{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:32:20Z","timestamp":1785929540693,"version":"3.56.0"},"reference-count":58,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Health","award":["SUBZ.D270.23.017"],"award-info":[{"award-number":["SUBZ.D270.23.017"]}]},{"name":"Polish Ministry of Health","award":["SUBZ.D270.24.072"],"award-info":[{"award-number":["SUBZ.D270.24.072"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Psoriatic arthritis (PsA) and rheumatoid arthritis (RA) are connective tissue autoimmune diseases. The present study aimed to check whether serum clusterin (CLU) concentration and its glycosylation pattern may be markers differentiating these diseases\u2014blood sera of patients with PsA (n = 37), RA (n = 34), and healthy subjects (control, n = 21) were examined. CLU concentration was measured using the ELISA test. Glycosylation was analyzed using lectin-ELISA with sialo-specific lectins from Maackia amurensis (MAA) and Sambucus nigra (SNA) recognizing sialic acid (SA) \u03b12,3- and \u03b12,6-linked, respectively, and fucose-specific lectins from Lotus tetragonolobus (LTA), Ulex europaeus (UEA), and Lens culinaris (LCA) specific to fucose \u03b11,3-linked, \u03b11,2-linked, and core fucose, respectively. Significantly higher CLU concentrations were observed in the PsA than in the RA patients. The expression of \u03b12,6-linked SA was significantly higher in the PsA and RA patients than in the control. The expression of SNA-reactive SA was visibly higher in the PsA compared to the RA and control group but insignificant. Negative significant correlations between CLU concentrations and its glycans reactivity with LTA and UEA were also observed. Significantly higher serum CLU concentration, accompanied by a high expression of SNA-reactive SA and a reduced degree of Lewisx and Lewisy antennary fucosylation, may constitute a promising panel of parameters differentiating PsA from RA.<\/jats:p>","DOI":"10.3390\/ijms252313060","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T04:13:03Z","timestamp":1733371983000},"page":"13060","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Glycosylation Pattern of Serum Clusterin in Psoriatic Arthritis and Rheumatoid Arthritis\u2014The Search for New Diagnostic Glycomarkers"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2948-4574","authenticated-orcid":false,"given":"Ewa Maria","family":"Kratz","sequence":"first","affiliation":[{"name":"Division of Laboratory Diagnostics, Department of Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Borowska Street 211A, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Kacperczyk","sequence":"additional","affiliation":[{"name":"Division of Laboratory Diagnostics, Department of Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Borowska Street 211A, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3711-8614","authenticated-orcid":false,"given":"Izabela","family":"Kokot","sequence":"additional","affiliation":[{"name":"Division of Laboratory Diagnostics, Department of Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Borowska Street 211A, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Piwowar","sequence":"additional","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy, Wroclaw Medical University, Borowska Street 211, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1388-1989","authenticated-orcid":false,"given":"Bogus\u0142awa","family":"Konopska","sequence":"additional","affiliation":[{"name":"Division of Laboratory Diagnostics, Department of Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Borowska Street 211A, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9866-2066","authenticated-orcid":false,"given":"Renata","family":"Sokolik","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Internal Medicine, Wroclaw Medical University, Borowska Street 213, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucyna","family":"Korman","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Internal Medicine, Wroclaw Medical University, Borowska Street 213, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s13075-017-1497-4","article-title":"The epidemiology of psoriatic arthritis in Israel\u2014A population-based study","volume":"20","author":"Eder","year":"2018","journal-title":"Arthritis Res. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1002\/acr.23743","article-title":"Trends in the Prevalence and Incidence of Psoriasis and Psoriatic Arthritis in Ontario, Canada: A Population-Based Study","volume":"71","author":"Eder","year":"2019","journal-title":"Arthritis Care Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1001\/archderm.141.12.1537","article-title":"Prevalence and treatment of psoriasis in the United Kingdom: A population-based study","volume":"141","author":"Gelfand","year":"2005","journal-title":"Arch. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1046\/j.1087-0024.2003.09102.x","article-title":"Psoriasis is common, carries a substantial burden even when not extensive, and is associated with widespread treatment dissatisfaction","volume":"9","author":"Stern","year":"2004","journal-title":"J. Investig. Dermatol. Symp. Proc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1016\/S0140-6736(18)30830-4","article-title":"The pathogenesis of psoriatic arthritis","volume":"391","author":"Veale","year":"2018","journal-title":"Lancet"},{"key":"ref_6","first-page":"52","article-title":"Laboratory findings in psoriatic arthritis","volume":"59","author":"Punzi","year":"2007","journal-title":"Reumatismo"},{"key":"ref_7","first-page":"511","article-title":"Antibodies to cyclic citrullinated peptides in psoriatic arthritis","volume":"32","author":"Bogliolo","year":"2005","journal-title":"J. Rheumatol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Silvy, F., Bertin, D., Bardin, N., Auger, I., Guzian, M.C., Mattei, J.P., Guis, S., Roudier, J., and Balandraud, N. (2015). Antinuclear antibodies in patients with psoriatic arthritis treated or not with biologics. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0134218"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1136\/ard.2004.031286","article-title":"Autoantibodies in biological agent naive patients with psoriatic arthritis","volume":"64","author":"Johnson","year":"2005","journal-title":"Ann. Rheum. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e000656","DOI":"10.1136\/rmdopen-2018-000656","article-title":"Distinguishing rheumatoid arthritis from psoriatic arthritis","volume":"4","author":"Merola","year":"2018","journal-title":"RMD Open"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1136\/annrheumdis-2011-200387","article-title":"Comparison of health-related quality of life in rheumatoid arthritis, psoriatic arthritis and psoriasis and effects of etanercept treatment","volume":"71","author":"Strand","year":"2012","journal-title":"Ann. Rheum. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2665","DOI":"10.1002\/art.21972","article-title":"Classification criteria for psoriatic arthritis: Development of new criteria from a large international study","volume":"54","author":"Taylor","year":"2006","journal-title":"Arthritis Rheum."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1002\/art.27584","article-title":"2010 Rheumatoid arthritis classification criteria: An American College of Rheumatology\/European League Against Rheumatism collaborative initiative","volume":"62","author":"Aletaha","year":"2010","journal-title":"Arthritis Rheum."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"154","DOI":"10.3899\/jrheum.110845","article-title":"The ClASsification for Psoriatic ARthritis (CASPAR) criteria\u2014A retrospective feasibility, sensitivity, and specificity study","volume":"39","author":"Tillett","year":"2012","journal-title":"J. Rheumatol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Geng, Y., Song, Z., Zhang, X., Deng, X., Wang, Y., and Zhang, Z. (2022). Improved diagnostic performance of CASPAR criteria with integration of ultrasound. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.935132"},{"key":"ref_16","first-page":"7209518","article-title":"Onychomycosis in Psoriatic Patients with Nail Disorders: Aetiological Agents and Immunosuppressive Therapy","volume":"2020","author":"Moreira","year":"2020","journal-title":"Dermatol. Res. Pract."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.rdc.2015.07.001","article-title":"The Epidemiology of Psoriatic Arthritis","volume":"41","author":"Ogdie","year":"2015","journal-title":"Rheum. Dis. Clin. N. Am."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1056\/NEJMra1505557","article-title":"Psoriatic Arthritis","volume":"376","author":"Ritchlin","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1093\/rheumatology\/keab865","article-title":"Musculoskeletal pain in psoriasis-relation to inflammation and additional value of ultrasound in psoriatic arthritis classification","volume":"61","author":"Felbo","year":"2022","journal-title":"Rheumatology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1136\/annrheumdis-2013-204858","article-title":"Diagnostic delay of more than 6 months contributes to poor radiographic and functional outcome in psoriatic arthritis","volume":"74","author":"Haroon","year":"2015","journal-title":"Ann. Rheum. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/nature01661","article-title":"Evolving concepts of rheumatoid arthritis","volume":"423","author":"Firestein","year":"2003","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mueller, A.L., Payandeh, Z., Mohammadkhani, N., Mubarak, S.M.H., Zakeri, A., Bahrami, A.A., Brockmueller, A., and Shakibaei, M. (2021). Recent advances in understanding the pathogenesis of rheumatoid arthritis: New treatment strategies. Cells, 10.","DOI":"10.3390\/cells10113017"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s42358-018-0042-8","article-title":"Clinical and pathophysiologic relevance of autoantibodies in rheumatoid arthritis","volume":"59","author":"Baldo","year":"2019","journal-title":"Adv. Rheumatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/1756-185X.12129","article-title":"Anti-citrullinated protein antibodies and their clinical utility in rheumatoid arthritis","volume":"16","author":"Farid","year":"2013","journal-title":"Int. J. Rheum. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1002\/pro.5560061007","article-title":"Identification and characterization of glycosylation sites in human serum clusterin","volume":"6","author":"Kapron","year":"1997","journal-title":"Protein Sci."},{"key":"ref_26","first-page":"152","article-title":"Clusterin and psoriasis, what is the relation?","volume":"2","author":"Hashem","year":"2020","journal-title":"Int. J. Med. Arts"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Holmannova, D., Borsky, P., Borska, L., Andrys, C., Hamakova, K., Rehacek, V., Svadlakova, T., Malkova, A., Beranek, M., and Palicka, V. (2020). Metabolic syndrome, clusterin and elafin in patients with psoriasis vulgaris. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21165617"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Krop\u00e1\u010dkov\u00e1, T., Mann, H., R\u016f\u017ei\u010dkov\u00e1, O., \u0160l\u00e9glov\u00e1, O., Vernerov\u00e1, L., Horv\u00e1thov\u00e1, V., Tom\u010d\u00edk, M., Pavelka, K., Vencovsk\u00fd, J., and \u0160enolt, L. (2021). Clusterin serum levels are elevated in patients with early rheumatoid arthritis and predict disease activity and treatment response. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-90973-2"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/s41392-024-01886-1","article-title":"Glycosylation: Mechanisms, biological functions and clinical implications","volume":"9","author":"He","year":"2024","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3919","DOI":"10.1007\/s00018-011-0684-8","article-title":"Clusterin facilitates in vivo clearance of extracellular misfolded proteins","volume":"68","author":"Wyatt","year":"2011","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7714","DOI":"10.1016\/S0021-9258(18)32238-5","article-title":"Purification and characterization of a cell-aggregating factor (clusterin), the major glycoprotein in ram rete testis fluid","volume":"258","author":"Blaschuk","year":"1983","journal-title":"J. Biol. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7657876","DOI":"10.1155\/2022\/7657876","article-title":"The Influence of Clusterin Glycosylation Variability on Selected Pathophysiological Processes in the Human Body","volume":"2022","author":"Janiszewska","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1158\/1541-7786.MCR-09-0277","article-title":"Clusterin facilitates COMMD1 and I-\u03baB degradation to enhance NF-\u03baB activity in prostate cancer cells","volume":"8","author":"Zoubeidi","year":"2010","journal-title":"Mol. Cancer Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7123","DOI":"10.1073\/pnas.86.18.7123","article-title":"Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: Identity to sulfated glycoprotein 2, a constituent of rat testis fluid","volume":"86","author":"Jenne","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1136\/annrheumdis-2014-205417","article-title":"Discovery and confirmation of a protein biomarker panel with potential to predict response to biological therapy in psoriatic arthritis","volume":"75","author":"Ademowo","year":"2016","journal-title":"Ann. Rheum. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1159\/000366365","article-title":"The chaperone activity of clusterin is dependent on glycosylation and redox environment","volume":"34","author":"Rohne","year":"2014","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1021\/acs.jproteome.5b01069","article-title":"N-Glycoproteomics of Human Seminal Plasma Glycoproteins","volume":"15","author":"Saraswat","year":"2016","journal-title":"J. Proteome Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kokot, I., Mazurek, S., Piwowar, A., Sokolik, R., Rodak, K., Kacperczyk, M., Szostak, R., Cuprych, P., Korman, L., and Maria Kratz, E. (2024). Comparative profiling of serum biomarkers and ATR-FTIR spectroscopy for differential diagnosis of patients with rheumatoid and psoriatic arthritis \u2212 a pilot study. Spectrochim. Acta Part A Mol. Biomol. Spectrosc., 321.","DOI":"10.1016\/j.saa.2024.124654"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Giang, J., Seelen, M.A.J., van Doorn, M.B.A., Rissmann, R., Prens, E.P., and Damman, J. (2018). Complement activation in inflammatory skin diseases. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.00639"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7464","DOI":"10.1021\/bi962895r","article-title":"Potent inhibition of terminal complement assembly by clusterin: Characterization of its impact on C9 polymerization","volume":"36","author":"McDonald","year":"1997","journal-title":"Biochemistry"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e2215","DOI":"10.1038\/cddis.2016.113","article-title":"Clusterin facilitates apoptotic cell clearance and prevents apoptotic cell-induced autoimmune responses","volume":"7","author":"Cunin","year":"2016","journal-title":"Cell Death Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s00281-017-0627-z","article-title":"The role of autoantibodies in the pathophysiology of rheumatoid arthritis","volume":"39","author":"Derksen","year":"2017","journal-title":"Semin. Immunopathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.1016\/S0140-6736(17)31472-1","article-title":"Pathogenetic insights from the treatment of rheumatoid arthritis","volume":"389","author":"McInnes","year":"2017","journal-title":"Lancet"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"473","DOI":"10.4161\/cc.8.3.7652","article-title":"Interleukin-6 affects cell death escaping mechanisms acting on Bax-Ku70-Clusterin interactions in human colon cancer progression","volume":"8","author":"Pucci","year":"2009","journal-title":"Cell Cycle"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.semarthrit.2016.05.012","article-title":"Psoriasis, psoriatic arthritis, and rheumatoid arthritis: Is all inflammation the same?","volume":"46","author":"Coates","year":"2016","journal-title":"Semin. Arthritis Rheum."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"713618","DOI":"10.1155\/2012\/713618","article-title":"Pathological Role of Interleukin-6 in Psoriatic Arthritis","volume":"2012","author":"Ogata","year":"2012","journal-title":"Arthritis"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"So\u0142kiewicz, K., Kokot, I., Kacperczyk, M., Dymicka-Piekarska, V., Dorf, J., and Kratz, E.M. (2024). Serum Clusterin Concentration and Its Glycosylation Changes as Potential New Diagnostic Markers of SARS-CoV-2 Infection and Recovery Process. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25084198"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Janiszewska, E., Kokot, I., Gilowska, I., Faundez, R., and Kratz, E.M. (2021). The possible association of clusterin fucosylation changes with male fertility disorders. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-95288-w"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5299","DOI":"10.4049\/jimmunol.1101889","article-title":"Semen Clusterin Is a Novel DC-SIGN Ligand","volume":"187","author":"Sabatte","year":"2011","journal-title":"J. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/s41420-024-02180-3","article-title":"Biological function of sialic acid and sialylation in human health and disease","volume":"10","author":"Zhu","year":"2024","journal-title":"Cell Death Discov."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Zhou, X., Yang, G., and Guan, F. (2020). Biological Functions and Analytical Strategies of Sialic Acids in Tumor. Cells, 9.","DOI":"10.3390\/cells9020273"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/S0002-9440(10)64921-8","article-title":"Lectin histochemistry of resected adenocarcinoma of the lung: Helix pomatia agglutinin binding is an independent prognostic factor","volume":"160","author":"Laack","year":"2002","journal-title":"Am. J. Pathol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1023\/A:1018508914551","article-title":"Immobilized lotus tetragonolobus agglutinin binds oligosaccharides containing the Le(x) determinant","volume":"14","author":"Yan","year":"1997","journal-title":"Glycoconj. J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1006\/jmbi.2000.4016","article-title":"Structural basis of carbohydrate recognition by lectin II from Ulex europaeus, a protein with a promiscuous carbohydrate-binding site","volume":"301","author":"Loris","year":"2000","journal-title":"J. Mol. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1093\/glycob\/cwp016","article-title":"Comparative analysis of core-fucose-binding lectins from Lens culinaris and Pisum sativum using frontal affinity chromatography","volume":"19","author":"Tateno","year":"2009","journal-title":"Glycobiology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1046\/j.1432-1327.2000.00958.x","article-title":"\u03b1(1,2)-Fucosylation prevents sialyl Lewis x expression and E-selectin- mediated adhesion of fucosyltransferase VII-transfected cells","volume":"267","author":"Zerfaoui","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1155\/2011\/914710","article-title":"Fucose and Sialic Acid Expressions in Human Seminal Fibronectin and \u03b11-Acid Glycoprotein Associated with Leukocytospermia of Infertile Men","volume":"31","author":"Kratz","year":"2011","journal-title":"Dis. Markers"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.autrev.2009.01.013","article-title":"Clinical performance characteristics of a laboratory test. A practical approach in the autoimmune laboratory","volume":"8","author":"Bossuyt","year":"2009","journal-title":"Autoimmun. Rev."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/23\/13060\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:47:10Z","timestamp":1760114830000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/23\/13060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":58,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["ijms252313060"],"URL":"https:\/\/doi.org\/10.3390\/ijms252313060","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]}}}