{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:00:56Z","timestamp":1780628456374,"version":"3.54.1"},"reference-count":88,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ISCIII\u2014Instituto de Salud Carlos III","award":["PI21\/00896"],"award-info":[{"award-number":["PI21\/00896"]}]},{"name":"ISCIII\u2014Instituto de Salud Carlos III","award":["CP23\/00017"],"award-info":[{"award-number":["CP23\/00017"]}]},{"name":"Miguel Servet Program","award":["PI21\/00896"],"award-info":[{"award-number":["PI21\/00896"]}]},{"name":"Miguel Servet Program","award":["CP23\/00017"],"award-info":[{"award-number":["CP23\/00017"]}]},{"name":"CIBER de Enfermedades Respiratorias (CIBERES), ISCIII","award":["PI21\/00896"],"award-info":[{"award-number":["PI21\/00896"]}]},{"name":"CIBER de Enfermedades Respiratorias (CIBERES), ISCIII","award":["CP23\/00017"],"award-info":[{"award-number":["CP23\/00017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Asthma is a chronic inflammatory lung disease with high prevalence, making it one of the most common chronic conditions worldwide. Its pathophysiology is influenced by a range of genetic and environmental factors, resulting in a complex and heterogeneous disease profile. Asthma is primarily associated with a type 2 (T2) immune response, though non-T2 endotypes also contribute to disease pathology. Generally, asthma is characterized by the infiltration and activation of various cell types, including dendritic cells, eosinophils, innate lymphoid cells, lymphocytes, mast cells, and neutrophils, which participate in T1, T2, and T17 immune responses. Despite advances in understanding, many questions remain unresolved. Therefore, emerging omic techniques, such as single-cell RNA sequencing (scRNA-seq), offer novel insights into the underlying mechanisms of asthma and the roles of these immune cells. Recent scRNA-seq studies in asthma have identified multiple novel immune cell subtypes and clusters, suggesting their potential functions in disease pathology. The rapid advancement of scRNA-seq technology now enables in-depth investigation of individual cells within tissues, allowing for precise cell-type classification and detailed molecular profiling. Nonetheless, certain limitations persist, which require further refinement in future studies.<\/jats:p>","DOI":"10.3390\/ijms252312633","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T03:59:01Z","timestamp":1732507141000},"page":"12633","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single-Cell Analysis: A Method for In-Depth Phenotyping of Cells Involved in Asthma"],"prefix":"10.3390","volume":"25","author":[{"given":"Daniel","family":"Rodr\u00edguez-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Fundaci\u00f3n Jim\u00e9nez D\u00edaz (IIS-FJD),  Universidad Aut\u00f3noma de Madrid (UAM), 28040 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Guill\u00e9n-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Fundaci\u00f3n Jim\u00e9nez D\u00edaz (IIS-FJD),  Universidad Aut\u00f3noma de Madrid (UAM), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6228-1969","authenticated-orcid":false,"given":"Victoria","family":"del Pozo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Fundaci\u00f3n Jim\u00e9nez D\u00edaz (IIS-FJD),  Universidad Aut\u00f3noma de Madrid (UAM), 28040 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain"},{"name":"Medicine Department, School of Medicine, Faculty of Medicine, Campus of Medicine, Universidad Aut\u00f3noma de Madrid (UAM), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0460-2813","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"Ca\u00f1as","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Fundaci\u00f3n Jim\u00e9nez D\u00edaz (IIS-FJD),  Universidad Aut\u00f3noma de Madrid (UAM), 28040 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red (CIBER) de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.cell.2021.02.016","article-title":"The basic immunology of asthma","volume":"184","author":"Hammad","year":"2021","journal-title":"Cell"},{"key":"ref_2","first-page":"118","article-title":"Understanding the immunology of asthma: Pathophysiology, biomarkers, and treatments for asthma endotypes","volume":"36","author":"Gans","year":"2020","journal-title":"Paediatr. 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