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N 0000669"],"award-info":[{"award-number":["Prot. N 0000669"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["PI20\/00114"],"award-info":[{"award-number":["PI20\/00114"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["PI23\/00455"],"award-info":[{"award-number":["PI23\/00455"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["ACIF\/2020\/366"],"award-info":[{"award-number":["ACIF\/2020\/366"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["CIACIF\/2022\/421"],"award-info":[{"award-number":["CIACIF\/2022\/421"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["CIACIF\/2022\/422"],"award-info":[{"award-number":["CIACIF\/2022\/422"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["PCI2021-122099-2B"],"award-info":[{"award-number":["PCI2021-122099-2B"]}]},{"name":"Marie-Sklodowska Curie grant (European Union)","award":["Prot. N 0000669"],"award-info":[{"award-number":["Prot. N 0000669"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["PI20\/00114"],"award-info":[{"award-number":["PI20\/00114"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["PI23\/00455"],"award-info":[{"award-number":["PI23\/00455"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["ACIF\/2020\/366"],"award-info":[{"award-number":["ACIF\/2020\/366"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["CIACIF\/2022\/421"],"award-info":[{"award-number":["CIACIF\/2022\/421"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["CIACIF\/2022\/422"],"award-info":[{"award-number":["CIACIF\/2022\/422"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["PCI2021-122099-2B"],"award-info":[{"award-number":["PCI2021-122099-2B"]}]},{"name":"Department for Life Quality Studies of University of Bologna, Marco Polo","award":["Prot. N 0000669"],"award-info":[{"award-number":["Prot. N 0000669"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Several neurodegenerative diseases (NDDs), such as Huntington\u2019s disease, six of the spinocerebellar ataxias, dentatorubral-pallidoluysian atrophy, and spinobulbar muscular atrophy, are caused by abnormally long polyglutamine (polyQ) tracts. Natural compounds capable of alleviating polyQ-induced toxicity are currently of great interest. In this work, we investigated the modulatory effect against polyQ neurotoxic aggregates exerted by erucin (ERN), an isothiocyanate naturally present in its precursor glucoerucin in rocket salad leaves and in its oxidized form, sulforaphane (SFN), in broccoli. Using C. elegans models expressing polyQ in different tissues, we demonstrated that ERN protects against polyQ-induced toxicity and that its action depends on the catalytic subunit of AMP-activated protein kinase (aak-2\/AMPK\u03b12) and, downstream in this pathway, on the daf-16\/FOXO transcription factor, since nematodes deficient in aak-2\/AMPK\u03b12 and daf-16 did not respond to the treatment, respectively. Although triggered by a different source of neurotoxicity than polyQ diseases, i.e., by \u03b1-synuclein (\u03b1-syn) aggregates, Parkinson\u2019s disease (PD) was also considered in our study. Our results showed that ERN reduces \u03b1-syn aggregates and slightly improves the motility of worms. Therefore, further preclinical studies in mouse models of protein aggregation are justified and could provide insights into testing whether ERN could be a potential neuroprotective compound in humans.<\/jats:p>","DOI":"10.3390\/ijms252212220","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T04:15:49Z","timestamp":1731557749000},"page":"12220","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Erucin, a Natural Isothiocyanate, Prevents Polyglutamine-Induced Toxicity in Caenorhabditis elegans via aak-2\/AMPK and daf-16\/FOXO Signaling"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9657-6340","authenticated-orcid":false,"given":"Martina","family":"Balducci","sequence":"first","affiliation":[{"name":"Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Tortajada","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular, Cellular and Genomic Biomedicine, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46012 Valencia, Spain"},{"name":"Joint Unit for Rare Diseases IIS La Fe-CIPF, 46012 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Trujillo","family":"del R\u00edo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular, Cellular and Genomic Biomedicine, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46012 Valencia, Spain"},{"name":"Joint Unit for Rare Diseases IIS La Fe-CIPF, 46012 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar Collado","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular, Cellular and Genomic Biomedicine, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46012 Valencia, Spain"},{"name":"Joint Unit for Rare Diseases IIS La Fe-CIPF, 46012 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2823-2869","authenticated-orcid":false,"given":"Andrea del Valle","family":"Carranza","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular, Cellular and Genomic Biomedicine, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46012 Valencia, Spain"},{"name":"Joint Unit for Rare Diseases IIS La Fe-CIPF, 46012 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4391-5932","authenticated-orcid":false,"given":"Ana Pilar","family":"Gomez Escribano","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular, Cellular and Genomic Biomedicine, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46012 Valencia, Spain"},{"name":"Joint Unit for Rare Diseases IIS La Fe-CIPF, 46012 Valencia, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1594-0033","authenticated-orcid":false,"given":"Rafael P.","family":"V\u00e1zquez-Manrique","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular, Cellular and Genomic Biomedicine, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46012 Valencia, Spain"},{"name":"Joint Unit for Rare Diseases IIS La Fe-CIPF, 46012 Valencia, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-8575","authenticated-orcid":false,"given":"Andrea","family":"Tarozzi","sequence":"additional","affiliation":[{"name":"Department for Life Quality Studies, University of Bologna, 47921 Rimini, Italy"},{"name":"Biostructures and Biosystems National Institute (INBB), 00136 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/B978-0-444-63233-3.00009-9","article-title":"Repeat expansion diseases","volume":"147","author":"Paulson","year":"2018","journal-title":"Handb. 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