{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:20:29Z","timestamp":1782739229027,"version":"3.54.5"},"reference-count":56,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Sailing Program","award":["21YF1459700"],"award-info":[{"award-number":["21YF1459700"]}]},{"name":"Shanghai Sailing Program","award":["2023XT0205"],"award-info":[{"award-number":["2023XT0205"]}]},{"name":"Shanghai Sailing Program","award":["2023TD31"],"award-info":[{"award-number":["2023TD31"]}]},{"name":"Shanghai Sailing Program","award":["2019M06"],"award-info":[{"award-number":["2019M06"]}]},{"name":"Shanghai Sailing Program","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["21YF1459700"],"award-info":[{"award-number":["21YF1459700"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["2023XT0205"],"award-info":[{"award-number":["2023XT0205"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["2023TD31"],"award-info":[{"award-number":["2023TD31"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["2019M06"],"award-info":[{"award-number":["2019M06"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["21YF1459700"],"award-info":[{"award-number":["21YF1459700"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2023XT0205"],"award-info":[{"award-number":["2023XT0205"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2023TD31"],"award-info":[{"award-number":["2023TD31"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2019M06"],"award-info":[{"award-number":["2019M06"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]},{"name":"China Agriculture Research System","award":["21YF1459700"],"award-info":[{"award-number":["21YF1459700"]}]},{"name":"China Agriculture Research System","award":["2023XT0205"],"award-info":[{"award-number":["2023XT0205"]}]},{"name":"China Agriculture Research System","award":["2023TD31"],"award-info":[{"award-number":["2023TD31"]}]},{"name":"China Agriculture Research System","award":["2019M06"],"award-info":[{"award-number":["2019M06"]}]},{"name":"China Agriculture Research System","award":["CARS-48"],"award-info":[{"award-number":["CARS-48"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The conserved role of juvenile hormone (JH) signals in preventing larvae from precocious metamorphosis has been confirmed in insects. Crustaceans have different metamorphosis types from insects; we previously proved that methyl farnesoate (MF) can prohibit larvae metamorphosis in mud crabs, but the molecular signal of this process still needs to be elucidated. In this study, methoprene-tolerant (Met) of Scylla paramamosain was obtained and characterized, which we named Sp-Met. Sp-Met contains a 3360 bp ORF that encodes 1119 amino acids; the predicted protein sequences of Sp-Met include one bHLH, two PAS domains, one PAC domain, and several long unusual Gln repeats at the C-terminal. AlphaFold2 was used to predict the 3D structure of Sp-Met and the JH binding domain of Met. Furthermore, the binding properties between Sp-Met and MF were analyzed using CD-DOCK2, revealing a putative high affinity between the receptor and ligand. In silico site-directed mutagenesis suggested that insect Mets may have evolved to exhibit a higher affinity for both MF or JH III compared to the Mets of crustaceans. In addition, we found that the expression of Sp-Met was significantly higher in female reproductive tissues than in males but lower in most of the other examined tissues. During larval development, the expression variation in Sp-Met and Sp-Kr-h1 was consistent with the immersion effect of MF. The most interesting finding is that knockdown of Sp-Met blocked the inhibitory effect of MF on metamorphosis in the fifth zoea stage and induced pre-metamorphosis phenotypes in the fourth zoea stage. The knockdown of Sp-Met significantly reduced the expression of Sp-Kr-h1 and two ecdysone signaling genes, Sp-EcR and Sp-E93. However, only the reduction in Sp-Kr-h1 could be rescued by MF treatment. In summary, this study provides the first evidence that MF inhibits crustacean larval metamorphosis through Met and that the MF-Met\u2192Kr-h1 signal pathway is conserved in mud crabs. Additionally, the crosstalk between MF and ecdysteroid signaling may have evolved differently in mud crabs compared to insects.<\/jats:p>","DOI":"10.3390\/ijms252312746","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T07:18:42Z","timestamp":1732691922000},"page":"12746","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab, Scylla paramamosain"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1221-9240","authenticated-orcid":false,"given":"Ming","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"},{"name":"College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghao","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"},{"name":"College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"},{"name":"College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyong","family":"Zhan","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"},{"name":"College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyi","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingbo","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Farhadi, A., Zhao, Q., and Tan, K. (2024). The Molecular Mechanism of Embryonic Development in Decapod Crustaceans. Rev. Aquac.","DOI":"10.1111\/raq.12981"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1163\/15685403-bja10133","article-title":"Effect of Methyl Farnesoate and Farnesoic Acid During 5th Zoea and Megalopa Metamorphosis in The Mud Crab Scylla Paramamosain estampador, 1950 (Decapoda, Brachyura, Portunidae)","volume":"94","author":"Zhao","year":"2021","journal-title":"Crustaceana"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1111\/1744-7917.12614","article-title":"Juvenile hormone signaling\u2014A mini review","volume":"26","author":"Li","year":"2019","journal-title":"Insect Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.tem.2004.11.003","article-title":"Hormonal cross talk in insect development","volume":"16","author":"Dubrovsky","year":"2005","journal-title":"Trends Endocrinol. Metab. TEM"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1126\/science.235.4785.202","article-title":"Identification of a juvenile hormone-like compound in a crustacean","volume":"235","author":"Laufer","year":"1987","journal-title":"Science"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112","DOI":"10.2307\/1542818","article-title":"Effect of Methyl Farnesoate on Late Larval Development and Metamorphosis in the Prawn Macrobrachium rosenbergii (Decapoda, Palaemonidae): A Juvenoid-like Effect?","volume":"195","author":"Abdu","year":"1998","journal-title":"Biol. Bull."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/S0965-1748(00)00062-X","article-title":"Identification of methyl farnesoate in the cypris larva of the barnacle, Balanus amphitrite, and its role as a juvenile hormone","volume":"30","author":"Smith","year":"2000","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/0020-1790(87)90133-8","article-title":"Methyl farnesoate and its role in crustacean reproduction and development","volume":"17","author":"Borst","year":"1987","journal-title":"Insect Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1038\/ncomms2868","article-title":"A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans","volume":"4","author":"Miyakawa","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ibmb.2017.12.007","article-title":"Juvenile hormone-independent function of Kr\u00fcppel homolog 1 in early development of water flea Daphnia pulex","volume":"93","author":"Miyakawa","year":"2018","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.gene.2018.08.046","article-title":"Role of Kruppel homolog 1 (Kr-h1) in methyl farnesoate-mediated vitellogenesis in the swimming crab Portunus trituberculatus","volume":"679","author":"Xie","year":"2018","journal-title":"Gene"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"106653","DOI":"10.1016\/j.anireprosci.2020.106653","article-title":"Effects of methyl farnesoate on Kr\u00fcppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)","volume":"224","author":"Li","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhang, X., Li, S., and Liu, S. (2021). Juvenile Hormone Studies in Drosophila melanogaster. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.785320"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8437","DOI":"10.1074\/jbc.M110.191684","article-title":"Steroid Receptor Co-activator Is Required for Juvenile Hormone Signal Transduction through a bHLH-PAS Transcription Factor, Methoprene Tolerant","volume":"286","author":"Zhang","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mce.2014.06.021","article-title":"A steroid receptor coactivator acts as the DNA-binding partner of the methoprene-tolerant protein in regulating juvenile hormone response genes","volume":"394","author":"Li","year":"2014","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"27874","DOI":"10.1074\/jbc.M114.582825","article-title":"Heat Shock Protein 83 (Hsp83) Facilitates Methoprene-tolerant (Met) Nuclear Import to Modulate Juvenile Hormone Signaling","volume":"289","author":"He","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ibmb.2016.12.005","article-title":"Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met) in an importin \u03b2- and Hsp83-dependent manner","volume":"81","author":"He","year":"2017","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11729","DOI":"10.1073\/pnas.1204951109","article-title":"Transcriptional regulation of juvenile hormone-mediated induction of Kruppel homolog 1, a repressor of insect metamorphosis","volume":"109","author":"Kayukawa","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.ibmb.2011.09.003","article-title":"Drosophila Met and Gce are partially redundant in transducing juvenile hormone action","volume":"41","author":"Abdou","year":"2011","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"27370","DOI":"10.1074\/jbc.M115.687293","article-title":"20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis","volume":"290","author":"Liu","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1074\/jbc.M115.686121","article-title":"Kruppel homolog 1 inhibits insect metamorphosis via direct transcriptional repression of Broad-complex, a pupal specifier gene","volume":"291","author":"Kayukawa","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1073\/pnas.1615423114","article-title":"Molecular mechanism underlying juvenile hormone-mediated repression of precocious larval-adult metamorphosis","volume":"114","author":"Kayukawa","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1073\/pnas.1716897115","article-title":"Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila","volume":"115","author":"Liu","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10488","DOI":"10.1073\/pnas.0703719104","article-title":"Juvenile hormone resistance gene Methoprene-tolerant controls entry into metamorphosis in the beetle Tribolium castaneum","volume":"104","author":"Konopova","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1146\/annurev-ento-120811-153700","article-title":"The Juvenile Hormone Signaling Pathway in Insect Development","volume":"58","author":"Jindra","year":"2013","journal-title":"Annu. Rev. Entomol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/bs.vh.2023.03.002","article-title":"Juvenile hormone receptor Methoprene tolerant: Functions and applications","volume":"123","author":"Palli","year":"2023","journal-title":"Vitam. Horm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"W159","DOI":"10.1093\/nar\/gkac394","article-title":"CB-Dock2: Improved protein\u2013ligand blind docking by integrating cavity detection, docking and homologous template fitting","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"21128","DOI":"10.1073\/pnas.1116123109","article-title":"Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant","volume":"108","author":"Charles","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"R674","DOI":"10.1016\/S0960-9822(06)00352-6","article-title":"PAS: A multifunctional domain family comes to light","volume":"7","author":"Ponting","year":"1997","journal-title":"Curr. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/dgd.12572","article-title":"Juvenile hormone as a physiological regulator mediating phenotypic plasticity in pancrustaceans","volume":"61","author":"Miura","year":"2018","journal-title":"Dev. Growth Differ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.jinsphys.2008.01.014","article-title":"Juvenile hormone action: A 2007 perspective","volume":"54","author":"Riddiford","year":"2008","journal-title":"J. Insect Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"E735","DOI":"10.1073\/pnas.1523838113","article-title":"Hairy and Groucho mediate the action of juvenile hormone receptor Methoprene-tolerant in gene repression","volume":"113","author":"Saha","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","first-page":"E1871","article-title":"Juvenile hormone-activated phospholipase C pathway enhances transcriptional activation by the methoprene-tolerant protein","volume":"112","author":"Liu","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7018","DOI":"10.1073\/pnas.1313058111","article-title":"Juvenile hormone regulates body size and perturbs insulin signaling in Drosophila","volume":"111","author":"Mirth","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"E2173","DOI":"10.1073\/pnas.1305293110","article-title":"Juvenile hormone and its receptor, methoprene-tolerant, control the dynamics of mosquito gene expression","volume":"110","author":"Zou","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Li, X., Chen, T., Han, Y., Huang, M., Jiang, H., Huang, J., Tao, M., Xu, R., Xie, Q., and Su, S. (2021). Potential role of Methoprene-tolerant (Met) in methyl farnesoate-mediated vitellogenesis in the Chinese mitten crab (Eriocheir sinensis). Comp. Biochem. Physiol. Part B Biochem. Mol. Biol., 252.","DOI":"10.1016\/j.cbpb.2020.110524"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1186\/s12964-020-00662-2","article-title":"The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1","volume":"18","author":"Kolonko","year":"2020","journal-title":"Cell Commun. Signal. CCS"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kakaley, E.K.M., Wang, H.Y., and LeBlanc, G.A. (2017). Agonist-mediated assembly of the crustacean methyl farnesoate receptor. Sci. Rep., 7.","DOI":"10.1038\/srep45071"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Zhao, M., Zhang, F., Wang, W., Liu, Z., Ma, C., Fu, Y., Chen, W., and Ma, L. (2022). Identification and Evolution Analysis of the Complete Methyl Farnesoate Biosynthesis and Related Pathway Genes in the Mud Crab, Scylla paramamosain. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23169451"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1086\/719047","article-title":"Molecular Characterization of the Cytochrome P450 Epoxidase (CYP15) in the Swimming Crab Portunus trituberculatus and Its Putative Roles in Methyl Farnesoate Metabolism","volume":"242","author":"Tu","year":"2022","journal-title":"Biol. Bull."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Wen, D., Rivera-Perez, C., Abdou, M., Jia, Q., He, Q., Liu, X., Zyaan, O., Xu, J., Bendena, W.G., and Tobe, S.S. (2015). Methyl Farnesoate Plays a Dual Role in Regulating Drosophila Metamorphosis. PLoS Genet., 11.","DOI":"10.1371\/journal.pgen.1005038"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.anireprosci.2016.09.017","article-title":"Molecular characterization of methoprene-tolerant gene (Met) in the swimming crab Portunus trituberculatus: Its putative role in methyl farnesoate-mediated vitellogenin transcriptional activation","volume":"174","author":"Liu","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Gijbels, M., Lenaerts, C., Broeck, J.V., and Marchal, E. (2019). Juvenile Hormone receptor Met is essential for ovarian maturation in the Desert Locust, Schistocerca gregaria. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-47253-x"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s00359-019-01362-0","article-title":"Mating triggers an up-regulation of vitellogenin and defensin in ant queens","volume":"205","author":"Cherasse","year":"2019","journal-title":"J. Comp. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Toyota, K., Yamane, F., and Ohira, T. (2020). Impacts of Methyl Farnesoate and 20-Hydroxyecdysone on Larval Mortality and Metamorphosis in the Kuruma Prawn Marsupenaeus japonicus. Front. Endocrinol., 11.","DOI":"10.3389\/fendo.2020.00475"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/0309-1651(89)90141-0","article-title":"On the biological function of the mandibular gland of decapod crustacea","volume":"13","author":"Taketomi","year":"1989","journal-title":"Cell Biol. Int. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.aquaculture.2007.04.078","article-title":"A new and practical application of JH antagonist KK-42 to promoting growth of shrimp Penaeus schmitti","volume":"270","author":"Ning","year":"2007","journal-title":"Aquaculture"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Spitzner, F., Meth, R., Kr\u00fcger, C., Nischik, E., Eiler, S., Sombke, A., Torres, G., and Harzsch, S. (2018). An atlas of larval organogenesis in the European shore crab Carcinus maenas L. (Decapoda, Brachyura, Portunidae). Front. Zool., 15.","DOI":"10.1186\/s12983-018-0271-z"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1530\/JOE-14-0526","article-title":"Ecdysone receptor in the mud crab Scylla paramamosain: A possible role in promoting ovarian development","volume":"224","author":"Gong","year":"2015","journal-title":"J. Endocrinol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1242\/dev.033712","article-title":"Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila","volume":"136","author":"Liu","year":"2009","journal-title":"Development"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1111\/1755-0998.13332","article-title":"A chromosome-level genome of the mud crab (Scylla paramamosain Estampador) provides insights into the evolution of chemical and light perception in this crustacean","volume":"21","author":"Zhao","year":"2021","journal-title":"Mol. Ecol. Resour."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1093\/bioinformatics\/btu817","article-title":"GSDS 2.0: An upgraded gene feature visualization server","volume":"31","author":"Hu","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"D458","DOI":"10.1093\/nar\/gkaa937","article-title":"SMART: Recent updates, new developments and status in 2020","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e4792","DOI":"10.1002\/pro.4792","article-title":"UCSF ChimeraX: Tools for structure building and analysis","volume":"32","author":"Meng","year":"2023","journal-title":"Protein Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1163\/15685403-00003823","article-title":"Identification of suitable reference genes of Scylla paramamosain for gene expression profiling in various tissues and under vibrio challenge","volume":"91","author":"Yabo","year":"2018","journal-title":"Crustaceana"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/23\/12746\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:40:35Z","timestamp":1760114435000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/23\/12746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,27]]},"references-count":56,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["ijms252312746"],"URL":"https:\/\/doi.org\/10.3390\/ijms252312746","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,27]]}}}