{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T06:11:44Z","timestamp":1777961504533,"version":"3.51.4"},"reference-count":50,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Neutrophils and neutrophil extracellular traps (NETs) contribute to thrombosis and hyperinflammation in myeloproliferative neoplasms (MPN). High-density neutrophils (HDNs) and low-density neutrophils (LDNs) have recently been characterized as distinct neutrophil sub-populations with distinct morphological and functional properties. We aim to study the kinetics of NET formation and inhibition with interferon-\u03b1 (IFN\u03b1) in neutrophils derived from patients with MPN as compared to matched healthy controls. Ex vivo NET formation was assessed by neutrophil-elastase activity, neutrophil-associated nucleosomes, myeloperoxidase (MPO), and citrullinated histone H3 content. IFN\u03b1 significantly inhibited NET formation in neutrophils derived from MPN patients. Neutrophil sub-population analysis demonstrated that HDNs drive the increase in NET formation as compared to LDNs in patients and in healthy controls and are effectively inhibited by IFN\u03b1, an effect that is lost in LDNs. In conclusion, we demonstrate that in MPN, HDNs drive excess NET formation and are more sensitive to IFN\u03b1 inhibition. These observations uncover unique neutrophil sub-population biology and dynamics in MPN.<\/jats:p>","DOI":"10.3390\/ijms252413473","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T09:17:58Z","timestamp":1734340678000},"page":"13473","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Interferon-\u03b1 Inhibits NET Formation in Neutrophils Derived from Patients with Myeloproliferative Neoplasms in a Neutrophil Sub-Population-Specific Manner"],"prefix":"10.3390","volume":"25","author":[{"given":"Shirly","family":"Partouche","sequence":"first","affiliation":[{"name":"Felsenstein Medical Research Center, Beilinson Hospital, Rabin Medical Center, Petah Tikva 4941492, Israel"},{"name":"Institute of Hematology, Davidoff Cancer Center, Beilinson Hospital, Rabin Medical Center, Petah Tikva 4941492, 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Neutrophil","volume":"54","author":"Burn","year":"2021","journal-title":"Immunity"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Langiu, M., Palacios-Acedo, A.L., Crescence, L., Mege, D., Dubois, C., and Panicot-Dubois, L. (2022). Neutrophils, Cancer and Thrombosis: The New Bermuda Triangle in Cancer Research. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031257"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.1111\/febs.14524","article-title":"Cancer-related circulating and tumor-associated neutrophils\u2014Subtypes, sources and function","volume":"285","author":"Shaul","year":"2018","journal-title":"FEBS J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.celrep.2014.12.039","article-title":"Phenotypic diversity and plasticity in circulating neutrophil subpopulations in cancer","volume":"10","author":"Sagiv","year":"2015","journal-title":"Cell Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s12943-023-01843-6","article-title":"The evolution and heterogeneity of neutrophils in cancers: Origins, subsets, functions, orchestrations and clinical applications","volume":"22","author":"Liu","year":"2023","journal-title":"Mol. Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1016\/j.cjca.2024.03.018","article-title":"Low-Density Neutrophils and Neutrophil Extracellular Traps (NETs) Are New Inflammatory Players in Heart Failure","volume":"40","author":"Dumont","year":"2024","journal-title":"Can. J. Cardiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s00281-013-0375-7","article-title":"Low-density granulocytes: A distinct class of neutrophils in systemic autoimmunity","volume":"35","author":"Kaplan","year":"2013","journal-title":"Semin. Immunopathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1080\/14397595.2021.1918883","article-title":"Two distinct subsets of LDGs (low density granulocytes) in ANCA-associated vasculitis","volume":"32","author":"Endo","year":"2021","journal-title":"Mod. Rheumatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.4049\/jimmunol.1801512","article-title":"Genomic Identification of Low-Density Granulocytes and Analysis of Their Role in the Pathogenesis of Systemic Lupus Erythematosus","volume":"202","author":"Kegerreis","year":"2019","journal-title":"J. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.18632\/oncotarget.28135","article-title":"Circulating low density neutrophils of breast cancer patients are associated with their worse prognosis due to the impairment of T cell responses","volume":"12","author":"Saraiva","year":"2021","journal-title":"Oncotarget"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1182\/blood.2022017416","article-title":"Biology and therapeutic targeting of molecular mechanisms in MPNs","volume":"141","author":"How","year":"2023","journal-title":"Blood"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Markovi\u0107, D., Maslovari\u0107, I., Djiki\u0107, D., and \u010coki\u0107, V.P. (2022). Neutrophil Death in Myeloproliferative Neoplasms: Shedding More Light on Neutrophils as a Pathogenic Link to Chronic Inflammation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031490"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1002\/ajh.27079","article-title":"A globally applicable \u201ctriple A\u201d risk model for essential thrombocythemia based on Age, Absolute neutrophil count, and Absolute lymphocyte count","volume":"98","author":"Tefferi","year":"2023","journal-title":"Am. J. Hematol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/s41408-022-00625-5","article-title":"Neutrophil-to-lymphocyte ratio is a novel predictor of venous thrombosis in polycythemia vera","volume":"12","author":"Carobbio","year":"2022","journal-title":"Blood Cancer J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e35","DOI":"10.1002\/ajh.26423","article-title":"Deciphering the individual contribution of absolute neutrophil and monocyte counts to thrombosis risk in polycythemia vera and essential thrombocythemia","volume":"97","author":"Farrukh","year":"2022","journal-title":"Am. J. Hematol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wolach, O., Sellar, R.S., Martinod, K., Cherpokova, D., McConkey, M., Chappell, R.J., Silver, A.J., Adams, D., Castellano, C.A., and Schneider, R.K. (2018). Increased neutrophil extracellular trap formation promotes thrombosis in myeloproliferative neoplasms. Sci. Transl. Med., 10.","DOI":"10.1126\/scitranslmed.aan8292"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3515","DOI":"10.1182\/bloodadvances.2020004061","article-title":"Increased levels of NETosis in Myeloproliferative Neoplasms are not linked to thrombotic events","volume":"5","author":"Schmidt","year":"2021","journal-title":"Blood Adv."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1038\/s41375-019-0500-2","article-title":"High circulating levels of MPO-DNA are associated with thrombosis in patients with MPN","volume":"33","author":"Guy","year":"2019","journal-title":"Leukemia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1097\/MOH.0000000000000690","article-title":"Casting a NET on cancer: The multiple roles for neutrophil extracellular traps in cancer","volume":"29","author":"Wolach","year":"2022","journal-title":"Curr. Opin. Hematol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"20406207241229588","DOI":"10.1177\/20406207241229588","article-title":"Interferons in the treatment of myeloproliferative neoplasms","volume":"15","author":"Vachhani","year":"2024","journal-title":"Ther. Adv. Hematol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1111\/bjh.18845","article-title":"Interferon alpha-2 treatment reduces circulating neutrophil extracellular trap levels in myeloproliferative neoplasms","volume":"202","author":"Massarenti","year":"2023","journal-title":"Br. J. Haematol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.yexcr.2016.03.007","article-title":"Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry","volume":"342","author":"Lakschevitz","year":"2016","journal-title":"Exp. Cell Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Blanco-Camarillo, C., Alem\u00e1n, O.R., and Rosales, C. (2021). Low-Density Neutrophils in Healthy Individuals Display a Mature Primed Phenotype. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.672520"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"305","DOI":"10.3390\/hemato2020018","article-title":"Can Novel Insights into the Pathogenesis of Myeloproliferative Neoplasm-Related Thrombosis Inform Novel Treatment Approaches?","volume":"2","author":"Wolach","year":"2021","journal-title":"Hemato"},{"key":"ref_26","first-page":"205","article-title":"Neutrophils prothrombotic characteristics during myeloproliferative neoplasms","volume":"12","author":"Wolff","year":"2020","journal-title":"Arch. Cardiovasc. Dis. Suppl."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Marin Oyarz\u00fan, C.P., Carestia, A., Lev, P.R., Glembotsky, A.C., Castro R\u00edos, M.A., Moiraghi, B., Molinas, F.C., Marta, R.F., Schattner, M., and Heller, P.G. (2016). Neutrophil extracellular trap formation and circulating nucleosomes in patients with chronic myeloproliferative neoplasms. Sci. Rep., 6.","DOI":"10.1038\/srep38738"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.jtha.2023.08.028","article-title":"Platelets and neutrophils cooperate to induce increased neutrophil extracellular trap formation in JAK2V617F myeloproliferative neoplasms","volume":"22","author":"Guy","year":"2024","journal-title":"J. Thromb. Haemost."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mar\u00edn Oyarz\u00fan, C.P., Glembotsky, A.C., Goette, N.P., Lev, P.R., De Luca, G., Baroni Pietto, M.C., Moiraghi, B., Castro R\u00edos, M.A., Vicente, A., and Marta, R.F. (2020). Platelet Toll-Like Receptors Mediate Thromboinflammatory Responses in Patients with Essential Thrombocythemia. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.00705"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1182\/blood-2023-174584","article-title":"Interaction Between JAK2-Mutated Neutrophils and Platelets Initiates Thrombosis via Neutrophil Extracellular Traps","volume":"142","author":"Ueda","year":"2023","journal-title":"Blood"},{"key":"ref_31","first-page":"70","article-title":"Vascular endothelial cell expression of JAK2V617F is sufficient to promote a pro-thrombotic state due to increased P-selectin expression","volume":"104","author":"Alexandre","year":"2018","journal-title":"Haematologica"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"132184","DOI":"10.1016\/j.ijcard.2024.132184","article-title":"The clonal hematopoiesis mutation JAK2(V617F) aggravates endothelial injury and thrombosis in arteries with erosion-like intimas","volume":"409","author":"Molinaro","year":"2024","journal-title":"Int. J. Cardiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1172\/JCI90312","article-title":"JAK2-V617F promotes venous thrombosis through \u03b21\/\u03b22 integrin activation","volume":"128","author":"Edelmann","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/imr.13161","article-title":"Low-density granulocytes in systemic autoimmunity and autoinflammation","volume":"314","author":"Kaplan","year":"2023","journal-title":"Immunol. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"538","DOI":"10.4049\/jimmunol.1100450","article-title":"Netting neutrophils induce endothelial damage, infiltrate tissues, and expose immunostimulatory molecules in systemic lupus erythematosus","volume":"187","author":"Villanueva","year":"2011","journal-title":"J. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Henning, S., Reimers, T., Abdulahad, W., Fierro, J.J., Doornbos-van der Meer, B., Bootsma, H., Horvath, B., de Leeuw, K., and Westra, J. (2024). Low density granulocytes and neutrophil extracellular trap formation are increased in incomplete systemic lupus erythematosus. Rheumatology.","DOI":"10.1093\/rheumatology\/keae300"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Dumont, B.L., Neagoe, P.E., Charles, E., Villeneuve, L., Tardif, J.C., R\u00e4kel, A., White, M., and Sirois, M.G. (2024). Low-Density Neutrophils Contribute to Subclinical Inflammation in Patients with Type 2 Diabetes. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25031674"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Park, J., Dean, L.S., Heckl, J., Gangcuangco, L.M., Pedro, T.K., Tallquist, M.D., Seto, T.B., Shiramizu, B., Chow, D.C., and Shikuma, C.M. (2023). Low-density granulocytes display immature cells with enhanced NET formation in people living with HIV. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-40475-0"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Dean, L.S., Devendra, G., Jiyarom, B., Subia, N., Tallquist, M.D., Nerurkar, V.R., Chang, S.P., Chow, D.C., Shikuma, C.M., and Park, J. (2022). Phenotypic alteration of low-density granulocytes in people with pulmonary post-acute sequalae of SARS-CoV-2 infection. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1076724"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Domerecka, W., Homa-Mlak, I., Mlak, R., Michalak, A., Wili\u0144ska, A., Kowalska-K\u0119pczy\u0144ska, A., Dreher, P., Cicho\u017c-Lach, H., and Ma\u0142ecka-Massalska, T. (2022). Indicator of Inflammation and NETosis-Low-Density Granulocytes as a Biomarker of Autoimmune Hepatitis. J. Clin. Med., 11.","DOI":"10.3390\/jcm11082174"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.3324\/haematol.2012.082560","article-title":"Increased reactive oxygen species production and p47phox phosphorylation in neutrophils from myeloproliferative disorders patients with JAK2 (V617F) mutation","volume":"98","author":"Margarita","year":"2013","journal-title":"Haematologica"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1182\/blood-2013-03-460154","article-title":"Myeloproliferative neoplasms and thrombosis","volume":"122","author":"Barbui","year":"2013","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"100126","DOI":"10.1016\/j.rpth.2023.100126","article-title":"Neutrophils in lung cancer patients: Activation potential and neutrophil extracellular trap formation","volume":"7","author":"Mauracher","year":"2023","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1093\/rheumatology\/keaa532","article-title":"Neutrophil subpopulations and their activation potential in patients with antiphospholipid syndrome and healthy individuals","volume":"60","author":"Mauracher","year":"2021","journal-title":"Rheumatology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jhep.2022.08.029","article-title":"Neutrophil extracellular traps contribute to liver damage and increase defective low-density neutrophils in alcohol-associated hepatitis","volume":"78","author":"Cho","year":"2023","journal-title":"J. Hepatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e516","DOI":"10.1097\/HS9.0000000000000516","article-title":"Challenges and Perspectives for Therapeutic Targeting of Myeloproliferative Neoplasms","volume":"5","author":"Brkic","year":"2021","journal-title":"Hemasphere"},{"key":"ref_47","first-page":"1724","article-title":"Neutrophil extracellular traps: How to generate and visualize them","volume":"36","author":"Brinkmann","year":"2010","journal-title":"J. Vis. Exp."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"102497","DOI":"10.1016\/j.xpro.2023.102497","article-title":"Protocol for density gradient neutrophil isolation and flow cytometry-based characterization from human peripheral blood","volume":"4","author":"Kuang","year":"2023","journal-title":"STAR Protoc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1016\/j.celrep.2019.11.021","article-title":"Visualizing the Selectivity and Dynamics of Interferon Signaling In Vivo","volume":"29","author":"Stifter","year":"2019","journal-title":"Cell Rep."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Goldberg, I., Partouche, S., Halperin, E., Atamna, B., Shacham Abulafia, A., Shapira, S., Samara, A., Gover-Proaktor, A., Leader, A., and Spectre, G. (2024, January 7\u201310). Interferon Inhibits Netosis in Neutrophils Derived from Patients with Myeloproliferative Neoplasms in a Neutrophil Subpopulation-Specific Manner. Proceedings of the Annual Meeting of the American Society of Hematology, San Diego, CA, USA.","DOI":"10.1182\/blood-2024-209795"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/24\/13473\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:53:10Z","timestamp":1760115190000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/24\/13473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,16]]},"references-count":50,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["ijms252413473"],"URL":"https:\/\/doi.org\/10.3390\/ijms252413473","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,16]]}}}