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We developed a novel protocol to isolate and culture endothelial progenitor cells (EPCs) and ECs directly from MM patient bone marrow, demonstrating their ability to promote myeloma cell proliferation. Validation studies confirmed that these MM-derived ECs exhibit angiogenic potential as well as the expression of characteristic endothelial lineage markers. These findings underscore the critical role of bone marrow ECs in the MM tumor microenvironment and highlight potential new therapeutic targets to disrupt MM progression.<\/jats:p>","DOI":"10.3390\/ijms252212047","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T03:52:07Z","timestamp":1731297127000},"page":"12047","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization and Experimental Use of Multiple Myeloma Bone Marrow Endothelial Cells and Progenitors"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5548-1564","authenticated-orcid":false,"given":"Filip","family":"Garbicz","sequence":"first","affiliation":[{"name":"Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA"},{"name":"Department of Pathology, Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA 02115, USA"},{"name":"Department of Experimental 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