{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:41:56Z","timestamp":1760118116114,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["1021062812154-3-3.2.2"],"award-info":[{"award-number":["1021062812154-3-3.2.2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the present study, we aimed to investigate intratumoral karyotype diversity as well as the estrogen\/progesterone effect on the cytogenetic profile of uterine leiomyomas (ULs). A total of 15 UL samples obtained from 15 patients were cultured in the media supplemented with estrogen and\/or progesterone and without adding hormones. Conventional cytogenetic analysis of culture samples revealed clonal chromosomal abnormalities in 11 out of 15 ULs. Cytogenetic findings were presented by simple and complex chromosomal rearrangements (64% and 36% of cases, respectively) verified through FISH and aCGH. In most ULs with complex chromosomal rearrangements, the breakpoints did not feature clusterization on a single chromosome but were evenly distributed across rearranged chromosomes. The number of breakpoints showed a strong positive correlation with the number of rearranged chromosomes. Moreover, both abovementioned parameters were in a linear dependency from the number of karyotypically different clones per UL. This suggests that complex chromosomal rearrangements in ULs predominantly originate through sequential events rather than one hit. The results of UL cytogenetic analysis depended on the presence of estrogen and\/or progesterone in the culture medium. The greatest variety of cytogenetically different cell clones was detected in the samples cultured without hormone supplementation. Their counterparts cultured with progesterone supplementation showed a sharp decrease in clone number, whereas such a decrease induced by estrogen or estrogen\u2013progesterone supplementation was insignificant. These findings suggest that estrogen\u2013progesterone balance is crucial for forming a UL cytogenetic profile, which, in turn, may underlie the unique response of the every karyotypically abnormal UL to medications.<\/jats:p>","DOI":"10.3390\/ijms26010096","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T03:10:49Z","timestamp":1735182649000},"page":"96","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Effect of Estrogen and Progesterone on Cytogenetic Profile of Uterine Leiomyomas"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6587-9429","authenticated-orcid":false,"given":"Alla S.","family":"Koltsova","sequence":"first","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna A.","family":"Pendina","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8626-5071","authenticated-orcid":false,"given":"Olga V.","family":"Malysheva","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6529-5799","authenticated-orcid":false,"given":"Ekaterina D.","family":"Trusova","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9716-4964","authenticated-orcid":false,"given":"Dmitrii A.","family":"Staroverov","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6551-4147","authenticated-orcid":false,"given":"Maria I.","family":"Yarmolinskaya","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolai I.","family":"Polenov","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7465-4504","authenticated-orcid":false,"given":"Andrey S.","family":"Glotov","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor Yu.","family":"Kogan","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4495-0983","authenticated-orcid":false,"given":"Olga A.","family":"Efimova","sequence":"additional","affiliation":[{"name":"D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034 St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Koltsova, A.S., Efimova, O.A., and Pendina, A.A. (2023). A View on Uterine Leiomyoma Genesis through the Prism of Genetic, Epigenetic and Cellular Heterogeneity. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065752"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1210\/endrev\/bnab039","article-title":"Comprehensive Review of Uterine Fibroids: Developmental Origin, Pathogenesis, and Treatment","volume":"43","author":"Yang","year":"2022","journal-title":"Endocr. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1093\/humupd\/dmv030","article-title":"Epidemiological and Genetic Clues for Molecular Mechanisms Involved in Uterine Leiomyoma Development and Growth","volume":"21","author":"Commandeur","year":"2015","journal-title":"Hum. Reprod. Update"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/ijgo.13102","article-title":"Epidemiology and Management of Uterine Fibroids","volume":"149","author":"Giuliani","year":"2020","journal-title":"Int. J. Gynaecol. Obstet. Off. Organ Int. Fed. Gynaecol. Obstet."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Baranov, V.S., Osinovskaya, N.S., and Yarmolinskaya, M.I. (2019). Pathogenomics of Uterine Fibroids Development. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20246151"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1136\/jclinpath-2016-203976","article-title":"Catechol-O-Methyltransferase Val158Met Polymorphism Is Associated with Increased Risk of Multiple Uterine Leiomyomas Either Positive or Negative for MED12 Exon 2 Mutations","volume":"70","author":"Dzhemlikhanova","year":"2017","journal-title":"J. Clin. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1097\/MED.0000000000000578","article-title":"The Role of Endocrine-Disrupting Chemicals in Uterine Fibroid Pathogenesis","volume":"27","author":"Bariani","year":"2020","journal-title":"Curr. Opin. Endocrinol. Diabetes Obes."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"W\u0142odarczyk, M., Nowicka, G., Ciebiera, M., Ali, M., Yang, Q., and Al-Hendy, A. (2022). Epigenetic Regulation in Uterine Fibroids-The Role of Ten-Eleven Translocation Enzymes and Their Potential Therapeutic Application. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052720"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"495","DOI":"10.2147\/IJWH.S211632","article-title":"LncRNA SRA1 May Play a Role in the Uterine Leiomyoma Tumor Growth Regarding the MED12 Mutation Pattern","volume":"11","author":"Akbari","year":"2019","journal-title":"Int. J. Women\u2019s Health"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1016\/j.fertnstert.2014.06.040","article-title":"MED12 Mutation Frequency in Unselected Sporadic Uterine Leiomyomas","volume":"102","author":"Heinonen","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1097\/PGP.0000000000000255","article-title":"Frequency and Spectrum of MED12 Exon 2 Mutations in Multiple Versus Solitary Uterine Leiomyomas from Russian Patients","volume":"35","author":"Osinovskaya","year":"2016","journal-title":"Int. J. Gynecol. Pathol. Off. J. Int. Soc. Gynecol. Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.fertnstert.2020.05.036","article-title":"HMGA2-Mediated Tumorigenesis through Angiogenesis in Leiomyoma","volume":"114","author":"Li","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cancergencyto.2004.08.025","article-title":"Updates on the Cytogenetics and Molecular Genetics of Bone and Soft Tissue Tumors: Leiomyoma","volume":"158","author":"Sandberg","year":"2005","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.ejogrb.2017.10.028","article-title":"Case of Chromothripsis in a Large Solitary Non-Recurrent Uterine Leiomyoma","volume":"219","author":"Pendina","year":"2017","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s13039-014-0088-1","article-title":"Cytogenetically Normal Uterine Leiomyomas without MED12-Mutations\u2014A Source to Identify Unknown Mechanisms of the Development of Uterine Smooth Muscle Tumors","volume":"7","author":"Holzmann","year":"2014","journal-title":"Mol. Cytogenet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1056\/NEJMoa1302736","article-title":"Characterization of Uterine Leiomyomas by Whole-Genome Sequencing","volume":"369","author":"Mehine","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Koltsova, A.S., Efimova, O.A., Malysheva, O.V., Osinovskaya, N.S., Liehr, T., Al-Rikabi, A., Shved, N.Y., Sultanov, I.Y., Chiryaeva, O.G., and Yarmolinskaya, M.I. (2021). Cytogenomic Profile of Uterine Leiomyoma: In Vivo vs. In Vitro Comparison. Biomedicines, 9.","DOI":"10.3390\/biomedicines9121777"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Koltsova, A.S., Pendina, A.A., Efimova, O.A., Chiryaeva, O.G., Kuznetzova, T.V., and Baranov, V.S. (2019). On the Complexity of Mechanisms and Consequences of Chromothripsis: An Update. Front. Genet., 10.","DOI":"10.3389\/fgene.2019.00393"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"572.e1","DOI":"10.1016\/j.ajog.2014.01.011","article-title":"Uterine Cellular Leiomyomata with Chromosome 1p Deletions Represent a Distinct Entity","volume":"210","author":"Hodge","year":"2014","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1093\/molehr\/5.12.1150","article-title":"Chromosomal Translocations Affecting 12q14-15 but Not Deletions of the Long Arm of Chromosome 7 Associated with a Growth Advantage of Uterine Smooth Muscle Cells","volume":"5","author":"Hennig","year":"1999","journal-title":"Mol. Hum. Reprod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s13000-019-0809-1","article-title":"Leiomyoma with KAT6B-KANSL1 Fusion: Case Report of a Rapidly Enlarging Uterine Mass in a Postmenopausal Woman","volume":"14","author":"Ainsworth","year":"2019","journal-title":"Diagn. Pathol."},{"key":"ref_22","first-page":"1259","article-title":"Association of the Shrinkage of Uterine Leiomyoma Treated with GnRH Agonist and Deletion of Long Arm of Chromosome 7","volume":"18","author":"Takahashi","year":"2001","journal-title":"Int. J. Oncol."},{"key":"ref_23","first-page":"279","article-title":"Shrinkage Effect of Gonadotropin Releasing Hormone Agonist Treatment on Uterine Leiomyomas and t(12;14)","volume":"20","author":"Takahashi","year":"2002","journal-title":"Int. J. Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1093\/hmg\/ddac217","article-title":"Molecular Subclass of Uterine Fibroids Predicts Tumor Shrinkage in Response to Ulipristal Acetate","volume":"32","author":"Kolterud","year":"2022","journal-title":"Hum. Mol. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1093\/humrep\/deac183","article-title":"Single-Cell Sequencing Reveals Novel Cellular Heterogeneity in Uterine Leiomyomas","volume":"37","author":"Goad","year":"2022","journal-title":"Hum. Reprod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/gcc.2870110102","article-title":"Analysis of Androgen Receptor DNA Reveals the Independent Clonal Origins of Uterine Leiomyomata and the Secondary Nature of Cytogenetic Aberrations in the Development of Leiomyomata","volume":"11","author":"Mashal","year":"1994","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1159\/000513173","article-title":"Uterine Leiomyomas with an Apparently Normal Karyotype Comprise Minor Heteroploid Subpopulations Differently Represented in Vivo and in Vitro","volume":"161","author":"Koltsova","year":"2021","journal-title":"Cytogenet. Genome Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1002\/gcc.2870020409","article-title":"Parallel Karyotypic Evolution and Tumor Progression in Uterine Leiomyoma","volume":"2","author":"Pandis","year":"1990","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0165-4608(93)90045-N","article-title":"Chromosome 7 Biclonality in Uterine Leiomyoma","volume":"67","author":"Ozisik","year":"1993","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1093\/hmg\/ddv177","article-title":"Clonally Related Uterine Leiomyomas Are Common and Display Branched Tumor Evolution","volume":"24","author":"Mehine","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"S251","DOI":"10.1016\/j.jor.2015.10.016","article-title":"An Unusual Karyotype in Leiomyoma: Case Report and Literature Review","volume":"12","author":"Chang","year":"2015","journal-title":"J. Orthop."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0165-4608(88)90143-4","article-title":"Ring Formation and Structural Rearrangements of Chromosome 1 as Secondary Changes in Uterine Leiomyomas with t(12;14)(Q14-15;Q23-24)","volume":"36","author":"Nilbert","year":"1988","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1038\/modpathol.2015.3","article-title":"Uterine Smooth Muscle Tumor Analysis by Comparative Genomic Hybridization: A Useful Diagnostic Tool in Challenging Lesions","volume":"28","author":"Croce","year":"2015","journal-title":"Mod. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/BF00193583","article-title":"Characteristic Chromosome Abnormalities, Including Rearrangements of 6p, Del(7q), +12, and t(12;14), in 44 Uterine Leiomyomas","volume":"85","author":"Nilbert","year":"1990","journal-title":"Hum. Genet."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/0046-8177(91)90009-E","article-title":"Subgroups of Uterine Leiomyomas Based on Cytogenetic Analysis","volume":"22","author":"Hu","year":"1991","journal-title":"Hum. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0165-4608(91)90229-N","article-title":"Chromosome Analysis of 96 Uterine Leiomyomas","volume":"55","author":"Pandis","year":"1991","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0165-4608(96)00406-2","article-title":"The Del(7q) Subgroup in Uterine Leiomyomata: Genetic and Biologic Characteristics. Further Evidence for the Secondary Nature of Cytogenetic Abnormalities in the Pathobiology of Uterine Leiomyomata","volume":"98","author":"Xing","year":"1997","journal-title":"Cancer Genet. Cytogenet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1159\/000132682","article-title":"Karyotypic Rearrangements in 20 Uterine Leiomyomas","volume":"49","author":"Nilbert","year":"1988","journal-title":"Cytogenet. Cell Genet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1016\/j.cell.2013.03.021","article-title":"Punctuated Evolution of Prostate Cancer Genomes","volume":"153","author":"Baca","year":"2013","journal-title":"Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/s43018-023-00711-y","article-title":"Aneuploidy and Complex Genomic Rearrangements in Cancer Evolution","volume":"5","author":"Baker","year":"2024","journal-title":"Nat. Cancer"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e2025182118","DOI":"10.1073\/pnas.2025182118","article-title":"Integrated Mutational Landscape Analysis of Uterine Leiomyosarcomas","volume":"118","author":"Choi","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.cell.2010.11.055","article-title":"Massive Genomic Rearrangement Acquired in a Single Catastrophic Event during Cancer Development","volume":"144","author":"Stephens","year":"2011","journal-title":"Cell"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.cell.2013.02.023","article-title":"Criteria for Inference of Chromothripsis in Cancer Genomes","volume":"152","author":"Korbel","year":"2013","journal-title":"Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/modpathol.2017.185","article-title":"Genome Profiling Is an Efficient Tool to Avoid the STUMP Classification of Uterine Smooth Muscle Lesions: A Comprehensive Array-Genomic Hybridization Analysis of 77 Tumors","volume":"31","author":"Croce","year":"2018","journal-title":"Mod. Pathol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6891","DOI":"10.1158\/0008-5472.CAN-17-1744","article-title":"Subtype-Specific Tumor-Associated Fibroblasts Contribute to the Pathogenesis of Uterine Leiomyoma","volume":"77","author":"Wu","year":"2017","journal-title":"Cancer Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"207.e1","DOI":"10.1016\/j.ajog.2022.09.024","article-title":"MED12 Mutations in Uterine Leiomyomas: Prediction of Volume Reduction by Gonadotropin-Releasing Hormone Agonists","volume":"228","author":"Nagai","year":"2022","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1002\/gcc.20627","article-title":"Overexpression of HMGA2 in Uterine Leiomyomas Points to Its General Role for the Pathogenesis of the Disease","volume":"48","author":"Klemke","year":"2009","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"34762","DOI":"10.18632\/oncotarget.16711","article-title":"Factors Affecting the Loss of MED12-Mutated Leiomyoma Cells during in Vitro Growth","volume":"8","author":"Bloch","year":"2017","journal-title":"Oncotarget"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"317","DOI":"10.17816\/ecogen606642","article-title":"Differential Effect of Estrogen and Progesterone on in Vitro Growth of Uterine Leiomyoma Cells with Chromosome 7 Deletions","volume":"21","author":"Koltsova","year":"2024","journal-title":"Ecol. Genet."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5448","DOI":"10.1038\/s41467-021-25806-x","article-title":"Parity Associates with Chromosomal Damage in Uterine Leiomyomas","volume":"12","author":"Kuisma","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Liehr, T. (2017). Immunofluorescence Staining for Cytosine Modifications Like 5-Methylcytosine and Its Oxidative Derivatives and FISH. Fluorescence In Situ Hybridization (FISH), Springer. Springer Protocols Handbooks.","DOI":"10.1007\/978-3-662-52959-1"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1007\/s10517-017-3870-3","article-title":"Differential DNA Hydroxymethylation in Human Uterine Leiomyoma Cells Depending on the Phase of Menstrual Cycle and Presence of MED12 Gene Mutations","volume":"163","author":"Pendina","year":"2017","journal-title":"Bull. Exp. Biol. Med."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/1\/96\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:00:22Z","timestamp":1760115622000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/1\/96"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,26]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["ijms26010096"],"URL":"https:\/\/doi.org\/10.3390\/ijms26010096","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2024,12,26]]}}}