang Sci-Tech University","award":["32301644"],"award-info":[{"award-number":["32301644"]}]},{"name":"Fundamental Research Funds from Zhejiang Sci-Tech University","award":["LQ20C150003"],"award-info":[{"award-number":["LQ20C150003"]}]},{"name":"Fundamental Research Funds from Zhejiang Sci-Tech University","award":["22052307-Y"],"award-info":[{"award-number":["22052307-Y"]}]},{"name":"Fundamental Research Funds from Zhejiang Sci-Tech University","award":["57"],"award-info":[{"award-number":["57"]}]},{"name":"Undergraduate Research and Innovation Program of Zhejiang Sci-Tech University","award":["32301644"],"award-info":[{"award-number":["32301644"]}]},{"name":"Undergraduate Research and Innovation Program of Zhejiang Sci-Tech University","award":["LQ20C150003"],"award-info":[{"award-number":["LQ20C150003"]}]},{"name":"Undergraduate Research and Innovation Program of Zhejiang Sci-Tech University","award":["22052307-Y"],"award-info":[{"award-number":["22052307-Y"]}]},{"name":"Undergraduate Research and Innovation Program of Zhejiang Sci-Tech University","award":["57"],"award-info":[{"award-number":["57"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Gray mold caused by Botrytis elliptica is one of the most determinative factors of lily growth and has become a major threat to lily productivity. However, the nature of the lily B. elliptica interaction remains largely unknown. Here, comparative transcriptomic and metabolomic were used to investigate the defense responses of resistant (\u2018Sorbonne\u2019) and susceptible (\u2018Tresor\u2019) lily cultivars to B. elliptica infection at 24 hpi. In total, 1326 metabolites were identified in \u2018Sorbonne\u2019 and \u2018Tresor\u2019 after infection, including a large number of phenylpropanoids. Specifically, the accumulation of four phenylpropanes, including eriodictyol, hesperetin, ferulic acid, and sinapyl alcohol, was significantly upregulated in the B. elliptica-infected \u2018Sorbonne\u2019 compared with the infected \u2018Tresor\u2019, and these phenylpropanes could significantly inhibit B. elliptica growth. At the transcript level, higher expression levels of F3\u2032M, COMT, and CAD led to a higher content of resistance-related phenylpropanes (eriodictyol, ferulic acid, and sinapyl alcohol) in \u2018Sorbonne\u2019 following B. elliptica infection. It can be assumed that these phenylpropanes cause the resistance difference between \u2018Sorbonne\u2019 and \u2018Tresor\u2019, and could be the potential marker metabolites for gray mold resistance in the lily. Further transcriptional regulatory network analysis suggested that members of the AP2\/ERF, WRKY, Trihelix, and MADS-M-type families positively regulated the biosynthesis of resistance-related phenylpropanes. Additionally, the expression patterns of genes involved in phenylpropanoid biosynthesis were confirmed using qRT-PCR. Therefore, we speculate that the degree of gray mold resistance in the lily is closely related to the contents of phenylpropanes and the transcript levels of the genes in the phenylpropanoid biosynthesis pathway. Our results not only improve our understanding of the lily\u2019s resistance mechanisms against B. elliptica, but also facilitate the genetic improvement of lily cultivars with gray mold resistance.<\/jats:p>","DOI":"10.3390\/ijms252011068","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T08:09:08Z","timestamp":1728979748000},"page":"11068","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Critical Role of Phenylpropanoid Biosynthesis Pathway in Lily Resistance Against Gray Mold"],"prefix":"10.3390","volume":"25","author":[{"given":"Qi","family":"Cui","sequence":"first","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3763-8155","authenticated-orcid":false,"given":"Xinran","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Hu","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongfeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Abozeid","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9130-6272","authenticated-orcid":false,"given":"Ziming","family":"Ren","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1914-857X","authenticated-orcid":false,"given":"Danqing","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongze","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anhua","family":"Qin","sequence":"additional","affiliation":[{"name":"Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1094\/PHYTO-09-22-0357-SA","article-title":"Exploring the relationship between pattern-triggered immunity and quantitative resistance to Xanthomonas vasicola pv. vasculorum in maize","volume":"113","author":"Mullens","year":"2023","journal-title":"Phytopathology"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Doehlemann, G., \u00d6kmen, B., Zhu, W., and Sharon, A. 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