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Innovation","award":["317157\/2023-0"],"award-info":[{"award-number":["317157\/2023-0"]}]},{"name":"NMR laboratory of the Multiuser Center for Biomolecular Innovation","award":["310898\/2021-8"],"award-info":[{"award-number":["310898\/2021-8"]}]},{"name":"NMR laboratory of the Multiuser Center for Biomolecular Innovation","award":["AUXEP: 1548\/2020"],"award-info":[{"award-number":["AUXEP: 1548\/2020"]}]},{"name":"NMR laboratory of the Multiuser Center for Biomolecular Innovation","award":["2023\/09642-7"],"award-info":[{"award-number":["2023\/09642-7"]}]},{"name":"NMR laboratory of the Multiuser Center for Biomolecular Innovation","award":["FAPESP 2009\/53989-4"],"award-info":[{"award-number":["FAPESP 2009\/53989-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The respiratory syncytial virus (RSV) matrix protein plays key roles in the virus life cycle and is essential for budding, as it stimulates the optimal membrane curvature necessary for the emergence of viral particles. Resveratrol, a polyphenol (3,4\u2032,5-trihydroxy-trans-stilbene) produced by plants, exhibits pharmacological effects, including anti-inflammatory and antiviral activities. In this study, resveratrol was tested in HEp-2 (Epidermoid carcinoma of the larynx cell) cells for its post-infection effects, and recombinant M protein was produced to characterize the biophysical mechanisms underlying this interaction. The CC50 (Cytotoxic concentration 50%) value for resveratrol was determined to be 297 \u03bcM over 48 h, and the results from the HEp-2 cell cultures demonstrated a viral inhibition of 42.7% in the presence of resveratrol, with an EC50 (Half maximal effective concentration) of 44.26 \u03bcM. This mechanism may occur through interaction with the M protein responsible for the budding of mature viral particles. Biophysical assays enabled us to characterize the interaction of the M\/resveratrol complex as an entropically driven bond, guided by hydrophobic interactions at the dimerization interface of the M protein, which is essential for the stabilization and formation of the oligomers necessary for viral budding. These findings suggest that one of the targets for resveratrol binding is the M protein, indicating a potential site for blocking the progression of the infection.<\/jats:p>","DOI":"10.3390\/ijms252312790","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T03:21:23Z","timestamp":1732764083000},"page":"12790","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Interaction of Human Respiratory Syncytial Virus (HRSV) Matrix Protein with Resveratrol Shows Antiviral Effect"],"prefix":"10.3390","volume":"25","author":[{"given":"Thaina","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Department of Physics, Institute of Biosciences, Humanities and Exact Sciences, S\u00e3o Paulo State University (UNESP), Rua Crist\u00f3v\u00e3o Colombo, 2265, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"},{"name":"Multiuser Center for Biomolecular Innovation (CMIB), S\u00e3o Paulo State University (UNESP), S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6567-3367","authenticated-orcid":false,"given":"Jefferson de Souza","family":"Busso","sequence":"additional","affiliation":[{"name":"Department of Physics, Institute of Biosciences, Humanities and Exact Sciences, S\u00e3o Paulo State University (UNESP), Rua Crist\u00f3v\u00e3o Colombo, 2265, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"},{"name":"Multiuser Center for Biomolecular Innovation (CMIB), S\u00e3o Paulo State University (UNESP), S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1167-9170","authenticated-orcid":false,"given":"Raphael Vinicius Rodrigues","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Physics, Institute of Biosciences, Humanities and Exact 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