{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T13:24:14Z","timestamp":1780320254814,"version":"3.54.1"},"reference-count":40,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T00:00:00Z","timestamp":1733702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 110-2320-B-002-039-MY3"],"award-info":[{"award-number":["MOST 110-2320-B-002-039-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NTU-JP-113L7229"],"award-info":[{"award-number":["NTU-JP-113L7229"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NTU-JP-114L7201"],"award-info":[{"award-number":["NTU-JP-114L7201"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Education, Higher Education SPROUT Project","award":["MOST 110-2320-B-002-039-MY3"],"award-info":[{"award-number":["MOST 110-2320-B-002-039-MY3"]}]},{"name":"Ministry of Education, Higher Education SPROUT Project","award":["NTU-JP-113L7229"],"award-info":[{"award-number":["NTU-JP-113L7229"]}]},{"name":"Ministry of Education, Higher Education SPROUT Project","award":["NTU-JP-114L7201"],"award-info":[{"award-number":["NTU-JP-114L7201"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Weight regain within one year after weight loss is frequently observed and is referred to as yo-yo dieting or weight cycling. In this study, we explore the effects of yo-yo dieting on the liver, adipose tissue, and muscle characteristics of male zebrafish. Four-month-old AB wild-type male zebrafish were randomly assigned to three groups: high-calorie intake (H, seven meals per day), low-calorie intake (L, two meals per day), and yo-yo diet (the low- and high-calorie alternation switched every two weeks) groups. Feeding the fish the H diet for over 8 weeks led to steatosis and damage to the liver. The yo-yo diet reduced liver lipid accumulation at week eight but caused a similar degree of lipid accumulation as the H diet thereafter. It was found that twenty weeks of yo-yo dieting actually exacerbated hepatic damage. Compared to the L diet, feeding the fish on the yo-yo and H diets for a period of 20 weeks significantly increased the size of muscle fibers, resulting in higher speed during burst swimming and a significant increase in the size and number of adipocytes in the abdominal tissue. To summarize, short-term yo-yo dieting was found to attenuate hepatosteatosis and maintain fast-twitch muscle function. Long-term yo-yo dieting preserved fast-twitch muscle function and muscle fiber size; however, it exacerbated the pathological changes in the liver.<\/jats:p>","DOI":"10.3390\/ijms252313225","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T11:16:48Z","timestamp":1733743008000},"page":"13225","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long-Term Yo-Yo Dieting Exaggerates Liver Steatosis and Lesions but Preserves Muscle Performance in Male Zebrafish"],"prefix":"10.3390","volume":"25","author":[{"given":"Tzu-Chieh","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Hsien","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4524-3263","authenticated-orcid":false,"given":"I-Hsuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5776-084X","authenticated-orcid":false,"given":"Ching-Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.7326\/0003-4819-122-7-199504010-00001","article-title":"Weight-gain as a risk factor for clinical diabetes-mellitus in women","volume":"122","author":"Colditz","year":"1995","journal-title":"Ann. 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