{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:19:47Z","timestamp":1788610787259,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2024,10,27]],"date-time":"2024-10-27T00:00:00Z","timestamp":1729987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["23CDA1053102"],"award-info":[{"award-number":["23CDA1053102"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["AA028435"],"award-info":[{"award-number":["AA028435"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["AA024337"],"award-info":[{"award-number":["AA024337"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute on Alcohol Abuse and Alcoholism","award":["23CDA1053102"],"award-info":[{"award-number":["23CDA1053102"]}]},{"name":"National Institute on Alcohol Abuse and Alcoholism","award":["AA028435"],"award-info":[{"award-number":["AA028435"]}]},{"name":"National Institute on Alcohol Abuse and Alcoholism","award":["AA024337"],"award-info":[{"award-number":["AA024337"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Prenatal ethanol exposure can cause a broad range of abnormalities in newborns known as Fetal Alcohol Spectrum Disorder (FASD). Despite significant progress in understanding the disease mechanisms of FASD, there remains a strong global need for effective therapies. To evaluate the therapeutic potential of sulforaphane (SFN), an active compound extracted from cruciferous vegetables, in preventing FASD, ethanol-exposed zebrafish embryos were pretreated, co-treated, or post-treated with various concentrations of SFN. The FASD-like morphological features, survival rate, hatching rate, and vascular development were then assessed in the zebrafish embryos. It was found that pretreatment with 2 \u03bcM SFN during 3\u201324 hpf had no noticeable protective effects against teratogenicity induced by subsequent 1.5% ethanol exposure during 24\u201348 hpf. In contrast, co-treatment with 2 \u03bcM SFN and 1.5% ethanol during 3\u201324 hpf significantly alleviated a range of ethanol-induced malformations, including reduced body length, small eyes, reduced brain size, small otic vesicle, small jaw, and pericardial edema. Post-treatment with 3 \u03bcM SFN for 4 days following 1.5% ethanol exposure during 3\u201324 hpf also significantly reduced the characteristic features of FASD, decreasing the mortality rate and restoring body length, eye size, brain size, and otic vesicle circumference. Moreover, we found that ethanol, even at a low dose (0.5%), causes vascular development deficit in the zebrafish embryos, which were also largely rescued by SFN treatment. These data indicated that SFN has great potential to be used in the prevention and treatment of FASD.<\/jats:p>","DOI":"10.3390\/ijms252111529","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T04:15:02Z","timestamp":1730088902000},"page":"11529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sulforaphane Attenuates Ethanol-Induced Teratogenesis and Dysangiogenesis in Zebrafish Embryos"],"prefix":"10.3390","volume":"25","author":[{"given":"Zhijian","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha 410010, China"},{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kansas City, KS 66126, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Louisville Health Sciences Center, Louisville, KY 40292, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0120-9343","authenticated-orcid":false,"given":"Liang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kansas City, KS 66126, USA"},{"name":"Institute of Reproductive and Developmental Sciences, The University of Kansas Medical Center, Kansas City, KS 66126, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,27]]},"reference":[{"key":"ref_1","first-page":"279","article-title":"Fetal alcohol spectrum disorders","volume":"72","author":"Wattendorf","year":"2005","journal-title":"Am. 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