{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:17:21Z","timestamp":1762255041211,"version":"build-2065373602"},"reference-count":74,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Key Project of Yunnan Province","award":["202202AA100001","82171817","202002AA100009"],"award-info":[{"award-number":["202202AA100001","82171817","202002AA100009"]}]},{"name":"National Natural Science Foundation of China","award":["202202AA100001","82171817","202002AA100009"],"award-info":[{"award-number":["202202AA100001","82171817","202002AA100009"]}]},{"name":"Major Science and Technology Special Projects of Yunnan Province","award":["202202AA100001","82171817","202002AA100009"],"award-info":[{"award-number":["202202AA100001","82171817","202002AA100009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Due to the complex pathogenic and immune escape mechanisms of herpes simplex virus type 1 (HSV-1), especially the failure of induced immune responses to block the initial cell-to-cell transmission of the virus from skin cells to neurons, the body struggles to establish effective prevention and control methods, resulting in the failure of currently developed vaccines. Previous studies have highlighted the crucial roles of surface glycoproteins and nucleocapsid proteins in activating the body\u2019s immune defense system against HSV-1 infection. In this study, recombinant adenoviruses were used as vectors to generate adenoviruses carrying the nucleocapsid protein genes UL18 and UL25, as well as the surface glycoprotein gene gB. This approach aimed to mimic the protein expression process that occurs following viral infection of the host and to investigate the immune response characteristics induced by UL18, UL25, and gB proteins. The findings revealed that UL18, UL25, and gB proteins could all trigger the expression of genes associated with innate immune responses; however, the specific genes induced varied in type and level. Furthermore, all three proteins were capable of promoting the proliferation of CD8+ T cells in the lymph nodes. Notably, only UL18 and gB could elicit a Th1 cell immune response. Interestingly, among these proteins, only UL18 could also induce a relatively higher IL-4 level, indicating a Th2 cell immune response. In addition to cellular immunity, all three proteins stimulated the production of specific IgG antibodies. Notably, UL18 induced higher and more sustained levels of specific IgG antibodies in mice. By contrast, only glycoprotein gB induced lower levels of neutralizing antibodies in mice. Moreover, when these mice were challenged with HSV-1, the co-immunization with UL18 and gB provided better protection than gB alone. In conclusion, HSV-1 surface glycoproteins and nucleocapsid proteins exhibit differences in their ability to induce innate and adaptive immunity in the body, suggesting potential avenues for vaccine design by leveraging their complementary advantages.<\/jats:p>","DOI":"10.3390\/ijms252413486","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T12:09:28Z","timestamp":1734350968000},"page":"13486","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immune Response Elicited by Recombinant Adenovirus-Delivered Glycoprotein B and Nucleocapsid Protein UL18 and UL25 of HSV-1 in Mice"],"prefix":"10.3390","volume":"25","author":[{"given":"Haobo","family":"Zhang","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"School of Life Sciences, Yunnan University, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming 650118, China"},{"name":"School of Life Sciences, Yunnan University, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Liao","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming 650118, 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2670","DOI":"10.1080\/21505594.2021.1982373","article-title":"Pathogenesis and virulence of herpes simplex virus","volume":"12","author":"Zhu","year":"2021","journal-title":"Virulence"},{"key":"ref_2","unstructured":"(2023, April 05). WHO. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/herpes-simplex-virus."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e300","DOI":"10.1016\/S2214-109X(16)30362-X","article-title":"First estimates of the global and regional incidence of neonatal herpes infection","volume":"5","author":"Looker","year":"2017","journal-title":"Lancet Glob. Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/s41579-020-00448-w","article-title":"The structural basis of herpesvirus entry","volume":"19","author":"Connolly","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.tim.2012.08.005","article-title":"A cultured affair: HSV latency and reactivation in neurons","volume":"20","author":"Wilson","year":"2012","journal-title":"Trends Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Verzosa, A.L., Mcgeever, L.A., Bhark, S.J., Delgado, T., Salazar, N., and Sanchez, E.L. (2021). Herpes Simplex Virus 1 Infection of Neuronal and Non-Neuronal Cells Elicits Specific Innate Immune Responses and Immune Evasion Mechanisms. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.644664"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1007\/s13365-011-0063-0","article-title":"Control of HSV-1 latency in human trigeminal ganglia--current overview","volume":"17","author":"Held","year":"2011","journal-title":"J. Neurovirol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1016\/S0140-6736(00)04638-9","article-title":"Herpes simplex virus infections","volume":"357","author":"Whitley","year":"2001","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.tim.2020.03.003","article-title":"Herpes Simplex Virus-1 in the Brain: The Dark Side of a Sneaky Infection","volume":"28","author":"Marcocci","year":"2020","journal-title":"Trends Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"eadf4904","DOI":"10.1126\/sciadv.adf4904","article-title":"Stacking the odds: Multiple sites for HSV-1 latency","volume":"9","author":"Wang","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1086\/424519","article-title":"Valacyclovir and acyclovir for suppression of shedding of herpes simplex virus in the genital tract","volume":"190","author":"Gupta","year":"2004","journal-title":"J. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"122","DOI":"10.2340\/00015555-0806","article-title":"Prevention of ulcerative lesions by episodic treatment of recurrent herpes labialis: A literature review","volume":"90","author":"Harmenberg","year":"2010","journal-title":"Acta Derm. Venereol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1128\/AAC.02250-12","article-title":"In vivo anti-herpes simplex virus activity of a sulfated derivative of Agaricus brasiliensis mycelial polysaccharide","volume":"57","author":"Cardozo","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_14","first-page":"137","article-title":"Contemporary approaches to vaccination against herpes simplex virus","volume":"179","author":"Burke","year":"1992","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1016\/S0264-410X(98)00434-4","article-title":"Herpes simplex virus vaccines","volume":"17","author":"Bernstein","year":"1999","journal-title":"Vaccine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1006\/viro.1999.9653","article-title":"Immunogenicity of herpes simplex virus type 1 mutants containing deletions in one or more alpha-genes: ICP4, ICP27, ICP22, and ICP0","volume":"256","author":"Brehm","year":"1999","journal-title":"Virology"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Krishnan, R., and Stuart, P.M. (2021). Developments in Vaccination for Herpes Simplex Virus. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.798927"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ralph, M., Bednarchik, M., Tomer, E., Rafael, D., Zargarian, S., Gerlic, M., and Kobiler, O. (2017). Promoting Simultaneous Onset of Viral Gene Expression Among Cells Infected with Herpes Simplex Virus-1. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02152"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6845","DOI":"10.1016\/j.vaccine.2009.09.017","article-title":"Immunization with HSV-1 glycoprotein C prevents immune evasion from complement and enhances the efficacy of an HSV-1 glycoprotein D subunit vaccine","volume":"27","author":"Awasthi","year":"2009","journal-title":"Vaccine"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1056\/NEJMoa1103151","article-title":"Efficacy results of a trial of a herpes simplex vaccine","volume":"366","author":"Belshe","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1016\/j.vaccine.2010.12.031","article-title":"An ocular mucosal administration of nanoparticles containing DNA vaccine pRSC-gD-IL-21 confers protection against mucosal challenge with herpes simplex virus type 1 in mice","volume":"29","author":"Hu","year":"2011","journal-title":"Vaccine"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Egan, K.P., Awasthi, S., Tebaldi, G., Hook, L.M., Naughton, A.M., Fowler, B.T., Beattie, M., Alameh, M.G., Weissman, D., and Cohen, G.H. (2023). A Trivalent HSV-2 gC2, gD2, gE2 Nucleoside-Modified mRNA-LNP Vaccine Provides Outstanding Protection in Mice against Genital and Non-Genital HSV-1 Infection, Comparable to the Same Antigens Derived from HSV-1. Viruses, 15.","DOI":"10.3390\/v15071483"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Keil, T., Liu, D., Lloyd, M., Coombs, W., Moffat, J., and Visalli, R. (2020). DNA Encapsidation and Capsid Assembly Are Underexploited Antiviral Targets for the Treatment of Herpesviruses. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01862"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Li, X., Wang, J., Mou, T., Gao, Y., Wang, L., Fan, S., Xu, X., Jiang, G., Cui, P., and Xu, X. (2021). Immunological Identification and Characterization of the Capsid Scaffold Protein Encoded by UL26.5 of Herpes Simplex Virus Type 2. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.649722"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s00109-010-0696-0","article-title":"Development of adenoviral vector-based mucosal vaccine against influenza","volume":"89","author":"Tutykhina","year":"2011","journal-title":"J. Mol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/s41392-023-01408-5","article-title":"Viral vectored vaccines: Design, development, preventive and therapeutic applications in human diseases","volume":"8","author":"Wang","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"812","DOI":"10.3858\/emm.2009.41.11.087","article-title":"Modulation of protective immunity against herpes simplex virus via mucosal genetic co-transfer of DNA vaccine with beta2-adrenergic agonist","volume":"41","author":"Kim","year":"2009","journal-title":"Exp. Mol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e02156-17","DOI":"10.1128\/JVI.02156-17","article-title":"Laser Adjuvant-Assisted Peptide Vaccine Promotes Skin Mobilization of Dendritic Cells and Enhances Protective CD8+ TEM and TRM Cell Responses against Herpesvirus Infection and Disease","volume":"92","author":"Lopes","year":"2018","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1038\/sj.embor.7400807","article-title":"Molecular gymnastics at the herpesvirus surface","volume":"7","author":"Rey","year":"2006","journal-title":"EMBO Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1038\/sj.gt.3302393","article-title":"Cytokine genetic adjuvant facilitates prophylactic intravascular DNA vaccine against acute and latent herpes simplex virus infection in mice","volume":"12","author":"Cui","year":"2005","journal-title":"Gene Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vaccine.2015.10.137","article-title":"A novel HSV-2 subunit vaccine induces GLA-dependent CD4 and CD8 T cell responses and protective immunity in mice and guinea pigs","volume":"34","author":"Odegard","year":"2016","journal-title":"Vaccine"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"12698","DOI":"10.1128\/JVI.05791-11","article-title":"A domain in the herpes simplex virus 1 triplex protein VP23 is essential for closure of capsid shells into icosahedral structures","volume":"85","author":"Kim","year":"2011","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1146\/annurev.micro.51.1.311","article-title":"Interaction of antigens and antibodies at mucosal surfaces","volume":"51","author":"Lamm","year":"1997","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"6692","DOI":"10.1128\/JVI.77.12.6692-6699.2003","article-title":"Protection against recurrent ocular herpes simplex virus type 1 disease after therapeutic vaccination of latently infected mice","volume":"77","author":"Richards","year":"2003","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Xu, X., Feng, X., Wang, L., Yi, T., Zheng, L., Jiang, G., Fan, S., Liao, Y., Feng, M., and Zhang, Y. (2020). A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008703"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"186","DOI":"10.4049\/jimmunol.1700145","article-title":"Bolstering the Number and Function of HSV-1-Specific CD8+ Effector Memory T Cells and Tissue-Resident Memory T Cells in Latently Infected Trigeminal Ganglia Reduces Recurrent Ocular Herpes Infection and Disease","volume":"199","author":"Khan","year":"2017","journal-title":"J. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1186\/1743-422X-8-481","article-title":"Herpes simplex virus infects most cell types in vitro: Clues to its success","volume":"8","author":"Karasneh","year":"2011","journal-title":"Virol. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12292","DOI":"10.1128\/JVI.01700-10","article-title":"Cascade of events governing cell-cell fusion induced by herpes simplex virus glycoproteins gD, gH\/gL, and gB","volume":"84","author":"Atanasiu","year":"2010","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.coviro.2016.01.010","article-title":"gH\/gL supercomplexes at early stages of herpesvirus entry","volume":"18","author":"Heldwein","year":"2016","journal-title":"Curr. Opin. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.virol.2010.01.032","article-title":"Human antibodies to herpes simplex virus type 1 glycoprotein C are neutralizing and target the heparan sulfate-binding domain","volume":"400","author":"Adamiak","year":"2010","journal-title":"Virology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"105298","DOI":"10.1016\/j.antiviral.2022.105298","article-title":"A potent neutralizing and protective antibody against a conserved continuous epitope on HSV glycoprotein D","volume":"201","author":"Tian","year":"2022","journal-title":"Antivir. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0161-5890(98)00051-0","article-title":"A dominant V beta bias in the CTL response after HSV-1 infection is determined by peptide residues predicted to also interact with the TCR beta-chain CDR3","volume":"35","author":"Turner","year":"1998","journal-title":"Mol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"702","DOI":"10.4049\/jimmunol.145.2.702","article-title":"Herpes simplex virus glycoprotein D is recognized as antigen by CD4+ and CD8+ T lymphocytes from infected mice. Characterization of T cell clones","volume":"145","author":"Johnson","year":"1990","journal-title":"J. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1080\/14760584.2021.1960162","article-title":"Current vaccine approaches and emerging strategies against herpes simplex virus (HSV)","volume":"20","author":"Wijesinghe","year":"2021","journal-title":"Expert Rev. Vaccines"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e161968","DOI":"10.1172\/JCI161968","article-title":"A non-neutralizing glycoprotein B monoclonal antibody protects against herpes simplex virus disease in mice","volume":"133","author":"Kuraoka","year":"2023","journal-title":"J. Clin. Investig."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Fukui, A., Maruzuru, Y., Ohno, S., Nobe, M., Iwata, S., Takeshima, K., Koyanagi, N., Kato, A., Kitazume, S., and Yamaguchi, Y. (2023). Dual impacts of a glycan shield on the envelope glycoprotein B of HSV-1: Evasion from human antibodies in vivo and neurovirulence. mBio, 14.","DOI":"10.1128\/mbio.00992-23"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.tim.2011.09.001","article-title":"Herpes simplex virus capsid assembly and DNA packaging: A present and future antiviral drug target","volume":"19","author":"Baines","year":"2011","journal-title":"Trends Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Brandariz-Nu\u00f1ez, A., Robinson, S.J., and Evilevitch, A. (2020). Pressurized DNA state inside herpes capsids-A novel antiviral target. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008604"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1007\/s10875-010-9441-2","article-title":"Diversity in CD8+ T cell function and epitope breadth among persons with genital herpes","volume":"30","author":"Laing","year":"2010","journal-title":"J. Clin. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"12899","DOI":"10.1073\/pnas.2131705100","article-title":"Immunodominance among herpes simplex virus-specific CD8 T cells expressing a tissue-specific homing receptor","volume":"100","author":"Koelle","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.coviro.2013.04.004","article-title":"T-cell immunity to human alphaherpesviruses","volume":"3","author":"Ouwendijk","year":"2013","journal-title":"Curr. Opin. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1172\/JCI60556","article-title":"Cross-presentation and genome-wide screening reveal candidate T cells antigens for a herpes simplex virus type 1 vaccine","volume":"122","author":"Jing","year":"2012","journal-title":"J. Clin. Investig."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11422","DOI":"10.1128\/JVI.74.23.11422-11425.2000","article-title":"CD4 T-cell responses to herpes simplex virus type 2 major capsid protein VP5: Comparison with responses to tegument and envelope glycoproteins","volume":"74","author":"Koelle","year":"2000","journal-title":"J. Virol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8497","DOI":"10.1128\/JVI.01089-15","article-title":"A Dual-Modality Herpes Simplex Virus 2 Vaccine for Preventing Genital Herpes by Using Glycoprotein C and D Subunit Antigens to Induce Potent Antibody Responses and Adenovirus Vectors Containing Capsid and Tegument Proteins as T Cell Immunogens","volume":"89","author":"Awasthi","year":"2015","journal-title":"J. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1080\/21645515.2017.1323604","article-title":"A trivalent subunit antigen glycoprotein vaccine as immunotherapy for genital herpes in the guinea pig genital infection model","volume":"13","author":"Awasthi","year":"2017","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e00678-17","DOI":"10.1128\/mBio.00678-17","article-title":"Maternal Antiviral Immunoglobulin Accumulates in Neural Tissue of Neonates to Prevent HSV Neurological Disease","volume":"8","author":"Jiang","year":"2017","journal-title":"mBio"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1128\/JVI.80.2.929-940.2006","article-title":"Functional analysis of the triplex proteins (VP19C and VP23) of herpes simplex virus type 1","volume":"80","author":"Okoye","year":"2006","journal-title":"J. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.molcel.2007.04.010","article-title":"Allosteric signaling and a nuclear exit strategy: Binding of UL25\/UL17 heterodimers to DNA-Filled HSV-1 capsids","volume":"26","author":"Trus","year":"2007","journal-title":"Mol. Cell"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1006\/viro.2000.0298","article-title":"Immune protection against HSV-2 in B-cell-deficient mice","volume":"270","author":"Dudley","year":"2000","journal-title":"Virology"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1099\/0022-1317-82-4-845","article-title":"Differential roles of B cells and IFN-gamma-secreting CD4+ T cells in innate and adaptive immune control of genital herpes simplex virus type 2 infection in mice","volume":"82","author":"Harandi","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1099\/0022-1317-71-7-1497","article-title":"Mucosal and systemic antiviral antibodies in mice inoculated intravaginally with herpes simplex virus type 2","volume":"71","author":"Mcdermott","year":"1990","journal-title":"J. Gen. Virol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1128\/JVI.75.3.1195-1204.2001","article-title":"Vaccine-induced serum immunoglobin contributes to protection from herpes simplex virus type 2 genital infection in the presence of immune T cells","volume":"75","author":"Morrison","year":"2001","journal-title":"J. Virol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1086\/429240","article-title":"Safety and immunogenicity of glycoprotein D-adjuvant genital herpes vaccine","volume":"40","author":"Bernstein","year":"2005","journal-title":"Clin. Infect. Dis."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1586\/erv.12.129","article-title":"The challenge of developing a herpes simplex virus 2 vaccine","volume":"11","author":"Dropulic","year":"2012","journal-title":"Expert Rev. Vaccines"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1126\/science.3317824","article-title":"The T cell receptor","volume":"238","author":"Marrack","year":"1987","journal-title":"Science"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1084\/jem.169.4.1503","article-title":"H-2-linked genes influence the severity of herpes simplex virus infection of the peripheral nervous system","volume":"169","author":"Simmons","year":"1989","journal-title":"J. Exp. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1084\/jem.175.5.1337","article-title":"Anti-CD8 impairs clearance of herpes simplex virus from the nervous system: Implications for the fate of virally infected neurons","volume":"175","author":"Simmons","year":"1992","journal-title":"J. Exp. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1084\/jem.191.9.1459","article-title":"CD8+ T cells can block herpes simplex virus type 1 (HSV-1) reactivation from latency in sensory neurons","volume":"191","author":"Liu","year":"2000","journal-title":"J. Exp. Med."},{"key":"ref_69","first-page":"31","article-title":"The role of immune mechanisms in control of herpes simplex virus infection of the peripheral nervous system","volume":"179","author":"Simmons","year":"1992","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"4711","DOI":"10.1128\/jvi.69.8.4711-4716.1995","article-title":"Vaccination with recombinant vaccinia viruses expressing ICP27 induces protective immunity against herpes simplex virus through CD4+ Th1+ T cells","volume":"69","author":"Manickan","year":"1995","journal-title":"J. Virol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1089\/vim.2005.18.595","article-title":"Role of mucosal immunity in preventing genital herpes infection","volume":"18","author":"Mascasullo","year":"2005","journal-title":"Viral Immunol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1189\/jlb.0611271","article-title":"Tregs and infections: On the potential value of modifying their function","volume":"90","author":"Sehrawat","year":"2011","journal-title":"J. Leukoc. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4049\/jimmunol.1402326","article-title":"Cornea-infiltrating and lymph node dendritic cells contribute to CD4+ T cell expansion after herpes simplex virus-1 ocular infection","volume":"194","author":"Buela","year":"2015","journal-title":"J. Immunol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"7312","DOI":"10.1038\/s41467-023-43176-4","article-title":"Diatomic iron nanozyme with lipoxidase-like activity for efficient inactivation of enveloped virus","volume":"14","author":"Li","year":"2023","journal-title":"Nat. Commun."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/24\/13486\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:53:23Z","timestamp":1760115203000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/24\/13486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,16]]},"references-count":74,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["ijms252413486"],"URL":"https:\/\/doi.org\/10.3390\/ijms252413486","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,12,16]]}}}