{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:34:31Z","timestamp":1787225671156,"version":"build-2736575974"},"reference-count":90,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2024,10,27]],"date-time":"2024-10-27T00:00:00Z","timestamp":1729987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Burroughs Wellcome Fund Next Gen Pregnancy Research Grant","award":["1022519"],"award-info":[{"award-number":["1022519"]}]},{"name":"Burroughs Wellcome Fund Next Gen Pregnancy Research Grant","award":["NIH\/NIGMS 2P20GM103620"],"award-info":[{"award-number":["NIH\/NIGMS 2P20GM103620"]}]},{"name":"Burroughs Wellcome Fund Next Gen Pregnancy Research Grant","award":["NIH\/NHLBI 1RO1HL160980"],"award-info":[{"award-number":["NIH\/NHLBI 1RO1HL160980"]}]},{"name":"Sanford Research and Center of Biomedical Research Excellence Award","award":["1022519"],"award-info":[{"award-number":["1022519"]}]},{"name":"Sanford Research and Center of Biomedical Research Excellence Award","award":["NIH\/NIGMS 2P20GM103620"],"award-info":[{"award-number":["NIH\/NIGMS 2P20GM103620"]}]},{"name":"Sanford Research and Center of Biomedical Research Excellence Award","award":["NIH\/NHLBI 1RO1HL160980"],"award-info":[{"award-number":["NIH\/NHLBI 1RO1HL160980"]}]},{"name":"University of South Dakota Sanford School of Medicine Academy of Medical Student Scholarship Committee","award":["1022519"],"award-info":[{"award-number":["1022519"]}]},{"name":"University of South Dakota Sanford School of Medicine Academy of Medical Student Scholarship Committee","award":["NIH\/NIGMS 2P20GM103620"],"award-info":[{"award-number":["NIH\/NIGMS 2P20GM103620"]}]},{"name":"University of South Dakota Sanford School of Medicine Academy of Medical Student Scholarship Committee","award":["NIH\/NHLBI 1RO1HL160980"],"award-info":[{"award-number":["NIH\/NHLBI 1RO1HL160980"]}]},{"name":"Sanford Research","award":["1022519"],"award-info":[{"award-number":["1022519"]}]},{"name":"Sanford Research","award":["NIH\/NIGMS 2P20GM103620"],"award-info":[{"award-number":["NIH\/NIGMS 2P20GM103620"]}]},{"name":"Sanford Research","award":["NIH\/NHLBI 1RO1HL160980"],"award-info":[{"award-number":["NIH\/NHLBI 1RO1HL160980"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the parent, gestational diabetes mellitus (GDM) causes both hyperglycemia and hyperlipidemia. Despite excess lipid availability, infants exposed to GDM are at risk for essential long-chain polyunsaturated fatty acid (LCPUFA) deficiency. Isotope studies have confirmed less LCPUFA transfer from the parent to the fetus, but how diabetic fuels impact placental fatty acid (FA) uptake and lipid droplet partitioning is not well-understood. We evaluated the effects of high glucose conditions, high lipid conditions, and their combination on trophoblast growth, viability, mitochondrial bioenergetics, BODIPY-labeled fatty acid (FA) uptake, and lipid droplet dynamics. The addition of four carbons or one double bond to FA acyl chains dramatically affected the uptake in both BeWo and primary isolated cytotrophoblasts (CTBs). The uptake was further impacted by media exposure. The combination-exposed trophoblasts had more mitochondrial protein (p = 0.01), but impaired maximal and spare respiratory capacities (p &lt; 0.001 and p &lt; 0.0001), as well as lower viability (p = 0.004), due to apoptosis. The combination-exposed trophoblasts had unimpaired uptake of BODIPY C12 but had significantly less whole-cell and lipid droplet uptake of BODIPY C16, with an altered lipid droplet count, area, and subcellular localization, whereas these differences were not seen with individual high glucose or lipid exposure. These findings bring us closer to understanding how GDM perturbs active FA transport to increase the risk of adverse outcomes from placental and neonatal lipid accumulation alongside LCPUFA deficiency.<\/jats:p>","DOI":"10.3390\/ijms252111534","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T04:15:02Z","timestamp":1730088902000},"page":"11534","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Gestational Diabetes-like Fuels Impair Mitochondrial Function and Long-Chain Fatty Acid Uptake in Human Trophoblasts"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8765-5103","authenticated-orcid":false,"given":"Kyle M.","family":"Siemers","sequence":"first","affiliation":[{"name":"Sanford School of Medicine, University of South Dakota, 414 E. Clark Street, Vermillion, SD 57069, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6820-0605","authenticated-orcid":false,"given":"Lisa A.","family":"Joss-Moore","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Utah, 295 Chipeta Way, 2N131, Salt Lake City, UT 84108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5966-669X","authenticated-orcid":false,"given":"Michelle L.","family":"Baack","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Neonatology, Sanford School of Medicine, University of South Dakota, 1400 W. 22nd St., Sioux Falls, SD 57105, USA"},{"name":"Environmental Influences on Health and Disease Group, Sanford Research, 2301 E. 60th St., Sioux Falls, SD 57104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Plows, J.F., Stanley, J.L., Baker, P.N., Reynolds, C.M., and Vickers, M.H. 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