{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:33:39Z","timestamp":1760146419788,"version":"build-2065373602"},"reference-count":66,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biological Breeding-Major Projects","award":["2023ZD04062"],"award-info":[{"award-number":["2023ZD04062"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A transcription-aided selection (TAS) strategy is proposed in this paper, which utilizes the positive regulatory roles of genes involved in the plant immunity pathways to screen crops with high disease resistance. Increased evidence has demonstrated that upon pathogen attack, the expression of diverse genes involved in salicylic acid (SA)-mediated SAR are differentially expressed and transcriptionally regulated. The paper discusses the molecular mechanisms of the SA signaling pathway, which plays a central role in plant immunity, and identifies differentially expressed genes (DEGs) that could be targeted for transcriptional detection. We have conducted a series of experiments to test the TAS strategy and found that the level of GmSAGT1 expression is highly correlated with soybean downy mildew (SDM) resistance with a correlation coefficient R2 = 0.7981. Using RT-PCR, we screened 2501 soybean germplasms and selected 26 collections with higher levels of both GmSAGT1 and GmPR1 (Pathogenesis-related proteins1) gene expression. Twenty-three out of the twenty-six lines were inoculated with Peronospora manshurica (Pm) in a greenhouse. Eight showed HR (highly resistant), four were R (resistant), five were MR (moderately resistant), three were S (susceptible), and three were HS (highly susceptible). The correlation coefficient R2 between the TAS result and Pm inoculation results was 0.7035, indicating a satisfactory consistency. The authors anticipate that TAS provides an effective strategy for screening crops with broad-spectrum and long-lasting resistance.<\/jats:p>","DOI":"10.3390\/ijms252211879","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T06:30:48Z","timestamp":1730788248000},"page":"11879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcription-Aided Selection (TAS) for Crop Disease Resistance: Strategy and Evidence"],"prefix":"10.3390","volume":"25","author":[{"given":"Jiu","family":"Huang","sequence":"first","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxun","family":"Qi","sequence":"additional","affiliation":[{"name":"Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Li","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Yu","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erte","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/s41586-021-03316-6","article-title":"Pattern-recognition receptors are required for NLR-mediated plant immunity","volume":"592","author":"Yuan","year":"2021","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/BF00032062","article-title":"The integrated concept of disease resistance: A new view including horizontal and vertical resistance in plants","volume":"26","author":"Parlevliet","year":"1977","journal-title":"Euphytica"},{"key":"ref_4","unstructured":"Vanderplank, J.E. (1968). Disease Resistance in Plants, Academic Press."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/s00425-024-04511-1","article-title":"Advancements in genetically modified insect pest-resistant crops in India","volume":"260","author":"Rakesh","year":"2024","journal-title":"Planta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s00425-020-03372-8","article-title":"Genetically modified crops: Current status and future prospects","volume":"251","author":"Kumar","year":"2020","journal-title":"Planta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1104\/pp.104.4.1109","article-title":"Systemic acquired resistance","volume":"104","author":"Ryals","year":"1994","journal-title":"Plant Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.2307\/3870231","article-title":"Systemic acquired resistance","volume":"8","author":"Ryals","year":"1996","journal-title":"Plant Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1146\/annurev.phyto.42.040803.140421","article-title":"Systemic acquired resistance","volume":"42","author":"Durrant","year":"2004","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"179","DOI":"10.4161\/psb.1.4.3221","article-title":"Systemic acquired resistance","volume":"1","author":"Conrath","year":"2006","journal-title":"Plant Signal. Behav."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1111\/j.1744-7909.2011.01043.x","article-title":"Salicylic acid and its function in plant immunity","volume":"53","author":"An","year":"2011","journal-title":"J. Integr. Plant Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1146\/annurev-arplant-042811-105606","article-title":"Systemic acquired resistance: Turning local infection into global defense","volume":"64","author":"Fu","year":"2013","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/0042-6822(79)90019-9","article-title":"Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco","volume":"99","author":"White","year":"1979","journal-title":"Virology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1093\/plcell\/koad329","article-title":"Salicylic acid in plant immunity and beyond","volume":"36","author":"Spoel","year":"2024","journal-title":"Plant Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3321","DOI":"10.1093\/jxb\/err031","article-title":"Salicylic acid beyond defence: Its role in plant growth and development","volume":"62","author":"Plasencia","year":"2011","journal-title":"J. Exp. Bot."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.pbi.2007.08.008","article-title":"Salicylic acid in plant defense\u2014The players and protagonists","volume":"10","author":"Loake","year":"2007","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.pbi.2019.02.004","article-title":"Salicylic acid: Biosynthesis, perception, and contributions to plant immunity","volume":"50","author":"Zhang","year":"2019","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gamalero, E., and Glick, B.R. (2012). Ethylene and abiotic stress tolerance in plants. Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change, Springer.","DOI":"10.1007\/978-1-4614-0815-4_18"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1093\/aob\/mct067","article-title":"Jasmonates: Biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in annals of botany","volume":"111","author":"Wasternack","year":"2013","journal-title":"Ann. Bot."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Seyfferth, C., and Tsuda, K. (2014). Salicylic acid signal transduction: The initiation of biosynthesis, perception and transcriptional reprogramming. Front. Plant Sci., 5.","DOI":"10.3389\/fpls.2014.00697"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ding, L.-N., Li, Y.-T., Wu, Y.-Z., Li, T., Geng, R., Cao, J., Zhang, W., and Tan, X.-L. (2022). Plant disease resistance-related signaling pathways: Recent progress and future prospects. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232416200"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.molp.2019.12.008","article-title":"Biosynthesis and regulation of salicylic acid and N-Hydroxypipecolic acid in plant immunity","volume":"13","author":"Huang","year":"2020","journal-title":"Mol. Plant"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e0156","DOI":"10.1199\/tab.0156","article-title":"Salicylic acid biosynthesis and metabolism","volume":"9","author":"Dempsey","year":"2011","journal-title":"Arab. Book"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.pbi.2019.02.006","article-title":"N-Hydroxypipecolic acid and salicylic acid: A metabolic duo for systemic acquired resistance","volume":"50","author":"Hartmann","year":"2019","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_25","first-page":"e4920","article-title":"N-hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis","volume":"115","author":"Chen","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.cell.2018.02.049","article-title":"Flavin monooxygenase-generated N-hydroxypipecolic acid is a critical element of plant systemic immunity","volume":"173","author":"Hartmann","year":"2018","journal-title":"Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"395","DOI":"10.5511\/plantbiotechnology.23.395","article-title":"The phenylalanine pathway is the main route of salicylic acid biosynthesis in tobacco mosaic virus-infected tobacco leaves","volume":"23","author":"Ogawa","year":"2006","journal-title":"Plant Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1104\/pp.103.2.315","article-title":"Pathway of salicylic acid biosynthesis in healthy and virus-inoculated tobacco","volume":"103","author":"Yalpani","year":"1993","journal-title":"Plant Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1104\/pp.110.157370","article-title":"Functional analysis of the Arabidopsis PAL gene family in plant growth, development, and response to environmental stress","volume":"153","author":"Huang","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/nph.14078","article-title":"Cooperative functioning between phenylalanine ammonia lyase and isochorismate synthase activities contributes to salicylic acid biosynthesis in soybean","volume":"212","author":"Shine","year":"2016","journal-title":"New Phytol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1105\/tpc.16.00665","article-title":"Abnormal inflorescence meristem1 functions in salicylic acid biosynthesis to maintain proper reactive oxygen species levels for root meristem activity in rice","volume":"29","author":"Xu","year":"2017","journal-title":"Plant Cell"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, L., Tsuda, K., Sato, M., Cohen, J.D., Katagiri, F., and Glazebrook, J. (2009). Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000301"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1111\/j.1365-313X.2011.04655.x","article-title":"CBP60g and SARD1 play partially redundant critical roles in salicylic acid signaling","volume":"67","author":"Wang","year":"2011","journal-title":"Plant J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18220","DOI":"10.1073\/pnas.1005225107","article-title":"Control of salicylic acid synthesis and systemic acquired resistance by two members of a plant-specific family of transcription factors","volume":"107","author":"Zhang","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"10159","DOI":"10.1038\/ncomms10159","article-title":"ChIP-seq reveals broad roles of SARD1 and CBP60g in regulating plant immunity","volume":"6","author":"Sun","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/nph.14780","article-title":"TGACG-BINDING FACTOR 1 (TGA1) and TGA4 regulate salicylic acid and pipecolic acid biosynthesis by modulating the expression of systemic acquired resistance deficient 1 (SARD1) and calmodulin-binding protein 60g (CBP60g)","volume":"217","author":"Sun","year":"2018","journal-title":"New Phytol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1126\/science.8266079","article-title":"Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid","volume":"262","author":"Chen","year":"1993","journal-title":"Science"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.2307\/3869902","article-title":"Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance","volume":"6","author":"Brisson","year":"1994","journal-title":"Plant Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.pbi.2007.04.020","article-title":"Networks of WRKY transcription factors in defense signaling","volume":"10","author":"Eulgem","year":"2007","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nature11162","article-title":"NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants","volume":"486","author":"Fu","year":"2012","journal-title":"Nature"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.celrep.2012.05.008","article-title":"The Arabidopsis NPR1 protein is a receptor for the plant defense hormone salicylic acid","volume":"1","author":"Wu","year":"2012","journal-title":"Cell Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1105\/TPC.010115","article-title":"Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression","volume":"13","author":"Yu","year":"2001","journal-title":"Plant Cell"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1146\/annurev.pa.25.040185.003435","article-title":"The regulation of hepatic glutathione","volume":"25","author":"Aw","year":"1985","journal-title":"Annu. Rev. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"eaaz0478","DOI":"10.1126\/sciadv.aaz0478","article-title":"The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance","volume":"6","author":"Lim","year":"2020","journal-title":"Sci. Adv."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"eaar4509","DOI":"10.1126\/sciadv.aar4509","article-title":"Pipecolic acid confers systemic immunity by regulating free radicals","volume":"4","author":"Wang","year":"2018","journal-title":"Sci. Adv."},{"key":"ref_46","first-page":"5123","article-title":"Pipecolic acid, an endogenous mediator of defense amplification and priming, is a critical regulator of inducible plant immunity","volume":"24","author":"Bernsdorff","year":"2012","journal-title":"Plant Cell"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1105\/tpc.19.00350","article-title":"The emergence of a mobile signal for systemic acquired resistance","volume":"31","author":"Tian","year":"2019","journal-title":"Plant Cell"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Chevilly, S., Dolz-Edo, L., Morcillo, L., Vilagrosa, A., L\u00f3pez-Nicol\u00e1s, J.M., Yenush, L., and Mulet, J.M. (2021). Identification of distinctive physiological and molecular responses to salt stress among tolerant and sensitive cultivars of broccoli (Brassica oleracea var. Italica). BMC Plant Biol., 21.","DOI":"10.1186\/s12870-021-03263-4"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"10394","DOI":"10.1021\/acs.jafc.1c03421","article-title":"Physiological and molecular characterization of the differential response of broccoli (Brassica oleracea var. Italica) cultivars reveals limiting factors for broccoli tolerance to drought stress","volume":"69","author":"Chevilly","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1007\/s11105-014-0818-x","article-title":"Cloning and characterization of a GmSAGT1 gene whose transcriptional expression is associated with the level of resistance to Soybean Downy Mildew","volume":"33","author":"Sun","year":"2015","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1104\/pp.108.2.633","article-title":"Salicylic acid in rice (biosynthesis, conjugation, and possible role)","volume":"108","author":"Silverman","year":"1995","journal-title":"Plant Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Dong, H., Shi, S., Zhang, C., Zhu, S., Li, M., Tan, J., Yu, Y., Lin, L., Jia, S., and Wang, X. (2018). Transcriptomic analysis of genes in soybean in response to Peronospora manshurica infection. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4741-7"},{"key":"ref_53","first-page":"187","article-title":"Breeding and cultivation techniques of soybean new variety Fengshou 26","volume":"12","author":"Yang","year":"2009","journal-title":"Bull. Agric. Sci. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"384","DOI":"10.2135\/cropsci1984.0011183X002400020050x","article-title":"Registration of Platte soybean","volume":"24","author":"Williams","year":"1984","journal-title":"Crop Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tolbert, N.E. (1980). Photorespiration, The Biochemistry of Plants, Academic Press. [2nd ed.].","DOI":"10.1016\/B978-0-12-675402-5.50018-0"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1515\/BC.2000.084","article-title":"The structural properties of plant peroxisomes and their metabolic significance","volume":"381","author":"Reumann","year":"2000","journal-title":"Biol. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"527","DOI":"10.18388\/abp.2005_3468","article-title":"Some structural properties of plant serine: Glyoxylate aminotransferase","volume":"52","author":"Truszkiewicz","year":"2005","journal-title":"Acta Biochim. Pol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1105\/tpc.016352","article-title":"Plant eR genes that encode photorespiratory enzymes confer resistance against disease","volume":"16","author":"Taler","year":"2004","journal-title":"Plant Cell"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e27700","DOI":"10.4161\/psb.27700","article-title":"WRKY transcription factors: Jack of many trades in plants","volume":"9","author":"Bakshi","year":"2014","journal-title":"Plant Signal. Behav."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"van Verk, M.C., Bol, J.F., and Linthorst, H.J. (2011). WRKY transcription factors involved in activation of SA biosynthesis genes. BMC Plant Biol., 11.","DOI":"10.1186\/1471-2229-11-89"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1093\/dnares\/dsr033","article-title":"RNASeq analysis of a soybean near-isogenic line carrying bacterial leaf pustule-resistant and -susceptible alleles","volume":"18","author":"Kim","year":"2011","journal-title":"DNA Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"197","DOI":"10.9787\/PBB.2015.3.3.197","article-title":"Genome-wide RNA-seq analysis of differentially expressed transcription factor genes against bacterial leaf pustule in soybean","volume":"3","author":"Kim","year":"2015","journal-title":"Plant Breed. Biotech."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Lin, F., Zhao, M., Baumann, D.D., Ping, J., Sun, L., Liu, Y., Zhang, B., Tang, Z., Hughes, E., and Doerge, R.W. (2014). Molecular response to the pathogen Phytophthora sojae among ten soybean near isogenic lines revealed by comparative transcriptomics. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-18"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Bencke-Malato, M., Cabreira, C., Wiebke-Strohm, B., B\u00fccker-Neto, L., Mancini, E., Osorio, M.B., Homrich, M.S., Turchetto-Zolet, A.C., De Carvalho, M.C., and Stolf, R. (2014). Genome-wide annotation of the soybean WRKY family and functional characterization of gene involved in response to Phakopsora pachyrhizi infection. BMC Plant Biol., 14.","DOI":"10.1186\/s12870-014-0236-0"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1016\/j.ygeno.2018.09.014","article-title":"Transcriptome analysis of soybean WRKY TFs in response to Peronospora manshurica infection","volume":"111","author":"Dong","year":"2019","journal-title":"Genomics"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Jain, S., Chittem, K., Brueggeman, R., Osorno, J.M., Richards, J., and Nelson, B.D. (2016). Comparative transcriptome analysis of resistant and susceptible common bean genotypes in response to soybean cyst nematode infection. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0159338"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/22\/11879\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:26:40Z","timestamp":1760113600000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/22\/11879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,5]]},"references-count":66,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["ijms252211879"],"URL":"https:\/\/doi.org\/10.3390\/ijms252211879","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,11,5]]}}}