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The pathogenesis of ALD involves a complex interplay of various molecular, biochemical, genetic, epigenetic, and environmental factors. While the mechanisms are well studied, therapeutic options remain limited. Alpinetin, a natural flavonoid with antioxidant and anti-inflammatory properties, has shown potential hepatoprotective effects, though its efficacy in ALD remains unexplored. This study investigated the hepatoprotective effects of alpinetin using a Lieber\u2013DeCarli ethanol liquid diet model of ALD in C57BL\/6 mice. Mice were divided into three groups: the control group, the ethanol group, and the ethanol group treated with alpinetin. Serum activity of ALT, AST, \u03b3-GT, and ALP was measured to assess liver function, along with antioxidative and oxidative\/nitrosative stress markers in liver tissue. Pro-inflammatory cytokines and endoplasmic reticulum (ER) stress parameters in liver tissue were also evaluated. Histological assessment of disease activity was performed using the SALVE grading and staging system. Treatment with alpinetin significantly reduced serum levels of ALT, AST, \u03b3-GT, and oxidative\/nitrosative stress markers while increasing antioxidative markers. The levels of pro-inflammatory cytokines and ER stress parameters were significantly decreased. Histological analysis demonstrated reduced steatosis, hepatocyte ballooning, and inflammation. These findings suggest that alpinetin holds promise as a potential therapeutic agent for managing ALD.<\/jats:p>","DOI":"10.3390\/ijms26010086","type":"journal-article","created":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T22:00:58Z","timestamp":1735164058000},"page":"86","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Alpinetin Exhibits Antioxidant and Anti-Inflammatory Effects in C57BL\/6 Mice with Alcoholic Liver Disease Induced by the Lieber\u2013DeCarli Ethanol Liquid Diet"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1701-3313","authenticated-orcid":false,"given":"Tatjana","family":"Radosavljevic","sequence":"first","affiliation":[{"name":"Institute of Pathophysiology \u201cLjubodrag Buba Mihailovi\u0107\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Brankovic","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8457-4962","authenticated-orcid":false,"given":"Jasmina","family":"Djureti\u0107","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8014-3121","authenticated-orcid":false,"given":"Jelica","family":"Grujic-Milanovic","sequence":"additional","affiliation":[{"name":"Institute for Medical Research, National Institute of the Republic of Serbia, Department of Cardiovascular Research, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijana","family":"Kovacic","sequence":"additional","affiliation":[{"name":"Group of Immunology, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3424-4623","authenticated-orcid":false,"given":"Jovan","family":"Jevtic","sequence":"additional","affiliation":[{"name":"Institute of Pathology \u2018Dr \u0110or\u0111e Joannovi\u0107\u2019, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0890-535X","authenticated-orcid":false,"given":"Sanja","family":"Stankovic","sequence":"additional","affiliation":[{"name":"Centre for Medical Biochemistry, University Clinical Centre of Serbia, 11000 Belgrade, Serbia"},{"name":"Department of Biochemistry, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8464-4924","authenticated-orcid":false,"given":"Janko","family":"Samardzic","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danijela","family":"Vucevic","sequence":"additional","affiliation":[{"name":"Institute of Pathophysiology \u201cLjubodrag Buba Mihailovi\u0107\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Jakovljevic","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia"},{"name":"Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, 34000 Kragujevac, Serbia"},{"name":"Department of Human Pathology, First Moscow State Medical University I.M. 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