{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:34:31Z","timestamp":1792802071024,"version":"4.5.0"},"reference-count":43,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Science, and Technological Development of the Republic of Serbia","award":["451-03-65\/2024-03\/200113"],"award-info":[{"award-number":["451-03-65\/2024-03\/200113"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Lipopolysaccharide (LPS) is known to induce oxidative stress and inflammation, leading to significant damage in cardiac tissues. This study investigates the protective effects of melatonin (MLT) against LPS-induced oxidative damage, inflammation, and apoptosis in rat heart tissue. Rats were divided into four groups (n = 6 per group): control, melatonin-treated, LPS-treated, and LPS + melatonin-treated. Oxidative stress markers, including thiobarbituric acid-reactive substances (TBARSs) and advanced oxidation protein products (AOPPs), were measured. Additionally, inflammatory markers, such as interleukin-6 (IL-6) levels, inducible nitric oxide synthase (iNOS) and nitric oxide (NO) content, and apoptotic markers, caspase-3, caspase-9, and acidic DNase activity, were evaluated. LPS treatment significantly increased TBARS, AOPP, and IL-6 levels, as well as the activity of caspase-3, acidic DNase and iNOS and NO content compared to the control group. Co-treatment with melatonin significantly reduced the levels of TBARS and AOPP levels, and caspase-3 and acidic DNase activities nearly matched those of the control group, while caspse-9 was still slightly increased. Interestingly, IL-6, iNOS and NO levels were significantly decreased but did not fully match the values in the control group. Melatonin mitigates LPS-induced oxidative stress, inflammation, and apoptosis in rat heart tissue by affecting all studied parameters, demonstrating its potential as a therapeutic agent for conditions characterized by oxidative stress and inflammation. Further research is warranted to explore the clinical applications of melatonin in cardiovascular diseases.<\/jats:p>","DOI":"10.3390\/ijms252011088","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T09:14:48Z","timestamp":1729242888000},"page":"11088","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Melatonin Mediates Cardiac Tissue Damage under Septic Conditions Induced by Lipopolysaccharide"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8898-948X","authenticated-orcid":false,"given":"Milan","family":"Lazarevi\u0107","sequence":"first","affiliation":[{"name":"Department of Immunology, Medical Faculty of Ni\u0161, University of Nis, 18000 Ni\u0161, Serbia"},{"name":"Clinic for Cardiovascular and Transplant Surgery, University Clinical Centre of Nis, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milo\u0161","family":"Kosti\u0107","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical Faculty of Ni\u0161, University of Nis, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1074-8592","authenticated-orcid":false,"given":"Tanja","family":"D\u017eopali\u0107","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical Faculty of Ni\u0161, University of Nis, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danka","family":"Sokolovi\u0107","sequence":"additional","affiliation":[{"name":"Blood Transfusion Institute Ni\u0161, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zorica","family":"Lazarevi\u0107","sequence":"additional","affiliation":[{"name":"Clinic for Cardiovascular and Transplant Surgery, University Clinical Centre of Nis, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Milovanovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Unisversity of Pri\u0161tina, 38220 Kosovska Mitrovica, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanja","family":"Ni\u010dkovi\u0107","sequence":"additional","affiliation":[{"name":"Clinic of Gastroenterohepatology, University Clinical Centre of Ni\u0161, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6586-6428","authenticated-orcid":false,"given":"Du\u0161an","family":"Sokolovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry, Faculty of Medicine, University of Ni\u0161, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Koc, F., Tekeli, M.Y., Kanbur, M., Karayigit, M.\u00d6., and Liman, B.C. 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