{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T15:40:36Z","timestamp":1766504436583,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T00:00:00Z","timestamp":1734998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["AP229\/2-1"],"award-info":[{"award-number":["AP229\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Nitric oxide-sensitive guanylyl cyclase (NO-GC) is a heterodimeric enzyme with an \u03b1- and a \u03b2-subunit. In its active form as an \u03b11\u03b21-heterodimer, NO-GC produces cyclic guanosine-3\u2032,5\u2032-monophophate (cGMP) to regulate vasodilation and proliferation of vascular smooth muscle cells (VSMCs). In contrast to VSMCs, only a few studies reported on the expression of the NO-GC \u03b11\u03b21-heterodimer in human pericytes. Since NO-GC is a marker for platelet-derived growth factor-\u03b2 (PDGFR\u03b2)-positive pericytes, we investigated whether NO-GC is expressed in its active \u03b11\u03b21-heterodimer in pericytes of healthy human dental pulp. In our previous studies, we developed and validated an antibody against the \u03b11-subunit of human NO-GC. Here, we developed a new antibody against the \u03b21-subunit of human NO-GC and validated it by immunoblot, mass spectrometry, and immunohistochemistry on tissue samples from humans and NO-GC knockout (GCKO) mice. Using both antibodies, we detected \u03b11- and \u03b21-subunits of NO-GC in pericytes of pre-capillary arterioles, capillaries, and post-capillary venules in dental pulp of decalcified and non-decalcified human molars. We concluded that NO-GC as an active \u03b11\u03b21-heterodimer may be involved in the regulation of vascular permeability, vascular stability, organ homeostasis, and organ regeneration in healthy human dental pulp.<\/jats:p>","DOI":"10.3390\/ijms26010030","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T09:00:57Z","timestamp":1735030857000},"page":"30","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The \u03b11- and \u03b21-Subunits of Nitric Oxide-Sensitive Guanylyl Cyclase in Pericytes of Healthy Human Dental Pulp"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2670-9942","authenticated-orcid":false,"given":"Y\u00fcksel","family":"Korkmaz","sequence":"first","affiliation":[{"name":"Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galyna","family":"Pryymachuk","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Brandenburg Medical School Theodor Fontane, 14770 Brandenburg an der Havel, Germany"},{"name":"Department of Anatomy I, University of Cologne, 50937 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0184-1146","authenticated-orcid":false,"given":"Mechthild M.","family":"Schroeter","sequence":"additional","affiliation":[{"name":"Center for Physiology and Pathophysiology Faculty of Medicine and University Hospital Cologne, 51109 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5909-6105","authenticated-orcid":false,"given":"Behrus","family":"Puladi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, University Hospital RWTH Aachen, RWTH Aachen University, 52074 Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadin","family":"Piekarek","sequence":"additional","affiliation":[{"name":"Experimental Medicine, Faculty of Medicine and University Hospital Cologne, University of Cologne, 51109 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0315-6513","authenticated-orcid":false,"given":"Sarah","family":"Appel","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50937 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelm","family":"Bloch","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Sport Medicine, German Sport University Cologne, 50933 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8182-8034","authenticated-orcid":false,"given":"Jan-Wilm","family":"Lackmann","sequence":"additional","affiliation":[{"name":"Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases, University of Cologne, 50931 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Deschner","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8178-3948","authenticated-orcid":false,"given":"Andreas","family":"Friebe","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of W\u00fcrzburg, 97070 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1056\/NEJMsa063904","article-title":"Shattuck Lecture. 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