{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:30:41Z","timestamp":1790659841360,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T00:00:00Z","timestamp":1731283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre in Poland","award":["52\/PB\/RID\/2023"],"award-info":[{"award-number":["52\/PB\/RID\/2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>For several decades, there has been growing interest in the influence of low-frequency magnetic fields (LFMFs) and red LED light on the healing process. Keratinocytes are cells that play a significant role in the process of wound healing and tissue regeneration. A human keratinocyte cell line (HaCaT) was exposed to an LFMF with low induction (180\u2013195 Hz; 60 \u00b5T, magnetostimulation), red LED light (630 nm; 300 mW, LED therapy), and their combined action (magneto-LED therapy) in in vitro culture conditions. On day 4 and 8 of the experiment, the following parameters were determined: adhesion\/proliferation, adenylate kinase (AK), nitric oxide (NO), cytokines (IL-1\u03b2, IL-6, IL-8, IL-10, IL-12p70, TNF-\u03b1), metalloproteinases (MMP-2, MMP-9), and collagen IV. It was shown that magnetostimulation caused an increase in keratinocyte adhesion\/proliferation and IL-8 secretion and a decrease in IL-12 secretion. The LED therapy resulted in a transient increase in the secretion of NO and cytokines IL-1, IL-12, and IL-6 in keratinocytes. The use of magneto-LED therapy resulted in an increase in keratinocyte adhesion\/proliferation, the secretion of cytokines IL-6 and IL-8, and NO with a simultaneous decrease in MMP-9 secretion. The results of our studies showed that the action of an LFMF with low-induction and LED light on keratinocytes can modulate the biological activity of keratinocytes towards improving the skin healing process.<\/jats:p>","DOI":"10.3390\/ijms252212099","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T08:01:27Z","timestamp":1731312087000},"page":"12099","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Effect of Low-Frequency Magnetic Fields with Low Induction and Red LED Light on Keratinocyte Biological Activity\u2014An In Vitro Research Model"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5694-8253","authenticated-orcid":false,"given":"Renata","family":"Wo\u017aniacka","sequence":"first","affiliation":[{"name":"Institute of Applied Sciences, Academy of Physical Education, 31-001 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Lechowska-Liszka","sequence":"additional","affiliation":[{"name":"Institute of Applied Sciences, Academy of Physical Education, 31-001 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Stenka","sequence":"additional","affiliation":[{"name":"Department of Applied Cosmetology, University of Physical Education and Sport, 80-309 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-8740","authenticated-orcid":false,"given":"Aneta","family":"Bac","sequence":"additional","affiliation":[{"name":"Institute of Applied Sciences, Academy of Physical Education, 31-001 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Homa","sequence":"additional","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Jagiellonian University, 31-001 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Chadzi\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Jagiellonian University, 31-001 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"\u015acis\u0142owska-Czarnecka","sequence":"additional","affiliation":[{"name":"Institute of Applied Sciences, Academy of Physical Education, 31-001 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/s41580-024-00715-1","article-title":"Cellular and molecular mechanisms of skin wound healing","volume":"25","author":"Martin","year":"2024","journal-title":"Nat. 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