{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:31:28Z","timestamp":1760146288002,"version":"build-2065373602"},"reference-count":69,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T00:00:00Z","timestamp":1729900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-14-00194"],"award-info":[{"award-number":["19-14-00194"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Many phytopathogens\u2019 gene products that contribute to plant\u2013pathogen interactions remain unexplored. In one of the most harmful phytopathogenic bacterium Pectobacterium atrosepticum (Pba), phosphonate-related genes have been previously shown to be among the most upregulated following host plant colonization. However, phosphonates, compounds characterized by a carbon\u2013phosphorus bond in their composition, have not been described in Pectobacterium species and other phytopathogenic bacteria, with the exception of Pseudomonas syringae and Pantoea ananatis. Our study aimed to determine whether Pba synthesizes extracellular phosphonates and, if so, to analyze their physiological functions. We demonstrated that Pba produces two types of extracellular phosphonates: 2-diethoxyphosphorylethanamine and phenylphosphonic acid. Notably, such structures have not been previously described among natural phosphonates. The production of Pba phosphonates was shown to be positively regulated by quorum sensing and in the presence of pectic compounds. Pba phosphonates were found to have a positive effect on Pba stress resistance and a negative effect on Pba virulence. The discovered Pba phosphonates are discussed as metabolites that enable Pba to control its \u201charmful properties\u201d, thereby maintaining its ecological niche (the host plant) in a relatively functional state for an extended period.<\/jats:p>","DOI":"10.3390\/ijms252111516","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T04:15:02Z","timestamp":1730088902000},"page":"11516","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phosphonates of Pectobacterium atrosepticum: Discovery and Role in Plant\u2013Pathogen Interactions"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5527-4696","authenticated-orcid":false,"given":"Olga","family":"Parfirova","sequence":"first","affiliation":[{"name":"Kazan Institute of Biochemistry and Biophysics, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420111 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0301-8807","authenticated-orcid":false,"given":"Polina","family":"Mikshina","sequence":"additional","affiliation":[{"name":"Kazan Institute of Biochemistry and Biophysics, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420111 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1618-5479","authenticated-orcid":false,"given":"Olga","family":"Petrova","sequence":"additional","affiliation":[{"name":"Kazan Institute of Biochemistry and Biophysics, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420111 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1470-8136","authenticated-orcid":false,"given":"Andrey","family":"Smolobochkin","sequence":"additional","affiliation":[{"name":"Arbuzov Institute of Organic and Physical Chemistry, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420088 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Pashagin","sequence":"additional","affiliation":[{"name":"Kazan Institute of Biochemistry and Biophysics, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420111 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Burilov","sequence":"additional","affiliation":[{"name":"Arbuzov Institute of Organic and Physical Chemistry, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420088 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Gorshkov","sequence":"additional","affiliation":[{"name":"Kazan Institute of Biochemistry and Biophysics, Federal Research Center \u201cKazan Scientific Center of the Russian Academy of Sciences\u201d, 420111 Kazan, Russia"},{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/j.1364-3703.2012.00804.x","article-title":"Top 10 plant pathogenic bacteria in molecular plant pathology","volume":"13","author":"Mansfield","year":"2012","journal-title":"Mol. 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