{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:49:11Z","timestamp":1787028551235,"version":"build-2736575974"},"reference-count":68,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic kidney disease is a really important heath issue, and transplantation is an intervention that can greatly increase patient quality of life and survival. The aim of this study was to perform a comprehensive evaluation of the BK virus, CMV, and EBV in kidney transplant recipients (KTRs); to assess the prevalence of infections; and to test if our detection method would be feasible for use in follow-ups with KTRs. A total of 157 KTRs registered at the Clinical Hospital \u201cDr. C. I. Parhon\u201d, Ia\u0219i, Romania, were selected using specific inclusion\/exclusion criteria. We tested the blood samples from each patient for BK, EBV, and CMV using a multiplex real-time PCR (qPCR) assay and the TaqMan PCR principle. The highest prevalence was detected for BKV (11\/157, 7%), followed by CMV (9\/157, 5.7%) and EBV (5\/157, 3.2%). By simultaneously detecting three possible nephropathic viruses and oncogenes in KTRs using multiplex real-time PCR, we aimed to optimize their monitoring and follow-up. The prevalence of the tested nephropathogenic viruses\u2014BKV, CMV, and EBV\u2014was comparable to that analyzed in other studies. We demonstrate that the use of qPCR for viral detection in KTRs is a robust, cost-effective method for case monitoring.<\/jats:p>","DOI":"10.3390\/ijms252312698","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T07:40:32Z","timestamp":1732606832000},"page":"12698","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Accurate Multiplex qPCR Detection of Epstein\u2013Barr Virus\/Cytomegalovirus\/BK Virus in Kidney Transplant Patients: Pilot Study"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-3398","authenticated-orcid":false,"given":"Costin","family":"Damian","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1811-7923","authenticated-orcid":false,"given":"Ramona Gabriela","family":"Ursu","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9985-2486","authenticated-orcid":false,"given":"Adrian Constantin","family":"Covic","sequence":"additional","affiliation":[{"name":"Nephrology Department, Dialysis and Renal Transplant Center, \u201cDr. C.I. Parhon\u201d University Hospital, 700503 Iasi, Romania"},{"name":"Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida Corina","family":"B\u0103descu","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona Mihaela","family":"Hoga\u0219","sequence":"additional","affiliation":[{"name":"Nephrology Department, Dialysis and Renal Transplant Center, \u201cDr. C.I. Parhon\u201d University Hospital, 700503 Iasi, Romania"},{"name":"Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0126-0640","authenticated-orcid":false,"given":"Elena Roxana","family":"Buzil\u0103","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6076-7818","authenticated-orcid":false,"given":"Alexandru","family":"Duhaniuc","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2592-3982","authenticated-orcid":false,"given":"Lumini\u021ba Smaranda","family":"Iancu","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Faculty of Medicine, \u201cGrigore T. Popa\u201d University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2316277","DOI":"10.1080\/0886022X.2024.2316277","article-title":"Global Trends of Delayed Graft Function in Kidney Transplantation from 2013 to 2023: A Bibliometric Analysis","volume":"46","author":"Yao","year":"2024","journal-title":"Ren Fail"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e382","DOI":"10.1016\/S2214-109X(23)00570-3","article-title":"An Update on the Global Disparities in Kidney Disease Burden and Care across World Countries and Regions","volume":"12","author":"Bello","year":"2024","journal-title":"Lancet Glob. Health"},{"key":"ref_3","unstructured":"(2024, October 01). Nefrovisie Nierfunctievervanging Nederland\u2014Jaarboek 2019 (2020) 2020. 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