{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:43:53Z","timestamp":1790891033208,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T00:00:00Z","timestamp":1729209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-65\/2024-03\/200111"],"award-info":[{"award-number":["451-03-65\/2024-03\/200111"]}]},{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-65\/2024-03\/200124"],"award-info":[{"award-number":["451-03-65\/2024-03\/200124"]}]},{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["7743504"],"award-info":[{"award-number":["7743504"]}]},{"name":"Science Fund of the Republic of Serbia, The Program Ideas","award":["451-03-65\/2024-03\/200111"],"award-info":[{"award-number":["451-03-65\/2024-03\/200111"]}]},{"name":"Science Fund of the Republic of Serbia, The Program Ideas","award":["451-03-65\/2024-03\/200124"],"award-info":[{"award-number":["451-03-65\/2024-03\/200124"]}]},{"name":"Science Fund of the Republic of Serbia, The Program Ideas","award":["7743504"],"award-info":[{"award-number":["7743504"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Phytochemical composition and antimicrobial, antibiofilm, and antioxidant effects of a newly described Himalayan lichen Placidium deosaiense Usman and Khalid growing in Pakistan were investigated. HPLC\u2013DAD methods were used for identification of secondary metabolites in acetone and methanol extracts. The total phenolics content was measured using a spectrophotometric method. The study investigated the antioxidant (DPPH-scavenging activity assay and reducing-power assay), antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)), and antibiofilm (inhibition of biofilm formation and reduction in mature biofilm) activities of extracts of the lichen P. deosaiense and isolated parietin. The chemical constituents olivetol, olivetolic acid, haematommic acid, fallacinol, and parietin were identified as major compounds in the tested extracts of the lichen. Parietin was isolated from the acetone extract on a separation column. The methanol extract had higher values of TPC (21.67 mg GAE\/g) than the acetone extract. Isolated parietin showed the best antioxidant activity measures, according to the DPPH-scavenging activity assay (IC50 = 51.616 \u03bcg\/mL) and reducing-power assay. Although the extracts showed the best antibacterial activity (especially against Proteus mirabilis ATCC 12453), parietin demonstrated superior antibiofilm activity (especially against Staphylococcus aureus ATCC 25923). This is the first report on the phytochemical composition of the lichen Placidium deosaiense and the first description of the chemical composition of some of the 45 species of the genus Placidium. This research will pave the way for further exploration of new activities of this lichen and its metabolites, which are important for medicine and pharmacy.<\/jats:p>","DOI":"10.3390\/ijms252011203","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T05:03:49Z","timestamp":1729227829000},"page":"11203","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Phytochemical Analysis and Antioxidant, Antimicrobial, and Antibiofilm Effects of a New Himalayan Lichen Placidium deosaiense Usman and Khalid Growing in Pakistan"],"prefix":"10.3390","volume":"25","author":[{"given":"Anja","family":"Manojlovi\u0107","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5635-8031","authenticated-orcid":false,"given":"Abdul Nasir","family":"Khalid","sequence":"additional","affiliation":[{"name":"Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore 54590, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3490-058X","authenticated-orcid":false,"given":"Muhammad","family":"Usman","sequence":"additional","affiliation":[{"name":"Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Lahore 54590, Pakistan"},{"name":"School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olgica","family":"Stefanovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevena","family":"\u0110uki\u0107","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9170-1790","authenticated-orcid":false,"given":"Nedeljko","family":"Manojlovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7300-5385","authenticated-orcid":false,"given":"Jovica","family":"Tomovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia"},{"name":"Center for Harm Reduction of Biological and Chemical Hazards, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14707","DOI":"10.3390\/ijms131114707","article-title":"Biological Activities of Toninia candida and Usnea barbata Together with Their Norstictic Acid and Usnic Acid Constituents","volume":"13","year":"2012","journal-title":"Int. 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