{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:30:50Z","timestamp":1788859850900,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre, Poland","award":["UMO-2019\/35\/N\/NZ3\/00262"],"award-info":[{"award-number":["UMO-2019\/35\/N\/NZ3\/00262"]}]},{"name":"Medical University of Gdansk","award":["UMO-2019\/35\/N\/NZ3\/00262"],"award-info":[{"award-number":["UMO-2019\/35\/N\/NZ3\/00262"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Antigen presentation plays a critical role in the pathogenesis of immune-mediated disorders. This study aimed to investigate the effects of IFN-\u03b3 and a cytokine mix (5MIX: IL-1\u03b1, IL-17A, IL-22, OsM, and TNF-\u03b1) on the antigen-presenting capabilities of keratinocytes, with a specific focus on immune-mediated dermatological conditions such as psoriasis (Ps). To achieve this, keratinocytes were treated with IFN-\u03b3 and 5MIX, and their impact on the expression of key antigen-presentation molecules, HLA-DR\u03b1 and CD74, was assessed. Transcriptomic analysis revealed that IFN-\u03b3 alone altered the expression of 254 genes, highlighting its central role in modulating immune responses, including the recruitment of immune cells and regulation of inflammation. Temporal experiments further demonstrated that IFN-\u03b3 and 5MIX enhanced early endocytic activity and lysosomal degradation pathways, both essential for effective antigen presentation and T-cell activation. To extend these findings to a clinical context, a co-culture model using keratinocytes derived from psoriatic patients was established. This model revealed increased cytokine production following antigen stimulation, indicating robust and consistent CD4+ and na\u00efve T-cell responses. These results elucidate the complex dynamics of cytokine signaling and antigen presentation in keratinocytes, providing insights into potential therapeutic strategies for immune-mediated skin disorders like Ps.<\/jats:p>","DOI":"10.3390\/ijms252413387","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T05:55:45Z","timestamp":1734069345000},"page":"13387","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Keratinocyte-Mediated Antigen Presentation in Psoriasis: Preliminary Insights from In Vitro Studies"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4612-555X","authenticated-orcid":false,"given":"Katarzyna","family":"Zima","sequence":"first","affiliation":[{"name":"Department of Medical Biology and Genetics, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"},{"name":"Department of Physiology, Medical University of Gdansk, Debinki 1, 80-211 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6089-8030","authenticated-orcid":false,"given":"Dorota","family":"Purzycka-Bohdan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, University Clinical Centre, Mariana Smoluchowskiego 17, 80-214 Gdansk, Poland"},{"name":"Clinical Physiology Unit, Medical Simulation Centre, Medical University of Gdansk, Debowa 25, 80-204 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4040-0116","authenticated-orcid":false,"given":"Aneta","family":"Szczerkowska-Dobosz","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology, Medical University of Gdansk, University Clinical Centre, Mariana Smoluchowskiego 17, 80-214 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2664-8724","authenticated-orcid":false,"given":"Magdalena","family":"Gabig-Cimi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Genetics, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5095293","DOI":"10.1155\/2017\/5095293","article-title":"Skin Immune Landscape: Inside and Outside the Organism","volume":"2017","author":"Abdallah","year":"2017","journal-title":"Mediat. 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