{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:10:19Z","timestamp":1760116219815,"version":"build-2065373602"},"reference-count":62,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003336","name":"National Science Fund, Bulgaria","doi-asserted-by":"publisher","award":["DM 13-5\/2017","BG05M2OP001-1.002-0001-C04"],"award-info":[{"award-number":["DM 13-5\/2017","BG05M2OP001-1.002-0001-C04"]}],"id":[{"id":"10.13039\/501100003336","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programme \u201cScientific Research, Innovation and Digitalisation for Smart Transformation\u201d 2021-2027 (PNIIDIT)","award":["DM 13-5\/2017","BG05M2OP001-1.002-0001-C04"],"award-info":[{"award-number":["DM 13-5\/2017","BG05M2OP001-1.002-0001-C04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Der p1 is one of the major allergens causing house dust mite (HDM) allergy. Pathological Der p1-specific B cells play a key role in allergic inflammation as producers of allergen-specific antibodies. Crosslinking the inhibitory Fc\u03b3RIIb with the B cell receptor triggers a high-affinity suppressive signal in B cells. Selective elimination of allergen-specific cells could potentially be achieved by administering chimeric molecules that combine, through protein engineering, the Fc\u03b3RIIb-targeting monoclonal 2.4G2 antibody with the epitope-carrying Dp52\u201371 peptides from Der p1. We tested this hypothesis, in a chronic mouse model of HDM allergy induced in BalB\/c mice, using FACS and ELISA assays, along with histopathological and correlational analyses. Dp52\u201371chimera treatment of HDM-challenged mice led to a decrease in serum anti-HDM IgG1 antibodies, a reduction in BALF \u03b2-hexosaminidase levels, a lowered number of SiglecFhigh CD11clow eosinophils, and an improved lung PAS score. Furthermore, we observed overexpression of Fc\u03b3RIIb on the surface of CD19 cells in the lungs of HDM-challenged animals, which negatively correlated with the levels of lung alveolar macrophages, neutrophils, and BALF IL-13. Taken together, these results suggest that the use of Fc\u03b3RIIb overexpression, combined with the expansion of chimeric protein technology to include more epitopes, could improve the outcome of inflammation.<\/jats:p>","DOI":"10.3390\/ijms252413661","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T02:39:56Z","timestamp":1734921596000},"page":"13661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Suppression of Pathological Allergen-Specific B Cells by Protein-Engineered Molecules in a Mouse Model of Chronic House Dust Mite Allergy"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-5914","authenticated-orcid":false,"given":"Nikola","family":"Ralchev","sequence":"first","affiliation":[{"name":"Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silviya","family":"Bradyanova","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikola","family":"Kerekov","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey","family":"Tchorbanov","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8582-9296","authenticated-orcid":false,"given":"Nikolina","family":"Mihaylova","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.molimm.2023.04.008","article-title":"House dust mite allergy: The importance of house dust mite allergens for diagnosis and immunotherapy","volume":"158","author":"Huang","year":"2023","journal-title":"Mol. Immunol."},{"key":"ref_2","first-page":"38","article-title":"Respiratory allergy caused by house dust mites: What do we really know?","volume":"136","author":"Linneberg","year":"2014","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s40413-017-0145-4","article-title":"International consensus (ICON) on: Clinical consequences of mite hypersensitivity, a global problem","volume":"10","author":"Thomas","year":"2017","journal-title":"World Allergy Organ. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/j.jaip.2015.06.019","article-title":"House Dust Mite Respiratory Allergy: An Overview of Current Therapeutic Strategies","volume":"3","author":"Linneberg","year":"2015","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"24","DOI":"10.23937\/2572-3308.1510024","article-title":"Review: Side Effects of Some Commonly Used Allergy Medications (Decongestants, Anti-Leukotriene Agents, Antihistamines, Steroids, and Zinc) and Their Safety in Pregnancy","volume":"3","author":"Malone","year":"2017","journal-title":"Int. J. Allergy Medicat."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1517\/14712598.2013.844226","article-title":"Allergen immunotherapy for house dust mite: Clinical efficacy and immunological mechanisms in allergic rhinitis and asthma","volume":"13","author":"Eifan","year":"2013","journal-title":"Expert Opin. Biol. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"107125","DOI":"10.1016\/j.rmed.2023.107125","article-title":"Allergen immunotherapy for allergic asthma: The future seems bright","volume":"210","author":"Diamant","year":"2023","journal-title":"Respir. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"101334","DOI":"10.1016\/j.smim.2019.101334","article-title":"Allergen immunotherapy (AIT) in asthma","volume":"46","author":"Virchow","year":"2019","journal-title":"Semin. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s40521-019-00236-9","article-title":"House-Dust Mite Immunotherapy in Asthma: Uncertainties and Therapeutic Strategies","volume":"6","author":"Liew","year":"2019","journal-title":"Curr. Treat. Options Allergy"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1111\/all.14639","article-title":"Roles of follicular helper and regulatory T cells in allergic diseases and allergen immunotherapy","volume":"76","author":"Yao","year":"2020","journal-title":"Allergy"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/imr.12555","article-title":"Mechanisms of immune regulation in allergic diseases: The role of regulatory T and B cells","volume":"278","author":"Palomares","year":"2017","journal-title":"Immunol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.alit.2020.01.004","article-title":"Roles of omalizumab in various allergic diseases","volume":"69","author":"Okayama","year":"2020","journal-title":"Allergol. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/2049-6958-9-23","article-title":"Treating severe allergic asthma with anti-IgE monoclonal antibody (omalizumab): A review","volume":"9","author":"Stanziola","year":"2014","journal-title":"Multidiscip. Respir. Med."},{"doi-asserted-by":"crossref","unstructured":"Waldron, R., McGowan, J., Gordon, N., McCarthy, C., Mitchell, E.B., and Fitzpatrick, D.A. (2019). Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus. PLoS ONE, 14.","key":"ref_14","DOI":"10.1371\/journal.pone.0216171"},{"doi-asserted-by":"crossref","unstructured":"Reithofer, M., and Jahn-Schmid, B. (2017). Allergens with Protease Activity from House Dust Mites. Int. J. Mol. Sci., 18.","key":"ref_15","DOI":"10.3390\/ijms18071368"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3390\/allergies1020008","article-title":"House Dust Mite Allergy and the Der p1 Conundrum: A Literature Review and Case Series","volume":"1","author":"Bergantini","year":"2021","journal-title":"Allergies"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"307","DOI":"10.4049\/jimmunol.1402199","article-title":"Structural Analysis of Der p 1\u2013Antibody Complexes and Comparison with Complexes of Proteins or Peptides with Monoclonal Antibodies","volume":"195","author":"Osinski","year":"2015","journal-title":"J. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1007\/s12016-013-8396-5","article-title":"Immunoglobulin E-Binding Epitopes of Mite Allergens: From Characterization to Immunotherapy","volume":"47","author":"Cui","year":"2013","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_19","first-page":"453","article-title":"Modulation of the allergen-induced human ige response in hu-scid mice: Inhibitory effect of human recombinant ifn-\u03b3 and allergen-derived lipopeptide","volume":"12","author":"Duez","year":"2001","journal-title":"Eur. Cytokine Netw."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1365-2249.1993.tb05959.x","article-title":"Comparative analysis of biological activities of Der p I-derived peptides on Fc receptor-bearing cells from Dermatophagoides pteronyssinus-sensitive patients","volume":"92","author":"Jeannin","year":"1993","journal-title":"Clin. Exp. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.imbio.2013.07.009","article-title":"Suppression of allergen-specific B lymphocytes by chimeric protein-engineered antibodies","volume":"219","author":"Kerekov","year":"2014","journal-title":"Immunobiology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e13241","DOI":"10.1111\/sji.13241","article-title":"Targeted suppression of Dpt-specific B cells in humanized Rag2- \u03b3c- mouse model of HDM allergy","volume":"97","author":"Ralchev","year":"2022","journal-title":"Scand. J. Immunol."},{"doi-asserted-by":"crossref","unstructured":"Chen, B., Liu, H., Liu, Z., and Yang, F. (2023). Benefits and limitations of humanized mouse models for human red blood cell-related disease research. Front. Hematol., 1.","key":"ref_23","DOI":"10.3389\/frhem.2022.1062705"},{"doi-asserted-by":"crossref","unstructured":"Ito, R., Maruoka, S., Gon, Y., Katano, I., Takahashi, T., Ito, M., Izuhara, K., and Nunomura, S. (2019). Recent Advances in Allergy Research Using Humanized Mice. Int. J. Mol. Sci., 20.","key":"ref_24","DOI":"10.3390\/ijms20112740"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s40259-018-0275-4","article-title":"Humanized Mouse Models for the Preclinical Assessment of Cancer Immunotherapy","volume":"32","author":"Wege","year":"2018","journal-title":"BioDrugs"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.imlet.2021.06.006","article-title":"New aspects in the regulation of human B cell functions by complement receptors CR1, CR2, CR3 and CR4","volume":"237","author":"Erdei","year":"2021","journal-title":"Immunol. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e157250","DOI":"10.1172\/JCI157250","article-title":"Fc\u03b3RIIB regulates autoantibody responses by limiting marginal zone B cell activation","volume":"132","author":"Barlev","year":"2022","journal-title":"J. Clin. Investig."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.jaut.2012.04.006","article-title":"Immune complex-mediated co-ligation of the BCR with Fc\u03b3RIIB results in homeostatic apoptosis of B cells involving Fas signalling that is defective in the MRL\/Lpr model of systemic lupus erythematosus","volume":"39","author":"Paunovic","year":"2012","journal-title":"J. Autoimmun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1002\/eji.200737143","article-title":"Selective silencing of DNA-specific B lymphocytes delays lupus activity in MRL\/lpr mice","volume":"37","author":"Tchorbanov","year":"2007","journal-title":"Eur. J. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.molimm.2009.01.013","article-title":"Simultaneous engagement of Fc\u03b3IIb and CD22 inhibitory receptors silences targeted B cells and suppresses autoimmune disease activity","volume":"47","author":"Mihaylova","year":"2009","journal-title":"Mol. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12931-019-1136-5","article-title":"Complement factor C5 inhibition reduces type 2 responses without affecting group 2 innate lymphoid cells in a house dust mite induced murine asthma model","volume":"20","author":"Yang","year":"2019","journal-title":"Respir. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"112136","DOI":"10.1016\/j.jep.2019.112136","article-title":"Total glucosides of peony improve ovalbumin-induced allergic asthma by inhibiting mast cell degranulation","volume":"244","author":"Shou","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/all.12776","article-title":"Phenotypic plasticity and targeting of Siglec-FhighCD11clow eosinophils to the airway in a murine model of asthma","volume":"71","author":"Valencia","year":"2015","journal-title":"Allergy"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1002\/JLB.3HI0821-432RR","article-title":"Modulation of surface CD11c expression tracks plasticity in murine intestinal tissue eosinophils","volume":"111","author":"Larsen","year":"2022","journal-title":"J. Leukoc. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.290.5489.84","article-title":"Immune Inhibitory Receptors","volume":"290","author":"Ravetch","year":"2000","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/S1074-7613(00)80074-6","article-title":"SHIP Recruitment Attenuates Fc\u03b3RIIB-Induced B Cell Apoptosis","volume":"10","author":"Pearse","year":"1999","journal-title":"Immunity"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1136\/thoraxjnl-2011-200606","article-title":"The composition of house dust mite is critical for mucosal barrier dysfunction and allergic sensitisation","volume":"67","author":"Post","year":"2011","journal-title":"Thorax"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1038\/nri2762","article-title":"Fc\u03b3RIIB in autoimmunity and infection: Evolutionary and therapeutic implications","volume":"10","author":"Smith","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1586\/eci.12.5","article-title":"Fc\u03b3RIIB: A modulator of cell activation and humoral tolerance","volume":"8","author":"Lehmann","year":"2012","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1183\/09031936.00069110","article-title":"Do the current house dust mite-driven models really mimic allergic asthma?","volume":"36","author":"Birrell","year":"2010","journal-title":"Eur. Respir. J."},{"key":"ref_41","first-page":"e90420","article-title":"Lung CD103+ dendritic cells restrain allergic airway inflammation through IL-12 production","volume":"2","author":"Conejero","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1164\/rccm.200308-1094OC","article-title":"Continuous Exposure to House Dust Mite Elicits Chronic Airway Inflammation and Structural Remodeling","volume":"169","author":"Johnson","year":"2004","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/all.12094","article-title":"Farm-derived Gram-positive bacterium Staphylococcus sciuri W620 prevents asthma phenotype in HDM- and OVA-exposed mice","volume":"68","author":"Hagner","year":"2013","journal-title":"Allergy"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1111\/cea.12388","article-title":"Enhanced allergic airway disease in old mice is associated with a Th17 response","volume":"44","author":"Brandenberger","year":"2014","journal-title":"Clin. Exp. Allergy"},{"doi-asserted-by":"crossref","unstructured":"Post, S., Heijink, I.H., Petersen, A.H., de Bruin, H.G., van Oosterhout, A.J.M., and Nawijn, M.C. (2014). Protease-Activated Receptor-2 Activation Contributes to House Dust Mite-Induced IgE Responses in Mice. PLoS ONE, 9.","key":"ref_45","DOI":"10.1371\/journal.pone.0091206"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1165\/rcmb.2016-0356OC","article-title":"House Dust Mite\u2013Induced Allergic Airway Disease Is Independent of IgE and Fc\u03b5RI\u03b1","volume":"57","author":"McKnight","year":"2017","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1172\/JCI118560","article-title":"Passive transfer of immediate hypersensitivity and airway hyperresponsiveness by allergen-specific immunoglobulin (Ig) E and IgG1 in mice","volume":"97","author":"Oshiba","year":"1996","journal-title":"J. Clin. Investig."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1172\/JCI119255","article-title":"Systemic anaphylaxis in the mouse can be mediated largely through IgG1 and Fc gammaRIII. Assessment of the cardiopulmonary changes, mast cell degranulation, and death associated with active or IgE- or IgG1-dependent passive anaphylaxis","volume":"99","author":"Miyajima","year":"1997","journal-title":"J. Clin. Investig."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S1074-7613(00)80494-X","article-title":"Impaired IgG-Dependent Anaphylaxis and Arthus Reaction in Fc\u03b3RIII (CD16) Deficient Mice","volume":"5","author":"Hazenbos","year":"1996","journal-title":"Immunity"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1002\/eji.200939900","article-title":"Fc\u03b3 receptor-mediated antigen uptake by lung DC contributes to allergic airway hyper-responsiveness and inflammation","volume":"40","author":"Hartwig","year":"2010","journal-title":"Eur. J. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"236075","DOI":"10.1155\/2012\/236075","article-title":"The Contribution of Allergen-Specific IgG to the Development of Th2-Mediated Airway Inflammation","volume":"2012","author":"Williams","year":"2012","journal-title":"J. Allergy"},{"doi-asserted-by":"crossref","unstructured":"Victor, J.R., Muniz, B.P., E Fusaro, A., A de Brito, C., Taniguchi, E.F., Duarte, A.J., and Sato, M.N. (2010). Maternal immunization with ovalbumin prevents neonatal allergy development and up-regulates inhibitory receptor Fc\u03b3RIIB expression on B cells. BMC Immunol., 11.","key":"ref_52","DOI":"10.1186\/1471-2172-11-11"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13223-017-0195-8","article-title":"Preconception allergen sensitization can induce B10 cells in offspring: A potential main role for maternal IgG","volume":"13","author":"Oliveira","year":"2017","journal-title":"Allergy Asthma Clin. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1710-1492-10-47","article-title":"Maternal immunization with ovalbumin or Dermatophagoides pteronyssinus has opposing effects on Fc\u03b3RIIb expression on offspring B cells","volume":"10","author":"Lira","year":"2014","journal-title":"Allergy, Asthma Clin. Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jaci.2017.03.045","article-title":"Allergen-specific IgG antibody signaling through Fc\u03b3RIIb promotes food tolerance","volume":"141","author":"Burton","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"doi-asserted-by":"crossref","unstructured":"Gomez, G. (2019). Current Strategies to Inhibit High Affinity Fc\u03b5RI-Mediated Signaling for the Treatment of Allergic Disease. Front. Immunol., 10.","key":"ref_56","DOI":"10.3389\/fimmu.2019.00175"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/j.coi.2005.09.012","article-title":"Regulation of allergy by Fc receptors","volume":"17","author":"Bruhns","year":"2005","journal-title":"Curr. Opin. Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4412","DOI":"10.4049\/jimmunol.1301324","article-title":"Important Role for Fc\u03b3RIIB on B Lymphocytes for Mucosal Antigen-Induced Tolerance and Foxp3+ Regulatory T Cells","volume":"191","author":"Sun","year":"2013","journal-title":"J. Immunol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.imlet.2007.12.016","article-title":"An antibody-based construct carrying DNA-mimotope and targeting CR1(CD35) selectively suppresses human autoreactive B-lymphocytes","volume":"116","author":"Voynova","year":"2008","journal-title":"Immunol. Lett."},{"key":"ref_60","first-page":"S49","article-title":"Limit of Blank, Limit of Detection and Limit of Quantitation","volume":"29","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev."},{"doi-asserted-by":"crossref","unstructured":"Kujur, W., Gurram, R.K., Haleem, N., Maurya, S.K., and Agrewala, J.N. (2015). Caerulomycin A inhibits Th2 cell activity: A possible role in the management of asthma. Sci. Rep., 5.","key":"ref_61","DOI":"10.1038\/srep15396"},{"doi-asserted-by":"crossref","unstructured":"Tang, D., Chen, M., Huang, X., Zhang, G., Zeng, L., Zhang, G., Wu, S., and Wang, Y. (2023). SRplot: A free online platform for data visualization and graphing. PLoS ONE, 18.","key":"ref_62","DOI":"10.1371\/journal.pone.0294236"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/24\/13661\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:57:07Z","timestamp":1760115427000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/24\/13661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,20]]},"references-count":62,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["ijms252413661"],"URL":"https:\/\/doi.org\/10.3390\/ijms252413661","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,12,20]]}}}