{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T03:05:02Z","timestamp":1763348702289,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T00:00:00Z","timestamp":1731283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa de Minas Gerais (FAPEMIG)","award":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"],"award-info":[{"award-number":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"]}]},{"name":"National Council for Scientific and Technological Development","award":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"],"award-info":[{"award-number":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"],"award-info":[{"award-number":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"]}]},{"name":"Pr\u00f3-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPQ)","award":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"],"award-info":[{"award-number":["APQ-02266-18","RED-0096-22","446409\/2014-6","308877\/2018-7","317732\/2023-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Leishmania spp. commonly infects phagocytic cells of the immune system, particularly macrophages, employing various immune evasion strategies that enable their survival by altering the intracellular environment. In mammals, these parasites establish persistent infections by modulating gene expression in macrophages, thus interfering with immune signaling and response pathways, ultimately creating a favorable environment for the parasite\u2019s survival and reproduction. In this study, our objective was to use data mining and subsequent filtering techniques to identify the genes that play a crucial role in the infection process of Leishmania spp. We aimed to pinpoint genes that have the potential to influence the progression of Leishmania infection. To achieve this, we exploited prior, curated knowledge from major databases and constructed 16 datasets of human molecular information consisting of coding genes and corresponding proteins. We obtained over 400 proteins, identifying approximately 200 genes. The proteins coded by these genes were subsequently used to build a network of protein\u2013protein interactions, which enabled the identification of key players; we named this set Predicted Genes. Then, we selected approximately 10% of Predicted Genes for biological validation. THP-1 cells, a line of human macrophages, were infected with Leishmania\u00a0major in vitro for the validation process. We observed that L. major has the capacity to impact crucial genes involved in the immune response, resulting in macrophage inactivation and creating a conducive environment for the survival of Leishmania parasites.<\/jats:p>","DOI":"10.3390\/ijms252212084","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T03:52:07Z","timestamp":1731297127000},"page":"12084","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression of Network Medicine-Predicted Genes in Human Macrophages Infected with Leishmania major"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6351-3327","authenticated-orcid":false,"given":"Felipe","family":"Caixeta","sequence":"first","affiliation":[{"name":"Programa Interunidades de P\u00f3s-Gradua\u00e7\u00e3o em Bioinform\u00e1tica, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9994-5212","authenticated-orcid":false,"given":"Vinicius Dantas","family":"Martins","sequence":"additional","affiliation":[{"name":"Cedars Sinai, Biomedical Sciences, Los Angeles, CA 90048, USA"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Braga","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Instituto Israelita de Ensino e Pesquisa, Hospital Israelita Albert Einstein, S\u00e3o Paulo 05652-000, SP, Brazil"},{"name":"Departamento de Biologia, Instituto de Ci\u00eancias Biol\u00f3gicas e Exatas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Carlos Crocco","family":"Afonso","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Instituto de Ci\u00eancias Biol\u00f3gicas e Exatas, Universidade Federal de Ouro Preto, Ouro Preto 35402-136, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3635-7664","authenticated-orcid":false,"given":"Paolo","family":"Tieri","sequence":"additional","affiliation":[{"name":"CNR\u2014Consiglio Nazionale delle Ricerche, IAC Istituto per le Applicazioni del Calcolo, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1442-3552","authenticated-orcid":false,"given":"Filippo","family":"Castiglione","sequence":"additional","affiliation":[{"name":"CNR\u2014Consiglio Nazionale delle Ricerche, IAC Istituto per le Applicazioni del Calcolo, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4327-2922","authenticated-orcid":false,"given":"Leandro Martins","family":"de Freitas","sequence":"additional","affiliation":[{"name":"N\u00facleo de Biointegra\u00e7\u00e3o, Instituto Multidisciplinar em Sa\u00fade, Universidade Federal da Bahia, Vit\u00f3riada Conquista 45029-094, BA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiani Uceli","family":"Maioli","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"},{"name":"Departamento de Nutri\u00e7\u00e3o, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"750","DOI":"10.12688\/f1000research.11120.1","article-title":"Leishmaniasis: A review","volume":"6","author":"Arenas","year":"2017","journal-title":"F1000Research"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.apjtm.2016.06.021","article-title":"Worldwide risk factors in leishmaniasis","volume":"9","author":"Oryan","year":"2016","journal-title":"Asian Pac. 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