{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:36:19Z","timestamp":1760146579612,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Brazilian Council for Scientific and Technological Development (CNPq)","doi-asserted-by":"publisher","award":["404181\/2012-0","06200151\/2020","01.02.016301.03393\/2021-80","01.02.016301.01090\/2023-94","002\/2023-POSGRAD"],"award-info":[{"award-number":["404181\/2012-0","06200151\/2020","01.02.016301.03393\/2021-80","01.02.016301.01090\/2023-94","002\/2023-POSGRAD"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)","award":["404181\/2012-0","06200151\/2020","01.02.016301.03393\/2021-80","01.02.016301.01090\/2023-94","002\/2023-POSGRAD"],"award-info":[{"award-number":["404181\/2012-0","06200151\/2020","01.02.016301.03393\/2021-80","01.02.016301.01090\/2023-94","002\/2023-POSGRAD"]}]},{"name":"FAPEAM RESOLUC\u0327\u00c3O N.","award":["404181\/2012-0","06200151\/2020","01.02.016301.03393\/2021-80","01.02.016301.01090\/2023-94","002\/2023-POSGRAD"],"award-info":[{"award-number":["404181\/2012-0","06200151\/2020","01.02.016301.03393\/2021-80","01.02.016301.01090\/2023-94","002\/2023-POSGRAD"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Leishmaniasis, a disease caused by protozoan Leishmania spp., exhibits a broad range of clinical manifestations. Host resistance or susceptibility to infections is often influenced by the genetic make-up associated with natural immunity. Caspase-1, a key component of the NLRP3 inflammasome, is critical for processing pro-IL-1\u03b2 into its active form, IL-1\u03b2, while CARD8 functions as an NLRP3 inflammasome inhibitor. We conducted a case\u2013control study comparing L. guyanensis-cutaneous leishmaniasis (Lg-CL) patients with healthy individuals (HCs) by analyzing the CASP1 genetic variants rs530537A&gt;G, rs531542C&gt;T, rs531604A&gt;T and rs560880G&gt;T. Additionally, a combined analysis of CARD8rs2043211A&gt;T with CASP1rs530537 was performed. The genotype distribution for the four variants showed no significant differences between Lg-CL patients and HCs. However, the haplotype analysis of the four CASP1 variants identified the GTTT haplotype as associated with a 19% decreased likelihood of Lg-CL development, suggesting a protective effect against disease progression. The combined analysis of CARD8 with CASP1 variants indicated that individuals homozygous for both variants (GG\/TT) exhibited a 38% reduced risk of developing Lg-CL (OR = 0.62 [95%CI:0.46\u20130.83]) in comparison to individuals with other genotype combinations. No correlation was found between the CASP1 variant genotypes and plasma IL-1\u03b2 levels. CASP1 may act as a genetic modifier in Lg-CL.<\/jats:p>","DOI":"10.3390\/ijms252212438","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T11:58:07Z","timestamp":1732017487000},"page":"12438","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Caspase-1 Variants and Plasma IL-1\u03b2 in Patients with Leishmania guyanensis Cutaneous Leishmaniasis in the Amazonas"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0596-8010","authenticated-orcid":false,"given":"Josu\u00e9 Lacerda","family":"de Souza","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Amazonia Legal (Rede Bionorte), Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"},{"name":"Faculdade de Medicina, Universidade Nilton Lins, Manaus 69058030, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus Vinitius","family":"de Farias Guerra","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus 69040000, Amazonas, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5896-1005","authenticated-orcid":false,"given":"Tirza Gabrielle Ramos","family":"de Mesquita","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7922-6090","authenticated-orcid":false,"given":"Jos\u00e9 do Esp\u00edrito Santo","family":"Junior","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Nilton Lins, Manaus 69058030, Amazonas, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada, Universidade Federal do Amazonas, Manaus 69060001, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector David Graterol","family":"Sequera","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lener Santos","family":"da Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Amazonia Legal (Rede Bionorte), Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"},{"name":"Faculdade de Medicina, Universidade Nilton Lins, Manaus 69058030, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Almeida","family":"da Silva","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus 69040000, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipe Menezes","family":"Moura","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizandra Stephanny Fernandes","family":"Menescal","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada, Universidade Federal do Amazonas, Manaus 69060001, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00falia","family":"da Costa Torres","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus 69040000, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1132-039X","authenticated-orcid":false,"given":"Suzana Kanawati","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herllon Karllos Athaydes","family":"Kerr","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio Morishi","family":"Ogusku","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micobacteriologia, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69060001, Amazonas, Brazil"},{"name":"Genomic Health Surveillance Network: Optimization of Assistance and Research in The State of Amazonas\u2014REGESAM, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4273-2678","authenticated-orcid":false,"given":"Mara L\u00facia Gomes","family":"de Souza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado, Manaus 69040000, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2177-7292","authenticated-orcid":false,"given":"Jose Pereira","family":"de Moura Neto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada, Universidade Federal do Amazonas, Manaus 69060001, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aya","family":"Sadahiro","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada, Universidade Federal do Amazonas, Manaus 69060001, Amazonas, Brazil"},{"name":"Genomic Health Surveillance Network: Optimization of Assistance and Research in The State of Amazonas\u2014REGESAM, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendranath","family":"Ramasawmy","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia da Amazonia Legal (Rede Bionorte), Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"},{"name":"Faculdade de Medicina, Universidade Nilton Lins, Manaus 69058030, Amazonas, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69055038, Amazonas, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Imunologia B\u00e1sica e Aplicada, Universidade Federal do Amazonas, Manaus 69060001, Amazonas, Brazil"},{"name":"Genomic Health Surveillance Network: Optimization of Assistance and Research in The State of Amazonas\u2014REGESAM, Manaus 69055038, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"unstructured":"World Health Organization (2023). 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