{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:45:12Z","timestamp":1793033112375,"version":"4.5.0"},"reference-count":104,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T00:00:00Z","timestamp":1735084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["075-10-2021-113"],"award-info":[{"award-number":["075-10-2021-113"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The black garden ant (Lasius niger) is a widely distributed species across Europe, North America, and North Africa, playing a pivotal role in ecological processes within its diverse habitats. However, the microbiome associated with L. niger remains poorly investigated. In the present study, we isolated a novel species, Paenarthrobacter lasiusi, from the soil of the L. niger anthill. The genome of P. lasiusi S21 was sequenced, annotated, and searched for groups of genes of physiological, medical, and biotechnological importance. Subsequently, a series of microbiological, physiological, and biochemical experiments were conducted to characterize P. lasiusi S21 with respect to its sugar metabolism, antibiotic resistance profile, lipidome, and capacity for atmospheric nitrogen fixation, among others. A notable feature of the P. lasiusi S21 genome is the presence of two prophages, which may have horizontally transferred host genes involved in stress responses. P. lasiusi S21 synthesizes a number of lipids, including mono- and digalactosyldiacylglycerol, as well as steroid compounds that are typically found in eukaryotic organisms rather than prokaryotes. P. lasiusi S21 exhibits resistance to penicillins, lincosamides, fusidins, and oxazolidinones, despite the absence of specific genes conferring resistance to these antibiotics. Genomic data and physiological tests indicate that P. lasiusi S21 is nonpathogenic to humans. The genome of P. lasiusi S21 contains multiple operons involved in heavy metal metabolism and organic compound inactivation. Consequently, P. lasiusi represents a novel species with an intriguing evolutionary history, manifesting in distinctive genomic, metabolomic, and physiological characteristics. This species may have potential applications in the bioaugmentation of contaminated soils.<\/jats:p>","DOI":"10.3390\/ijms26010067","type":"journal-article","created":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T19:29:52Z","timestamp":1735154992000},"page":"67","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unusual Genomic and Biochemical Features of Paenarthrobacter lasiusi sp. nov\u2014A Novel Bacterial Species Isolated from Lasius niger Anthill Soil"],"prefix":"10.3390","volume":"26","author":[{"given":"Alexandra A.","family":"Dymova","sequence":"first","affiliation":[{"name":"Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia"},{"name":"Faculty of Soil Science, M.V. Lomonosov Moscow State University, Leninskie Gory, 1, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5565-1331","authenticated-orcid":false,"given":"Maxim A.","family":"Kovalev","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artemiy S.","family":"Silantyev","sequence":"additional","affiliation":[{"name":"The Institute of Personalized Cardiology, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3763-5953","authenticated-orcid":false,"given":"Anna A.","family":"Borzykh","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamila J.","family":"Osipova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4926-8289","authenticated-orcid":false,"given":"Svetlana V.","family":"Poddubko","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-1983","authenticated-orcid":false,"given":"Vladimir A.","family":"Mitkevich","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5203-0787","authenticated-orcid":false,"given":"Dmitry S.","family":"Karpov","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia"},{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"},{"name":"Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0884-6775","authenticated-orcid":false,"given":"Natalia V.","family":"Kostina","sequence":"additional","affiliation":[{"name":"Faculty of Soil Science, M.V. Lomonosov Moscow State University, Leninskie Gory, 1, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev-ecolsys-120213-091824","article-title":"The Phylogeny and Evolution of Ants","volume":"45","author":"Ward","year":"2014","journal-title":"Annu. Rev. Ecol. Evol. Syst."},{"key":"ref_2","first-page":"28","article-title":"Ants of Wyre Forest\u2014A Review","volume":"10","author":"Green","year":"2009","journal-title":"Wyre For. 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