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Its persistence on food processing surfaces via biofilm formation presents a major challenge, as conventional sanitizers and antimicrobials exhibit limited efficacy against biofilm-embedded cells. This study investigates a novel approach combining an engineered polysaccharide-degrading enzyme (CAase) with a bacteriocin (thermophilin 110) produced by Streptococcus thermophilus. Laboratory assays evaluated the effectiveness of this combination in disrupting biofilms and inactivating L. monocytogenes on various surfaces. The results demonstrated that CAase effectively disrupts biofilm structures, while thermophilin 110 significantly reduces bacterial growth and viability. 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J. Food Microbiol."},{"key":"ref_3","first-page":"110953","article-title":"Source attribution of Listeria monocytogenes in the Netherlands","volume":"427","author":"Paganini","year":"2024","journal-title":"Int. J. Food Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13073-024-01389-2","article-title":"High density genomic surveillance and risk profiling of clinical Listeria monocytogenes subtypes in Germany","volume":"16","author":"Halbedel","year":"2024","journal-title":"Genome Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"316","DOI":"10.3390\/dairy4020022","article-title":"Listeria monocytogenes: An Inconvenient Hurdle for the Dairy Industry","volume":"4","author":"Ribeiro","year":"2023","journal-title":"Dairy"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"107540","DOI":"10.1016\/j.foodcont.2020.107540","article-title":"Listeria monocytogenes dissemination in farming and primary production: Sources, shedding and control measures","volume":"120","author":"Rodriguez","year":"2020","journal-title":"Food Control"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Townsend, A., Strawn, L.K., Chapman, B.J., and Dunn, L.L. (2021). A Systematic Review of Listeria Species and Listeria monocytogenes Prevalence, Persistence, and Diversity throughout the Fresh Produce Supply Chain. Foods, 10.","DOI":"10.3390\/foods10061427"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3389\/fsufs.2019.00033","article-title":"Listeria Occurrence and Potential Control Strategies in Alternative and Conventional Poultry Processing and Retail","volume":"3","author":"Rothrock","year":"2019","journal-title":"Front. Sustain. Food Syst."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2792","DOI":"10.3168\/jds.2009-2717","article-title":"Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes","volume":"93","author":"Latorre","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.4315\/0362-028X.JFP-14-300","article-title":"Controlling Attachment and Growth of Listeria monocytogenes in Polyvinyl Chloride Model Floor Drains Using a Peroxide Chemical, Chitosan-Arginine, or Heat","volume":"77","author":"Berrang","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.ijfoodmicro.2010.06.021","article-title":"Survival of Listeria monocytogenes on a conveyor belt material with or without antimicrobial additives","volume":"142","author":"Chaitiemwong","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mazaheri, T., Cervantes-Huam\u00e1n, B.R.H., Berm\u00fadez-Capdevila, M., Ripolles-Avila, C., and Rodr\u00edguez-Jerez, J.J. (2021). Listeria monocytogenes Biofilms in the Food Industry: Is the Current Hygiene Program Sufficient to Combat the Persistence of the Pathogen?. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010181"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1574-6968.2002.tb11155.x","article-title":"Comparison of protein patterns of Listeria monocytogenes grown in biofilm or in planktonic mode by proteomic analysis","volume":"210","author":"Namane","year":"2002","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1128\/AEM.03173-14","article-title":"Exploring the Diversity of Listeria monocytogenes Biofilm Architecture by High-Throughput Confocal Laser Scanning Microscopy and the Predominance of the Honeycomb-Like Morphotype","volume":"81","author":"Guilbaud","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4491","DOI":"10.1128\/AEM.00255-08","article-title":"Listeria monocytogenes EGD-e Biofilms: No Mushrooms but a Network of Knitted Chains","volume":"74","author":"Rieu","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7336","DOI":"10.1128\/AEM.69.12.7336-7342.2003","article-title":"Variation in Biofilm Formation among Strains of Listeria monocytogenes","volume":"69","author":"Borucki","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"fnv229","DOI":"10.1093\/femsle\/fnv229","article-title":"Teichoic acid is the major polysaccharide present in the Listeria monocytogenes biofilm matrix","volume":"363","author":"Brauge","year":"2016","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Alonso, A.N., Perry, K.J., Regeimbal, J.M., Regan, P.M., and Higgins, D.E. (2014). Identification of Listeria monocytogenes Determinants Required for Biofilm Formation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0113696"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1111\/mmi.12966","article-title":"Listeria monocytogenes exopolysaccharide: Origin, structure, biosynthetic machinery and c-di-GMP-dependent regulation","volume":"96","author":"Heiss","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kawacka, I., and Olejnik-Schmidt, A. (2024). Gene emrC Associated with Resistance to Quaternary Ammonium Compounds Is Common among Listeria monocytogenes from Meat Products and Meat Processing Plants in Poland. Antibiotics, 13.","DOI":"10.3390\/antibiotics13080749"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Grigore-Gurgu, L., Bucur, F.I., Mihalache, O.A., and Nicolau, A.I. (2024). Comprehensive Review on the Biocontrol of Listeria monocytogenes in Food Products. Foods, 13.","DOI":"10.3390\/foods13050734"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"792162","DOI":"10.3389\/fmicb.2021.792162","article-title":"Different Shades of Listeria monocytogenes: Strain, Serotype, and Lineage-Based Variability in Virulence and Stress Tolerance Profiles","volume":"12","author":"Muchaamba","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ijfoodmicro.2018.03.026","article-title":"Listeria monocytogenes strains show large variations in competitive growth in mixed culture biofilms and suspensions with bacteria from food processing environments","volume":"275","author":"Heir","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1038\/s41598-024-52394-9","article-title":"Elimination of detached Listeria monocytogenes from the biofilm on stainless steel surfaces during milk and cheese processing using natural plant extracts","volume":"14","author":"Ahmed","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Fagerlund, A., Heir, E., M\u00f8retr\u00f8, T., and Langsrud, S. (2020). Listeria Monocytogenes Biofilm Removal Using Different Commercial Cleaning Agents. Molecules, 25.","DOI":"10.3390\/molecules25040792"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/S0023-6438(03)00112-9","article-title":"Efficacy of plant essential oils as antimicrobial agents against Listeria monocytogenes in hotdogs","volume":"36","author":"Singh","year":"2003","journal-title":"LWT"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e0116723","DOI":"10.1128\/spectrum.01167-23","article-title":"Context matters: Environmental microbiota from ice cream processing facilities affected the inhibitory performance of two lactic acid bacteria strains against Listeria monocytogenes","volume":"12","author":"Rolon","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0026521","DOI":"10.1128\/AEM.00265-21","article-title":"Disrupting Irreversible Bacterial Adhesion and Biofilm Formation with an Engineered Enzyme","volume":"87","author":"Mayton","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"109445","DOI":"10.1016\/j.foodcont.2022.109445","article-title":"Enhancing detection of Listeria monocytogenes in food products using an enzyme","volume":"145","author":"Felton","year":"2022","journal-title":"Food Control"},{"key":"ref_30","first-page":"241","article-title":"Antibacterial activities of bacteriocins: Application in foods and pharmaceuticals","volume":"5","author":"Yang","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1080\/10408399409527650","article-title":"Nisin as a model food preservative","volume":"34","author":"Hansen","year":"1994","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"709959","DOI":"10.3389\/fmicb.2021.709959","article-title":"Antibacterial Activity of Pediocin and Pediocin-Producing Bacteria Against Listeria monocytogenes in Meat Products","volume":"12","author":"Khorshidian","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Heir, E., Jensen, M.R., Aasli, A.W., Berget, I., and Holck, A.L. (2023). Reduction and Growth Inhibition of Listeria monocytogenes by Use of Anti-Listerial Nisin, P100 Phages and Buffered Dry Vinegar Fermentates in Standard and Sodium-Reduced Cold-Smoked Salmon. Foods, 12.","DOI":"10.3390\/foods12244391"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Santativongchai, P., Tulayakul, P., and Jeon, B. (2023). Enhancement of the Antibiofilm Activity of Nisin against Listeria monocytogenes Using Food Plant Extracts. Pathogens, 12.","DOI":"10.3390\/pathogens12030444"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1007\/s10529-023-03419-2","article-title":"Antimicrobial activity of thermophilin 110 against the opportunistic pathogen Cutibacterium acnes","volume":"45","author":"Renye","year":"2023","journal-title":"Biotechnol. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Cui, Y., Xu, T., Qu, X., Hu, T., Jiang, X., and Zhao, C. (2016). New Insights into Various Production Characteristics of Streptococcus thermophilus Strains. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17101701"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7195","DOI":"10.1128\/JB.00966-07","article-title":"Quorum-Sensing Regulation of the Production of Blp Bacteriocins in Streptococcus thermophilus","volume":"189","author":"Fontaine","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s00284-005-4540-7","article-title":"Thermophilin 110: A Bacteriocin of Streptococcus thermophilus ST110","volume":"51","author":"Gilbreth","year":"2005","journal-title":"Curr. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"107941","DOI":"10.1016\/j.foodcont.2021.107941","article-title":"Anti-listerial activity of thermophilin 110 and pediocin in fermented milk and whey","volume":"125","author":"Ceruso","year":"2021","journal-title":"Food Control"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e00647","DOI":"10.1016\/j.btre.2021.e00647","article-title":"Thermophilin 110 inhibits growth and biofilm formation of Streptococcus mutans","volume":"31","author":"Renye","year":"2021","journal-title":"Biotechnol. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5408","DOI":"10.1007\/s13197-014-1666-2","article-title":"Strategies for the use of bacteriocins in Gram-negative bacteria: Relevance in food microbiology","volume":"52","author":"Vanetti","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4519","DOI":"10.1128\/AEM.69.8.4519-4526.2003","article-title":"Biocontrol of Listeria monocytogenes on Fresh-Cut Produce by Treatment with Lytic Bacteriophages and a Bacteriocin","volume":"69","author":"Leverentz","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"594","DOI":"10.3389\/fmicb.2018.00594","article-title":"Application of Bacteriocins and Protective Cultures in Dairy Food Preservation","volume":"9","author":"Silva","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1128\/AEM.00070-15","article-title":"Efficacies of Nisin A and Nisin V Semipurified Preparations Alone and in Combination with Plant Essential Oils for Controlling Listeria monocytogenes","volume":"81","author":"Field","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1093\/jac\/dkg229","article-title":"The bacteriocin piscicolin 126 retains antilisterial activity in vivo","volume":"51","author":"Ingham","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ijfoodmicro.2009.05.005","article-title":"In vivo study on the effectiveness of pediocin PA-1 and Pediococcus acidilactici UL5 at inhibiting Listeria monocytogenes","volume":"133","author":"Dabour","year":"2009","journal-title":"Int. J. Food Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.ijbiomac.2020.07.192","article-title":"Characterization and antibacterial action mode of bacteriocin BMP32r and its application as antimicrobial agent for the therapy of multidrug-resistant bacterial infection","volume":"164","author":"Qiao","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.4315\/0362-028X-63.9.1204","article-title":"Persistent Listeria monocytogenes Strains Show Enhanced Adherence to Food Contact Surface after Short Contact Times","volume":"63","author":"Miettinen","year":"2000","journal-title":"J. Food Prot."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.4315\/0362-028X.JFP-17-466","article-title":"A Comparative Study of the Susceptibility of Listeria Species to Sanitizer Treatments When Grown under Planktonic and Biofilm Conditions","volume":"81","author":"Fox","year":"2018","journal-title":"J. Food Prot."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7711","DOI":"10.1128\/AEM.01065-06","article-title":"Resistance of Listeria monocytogenes Biofilms to Sanitizing Agents in a Simulated Food Processing Environment","volume":"72","author":"Pan","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ) (2016). Evaluation of the safety and efficacy of ListexTM P100 for reduction of pathogens on different ready-to-eat (RTE) food products. EFSA J., 14, e04565.","DOI":"10.2903\/j.efsa.2016.4565"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104421","DOI":"10.1016\/j.fm.2023.104421","article-title":"Efficacy of cleaning and sanitizing procedures to reduce Listeria monocytogenes on food contact surfaces commonly found in fresh produce operations","volume":"118","author":"Ohman","year":"2023","journal-title":"Food Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Colagiorgi, A., Di Ciccio, P., Zanardi, E., Ghidini, S., and Ianieri, A. (2016). A Look inside the Listeria monocytogenes Biofilms Extracellular Matrix. 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