{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T05:02:59Z","timestamp":1784264579975,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Culture, Sports, Science, and Technology (Japan)","award":["22KJ1244"],"award-info":[{"award-number":["22KJ1244"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although hyperplasia of the anorectal transitional zone (TZ) has been reported in mouse models of ulcerative colitis, the mechanisms underlying this phenomenon are not fully understood. We characterized keratin subtypes and examined the expression of stem cell markers in the TZ. Dextran sodium sulfate-treated mice showed abnormal repair of the anorectal region, which consisted of mixed hyperplastic TZ and regenerating crypts. Liquid chromatography-tandem mass spectrometry from the paraffin-embedded TZ in the treated mice revealed that the major keratins were type I cytokeratin (CK)13 and type II CK5, but notable expression of type I CK10 and CK42 and type II CK1, CK4, CK6a, CK8, and CK15 was also detected. Hyperplastic TZ was characterized by the expression of tumor protein 63, sex-determining region Y-box 2 (SOX2), SOX9, and leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5). Lgr5 was highly expressed in the TZ in the early stages of colitis, followed by higher expression levels of SOX2. The TZ-derived organoids expressed LGR5, SOX2, and SOX9. The present study suggests that transitional zones showing abnormal keratin assembly and stem cell activation may interfere with rectal crypt regeneration, leading to pathological anorectal remodeling in severe colitis.<\/jats:p>","DOI":"10.3390\/ijms252312706","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T09:10:14Z","timestamp":1732612214000},"page":"12706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anorectal Remodeling in the Transitional Zone with Increased Expression of LGR5, SOX9, SOX2, and Keratin 13 and 5 in a Dextran Sodium Sulfate-Induced Mouse Model of Ulcerative Colitis"],"prefix":"10.3390","volume":"25","author":[{"given":"Mio","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Pathology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan"},{"name":"Cooperative Division of Veterinary Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Usui","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9368-1535","authenticated-orcid":false,"given":"Mohamed","family":"Elbadawy","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan"},{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Elqaliobiya, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2219-9634","authenticated-orcid":false,"given":"Tetsuhito","family":"Kigata","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0660-7156","authenticated-orcid":false,"given":"Masahiro","family":"Kaneda","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, 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Ophthalmol. Vis. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/nature24269","article-title":"Transitional basal cells at the squamous-columnar junction generate Barrett\u2019s oesophagus","volume":"550","author":"Jiang","year":"2017","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1038\/modpathol.2015.54","article-title":"Microanatomy of the cervical and anorectal squamocolumnar junctions: A proposed model for anatomical differences in HPV-related cancer risk","volume":"28","author":"Yang","year":"2015","journal-title":"Mod. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6330","DOI":"10.1002\/cam4.3201","article-title":"Switches of SOX17 and SOX2 expression in the development of squamous metaplasia and squamous intraepithelial lesions of the uterine cervix","volume":"9","author":"Moshi","year":"2020","journal-title":"Cancer Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3039","DOI":"10.4161\/cc.9.15.12437","article-title":"Identification of epithelial label-retaining cells at the transition between the anal canal and the rectum in mice","volume":"9","author":"Runck","year":"2010","journal-title":"Cell Cycle"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.1038\/s41467-021-23034-x","article-title":"A stem cell population at the anorectal junction maintains homeostasis and participates in tissue regeneration","volume":"12","author":"Mitoyan","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Per\u0161e, M., and Cerar, A. (2012). Dextran sodium sulphate colitis mouse model: Traps and tricks. J. Biomed. Biotechnol., 2012.","DOI":"10.1155\/2012\/718617"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1053\/j.gastro.2022.02.031","article-title":"Transitional Anal Cells Mediate Colonic Re-epithelialization in Colitis","volume":"162","author":"Liu","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1684\/ejd.2011.1267","article-title":"Epithelial transition zones: Merging microenvironments, niches, and cellular transformation","volume":"21","author":"Mcnairn","year":"2011","journal-title":"Eur. J. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1038\/nrc.2017.68","article-title":"Metaplasia: Tissue injury adaptation and a precursor to the dysplasia-cancer sequence","volume":"17","author":"Giroux","year":"2017","journal-title":"Nat. Rev. Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1053\/j.gastro.2021.09.051","article-title":"Epithelium replacement contributes to field expansion of squamous epithelium and ulcerative colitis-associated nneoplasia","volume":"162","author":"Sugimoto","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.4161\/cc.6.11.4268","article-title":"TGF-beta receptor I conditional knockout mice develop spontaneous squamous cell carcinoma","volume":"6","author":"Honjo","year":"2007","journal-title":"Cell Cycle"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"G1328","DOI":"10.1152\/ajpgi.00467.2004","article-title":"Acute colitis induced by dextran sulfate sodium progresses to chronicity in C57BL\/6 but not in BALB\/c mice: Correlation between symptoms and inflammation","volume":"288","author":"Melgar","year":"2005","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"869","DOI":"10.3892\/or.2010.869","article-title":"Increased apoptosis and decreased proliferation of colonic epithelium in dextran sulfate sodium-induced colitis in mice","volume":"24","author":"Araki","year":"2010","journal-title":"Oncol. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/0016-5085(90)90290-H","article-title":"A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice","volume":"98","author":"Okayasu","year":"1990","journal-title":"Gastroenterology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1046\/j.1440-1746.1999.01806.x","article-title":"Dextran sodium sulphate-induced colitis activity varies with mouse strain but develops in lipopolysaccharide-unresponsive mice","volume":"14","author":"Stevceva","year":"1999","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1538\/expanim.49.9","article-title":"Histological analysis of murine colitis induced by dextran sulfate sodium of different molecular weights","volume":"49","author":"Kitajima","year":"2000","journal-title":"Exp. Anim."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15.25.1","DOI":"10.1002\/0471142735.im1525s104","article-title":"Dextran sulfate sodium (DSS)-induced colitis in mice","volume":"104","author":"Chassaing","year":"2014","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1177\/0023677217742681","article-title":"A refined and translationally relevant model of chronic DSS colitis in BALB\/c mice","volume":"52","author":"Hoffmann","year":"2018","journal-title":"Lab. Anim."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"G10","DOI":"10.1152\/ajpgi.00277.2011","article-title":"Distinct levels of Sox9 expression mark colon epithelial stem cells that form colonoids in culture","volume":"302","author":"Ramalingam","year":"2012","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1053\/j.gastro.2015.07.004","article-title":"SOX9 maintains reserve stem cells and preserves radioresistance in mouse small intestine","volume":"149","author":"Roche","year":"2015","journal-title":"Gastroenterology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1242\/dev.038620","article-title":"Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis","volume":"136","author":"Driskell","year":"2009","journal-title":"Development"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ydbio.2013.08.004","article-title":"Sox2 modulates the function of two distinct cell lineages in mouse skin","volume":"382","author":"Lesko","year":"2013","journal-title":"Dev. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"52085","DOI":"10.18632\/oncotarget.10475","article-title":"Lgr5+ stem cells and their progeny in mouse epidermis under regimens of exogenous skin carcinogenesis, and their absence in ensuing skin tumors","volume":"7","author":"Rebel","year":"2016","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106919","DOI":"10.1016\/j.isci.2023.106919","article-title":"Sox9 regulates melanocytic fate decision of adult hair follicle stem cells","volume":"26","author":"Beyer","year":"2023","journal-title":"iScience"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/exd.14303","article-title":"Stem and progenitor cells in sebaceous gland development, homeostasis and pathologies","volume":"30","author":"Geueke","year":"2021","journal-title":"Exp. Dermatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4770","DOI":"10.1007\/s10620-021-07340-4","article-title":"Ectopically localized epithelial cell clumps in ulcers are derived from reserved crypt stem cells in a mouse model of ulcerative colitis","volume":"67","author":"Kobayashi","year":"2022","journal-title":"Dig. Dis. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104117","DOI":"10.1016\/j.jprot.2021.104117","article-title":"Comparative evaluation of two methods for LC-MS\/MS proteomic analysis of formalin fixed and paraffin embedded tissues","volume":"235","author":"Davalieva","year":"2021","journal-title":"J. Proteom."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Obi, E.N., Tellock, D.A., Thomas, G.J., and Veenstra, T.D. (2023). Biomarker analysis of formalin-fixed paraffin-embedded clinical tissues using proteomics. Biomolecules, 13.","DOI":"10.3390\/biom13010096"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"105315","DOI":"10.1016\/j.rvsc.2024.105315","article-title":"Systemic AL kappa chain amyloidosis in a captive Bornean orangutan (Pongo pygmaeus)","volume":"175","author":"Iwaide","year":"2024","journal-title":"Res. Vet. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1111\/j.1469-7580.2009.01066.x","article-title":"Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia","volume":"214","author":"Bragulla","year":"2009","journal-title":"J. Anat."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"86","DOI":"10.4103\/jomfp.JOMFP_195_16","article-title":"Pathophysiology of keratinization","volume":"22","author":"Deo","year":"2018","journal-title":"J. Oral Maxillofac. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/j.jid.2019.02.004","article-title":"SOX2 epidermal overexpression promotes cutaneous wound healing via activation of EGFR\/MEK\/ERK signaling mediated by EGFR ligands","volume":"139","author":"Uchiyama","year":"2019","journal-title":"J. Investig. Dermatol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Menzel-Severing, J., Zenkel, M., Polisetti, N., Sock, E., Wegner, M., Kruse, F.E., and Schl\u00f6tzer-Schrehardt, U. (2018). Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem\/progenitor cells. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-28596-3"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1148\/rg.2015150037","article-title":"Anorectal cancer: Critical anatomic and staging distinctions that affect use of radiation therapy","volume":"35","author":"Matalon","year":"2015","journal-title":"Radiographics"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Shi, G., Sohn, K.C., Li, Z., Choi, D.K., Park, Y.M., Kim, J.H., Fan, Y.M., Nam, Y.H., Kim, S., and Im, M. (2013). Expression and functional role of Sox9 in human epidermal keratinocytes. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0054355"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/nature13305","article-title":"SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma","volume":"511","author":"Boumahdi","year":"2014","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.semcancer.2019.03.004","article-title":"The role of SOX family members in solid tumours and metastasis","volume":"67","author":"Grimm","year":"2020","journal-title":"Semin. Cancer Biol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Barbosa, S., Laureano, N.K., Hadiwikarta, W.W., Visioli, F., Bonrouhi, M., Pajdzik, K., Conde-Lopez, C., Herold-Mende, C., Eidt, G., and Langie, R. (2024). The role of SOX2 and SOX9 in radioresistance and tumor recurrence. Cancers, 16.","DOI":"10.3390\/cancers16020439"},{"key":"ref_41","first-page":"11267","article-title":"Expression of LGR5 in oral squamous cell carcinoma and its correlation to vasculogenic mimicry","volume":"10","author":"Wu","year":"2017","journal-title":"Int. J. Clin Exp. Pathol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/s41467-019-13847-2","article-title":"Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5\u2212CD44+ cells","volume":"11","author":"Fu","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1038\/ng.3957","article-title":"Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma","volume":"49","author":"Huang","year":"2017","journal-title":"Nat. Genet."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e1234","DOI":"10.1002\/prp2.1234","article-title":"Estrogen deprivation and estrogen receptor \u03b1 antagonism decrease DSS colitis in female mice","volume":"12","author":"Hjelt","year":"2024","journal-title":"Pharmacol. Res. Perspect."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"G1111","DOI":"10.1152\/ajpgi.00519.2011","article-title":"Activation of two distinct Sox9-EGFP-expressing intestinal stem cell populations during crypt regeneration after irradiation","volume":"302","author":"Santoro","year":"2012","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.etp.2017.04.002","article-title":"Expression of A-kinase anchor protein 13 and Rho-associated coiled-coil containing protein kinase in restituted and regenerated mucosal epithelial cells following mucosal injury and colorectal cancer cells in mouse models","volume":"69","author":"Kangawa","year":"2017","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_47","first-page":"493","article-title":"Suppression of epithelial restitution using an inhibitor against Rho-associated coiled-coil containing protein kinase aggravates colitis through reduced epithelial expression of A-kinase anchor protein 13","volume":"45","author":"Kangawa","year":"2017","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/nmeth741","article-title":"Stem cell proliferative history in tissue revealed by temporal halogenated thymidine analog discrimination","volume":"2","author":"Vega","year":"2005","journal-title":"Nat. Methods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.stemcr.2017.12.020","article-title":"Triple s-phase labeling of dividing stem cells","volume":"10","author":"Podgorny","year":"2018","journal-title":"Stem Cell Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"8484","DOI":"10.1038\/s41467-023-44138-6","article-title":"Adult stem cell activity in naked mole rats for long-term tissue maintenance","volume":"14","author":"Montazid","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1074\/mcp.M500061-MCP200","article-title":"Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein","volume":"4","author":"Ishihama","year":"2005","journal-title":"Mol. Cell Proteomics"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Elbadawy, M., Kato, Y., Saito, N., Hayashi, K., Abugomaa, A., Kobayashi, M., Yoshida, T., Shibutani, M., Kaneda, M., and Yamawaki, H. (2021). Establishment of intestinal organoid from Rousettus leschenaultii and the susceptibility to bat-associated viruses, SARS-CoV-2 and pteropine orthoreovirus. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910763"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/23\/12706\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:39:52Z","timestamp":1760114392000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/23\/12706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,26]]},"references-count":52,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["ijms252312706"],"URL":"https:\/\/doi.org\/10.3390\/ijms252312706","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,26]]}}}