{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:34:52Z","timestamp":1759970092278,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China National Funds for Young Scientists","award":["82405008","CXPY202106","GJHZ202309"],"award-info":[{"award-number":["82405008","CXPY202106","GJHZ202309"]}]},{"name":"Shihezi University Youth Innovation Talent Cultivation Project","award":["82405008","CXPY202106","GJHZ202309"],"award-info":[{"award-number":["82405008","CXPY202106","GJHZ202309"]}]},{"name":"International Science and Technology Cooperation Promotion Project of Shihezi University","award":["82405008","CXPY202106","GJHZ202309"],"award-info":[{"award-number":["82405008","CXPY202106","GJHZ202309"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Euphorbia uralensis belongs to the family Euphorbiaceae and is widely distributed in northern Xinjiang, making it a characteristic plant of the region in Xinjiang, China. The chemical composition and biological activity of Euphorbia uralensis have not yet been reported, although certain compounds isolated from Euphorbia plants in Xinjiang, China, have demonstrated exceptional multidrug resistance (MDR) reversal. This study aims to investigate the chemical components present in Euphorbia uralensis with the potential to reverse MDR. The aerial parts of Euphorbia uralensis were extracted using organic solvents of varying polarities, resulting in dichloromethane (Fr-E) and petroleum ether (Fr-S) fractions, which exhibited greater MDR reversal activity than the other fractions. The chemical constituents of the Fr-S fraction were analyzed using GC-MS. The chemical components of the Fr-E fraction were isolated and purified using column chromatography. The most effective compounds with MDR reversal activity were screened out, and the mechanism was investigated using molecular docking, molecular dynamics simulations, Western blotting, and rhodamine 123 staining. GC-MS analysis showed that the Fr-S fraction was rich in triterpenes, fatty acids, phenols, and long-chain alkanes, all of which were identified for the first time in Euphorbia uralensis. Among these, palmitic acid was present at a content level of 15.86%. This study notably unveils the discovery of a new compound and 16 previously recorded compounds for the first time in this plant, with the main types identified as steroids, sesquiterpenes, and flavonoids. The isolated compounds were tested for cytotoxicity and MDR reversal activity. The new compounds Euphouralosides A, pubinernoid A, naringenin, and punigratine showed good MDR reversal activity against MCF-7 and MCF-7\/ADR cell lines. Punigratine was the most active compound. Moreover, punigratine could stably bind to the ABCB1 protein. Western blot analysis revealed that punigratine did not affect the expression of the ABCB1 protein in cells (p &gt; 0.05). However, following treatment with punigratine (0.16 \u03bcM), there was a significant increase the intracellular accumulation of Rh123 in MCF-7\/ADR cells (p &lt; 0.05). These findings suggest that punigratine can inhibit the efflux of the ABCB1 protein, thereby overcoming MDR in tumors. This study provides a foundation for further research on the biological activity and medicinal potential of Euphorbia uralensis.<\/jats:p>","DOI":"10.3390\/ijms26010412","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T09:17:02Z","timestamp":1736155022000},"page":"412","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Study on the Chemical Composition and Multidrug Resistance Reversal Activity of Euphorbia uralensis (Euphorbiaceae)"],"prefix":"10.3390","volume":"26","author":[{"given":"Yina","family":"Ding","sequence":"first","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhao","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianru","family":"Dang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zubair","family":"Akram","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anam","family":"Arshad","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haochan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chimengul","family":"Turghun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Xinjiang Phytomedicine Resource and Uilization, Ministry of Education, Shihezi 832002, China"},{"name":"School of Pharmacy, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s00268-022-06883-5","article-title":"Time to treatment initiation for six cancer types: An analysis of data from a nationwide registry in japan","volume":"47","author":"Watanabe","year":"2023","journal-title":"World J. Surg."},{"key":"ref_2","first-page":"163","article-title":"CALCRL induces resistance to daunorubicin in acute myeloid leukemia cells through upregulation of XRCC5\/TYK2\/JAK1 pathway","volume":"35","author":"Tang","year":"2024","journal-title":"Anti-Cancer Drugs"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"10690","DOI":"10.1038\/ncomms10690","article-title":"Diverse drug-resistance mechanisms can emerge from drug-tolerant cancer persister cells","volume":"7","author":"Ramirez","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7059","DOI":"10.2174\/0929867325666180410110729","article-title":"Diverse Targeted Approaches to Battle Multidrug Resistance in Cancer","volume":"26","author":"Shankaraiah","year":"2019","journal-title":"Curr. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1038\/nrc3599","article-title":"Cancer drug resistance: An evolving paradigm","volume":"13","author":"Holohan","year":"2013","journal-title":"Nat. Rev. Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1021\/acs.jnatprod.5b01055","article-title":"Natural products as sources of new drugs from 1981 to 2014","volume":"79","author":"Newman","year":"2016","journal-title":"J. Nat. Prod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.2174\/1871520617666171016105704","article-title":"Research Progress in Reversal of Tumor Multi-drug Resistance via Natural Products","volume":"17","author":"Guo","year":"2017","journal-title":"Anti-Cancer Agents Med. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zou, J.Y., Chen, Q.L., Luo, X.C., Damdinjav, D., Abdelmohsen, U.R., Li, H.Y., Battulga, T., Chen, H.B., Wang, Y.Q., and Zhang, J.Y. (2023). Natural Products Reverse Cancer Multi drug Resistance. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1348076"},{"key":"ref_9","first-page":"471","article-title":"Traditional Chinese medicine reverses cancer multidrug resistance and its mechanism","volume":"24","author":"Wei","year":"2022","journal-title":"Clin. Transl. Oncol. Off. Publ. Fed. Span. Oncol. Soc. Natl. Cancer Inst. Mex."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1080\/13880209.2020.1755318","article-title":"Comparison of the short-chain fatty acids in normal rat faeces after the treatment of Euphorbia kansui, a traditional Chinese medicine for edoema","volume":"58","author":"Jiang","year":"2020","journal-title":"Pharm. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhu, H., Ren, X.X., Huang, Y.B., Su, T., and Yang, L. (2023). Chemical Constituents of Euphorbia stracheyi Boiss (Euphorbiaceae). Metabolites, 13.","DOI":"10.3390\/metabo13070852"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1080\/14786419.2016.1277345","article-title":"Isolation and characterisation of cytotoxic compounds from Euphorbia clementei Boiss","volume":"31","author":"Benmerache","year":"2017","journal-title":"Nat. Prod. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1055\/a-1479-2866","article-title":"Diterpenes from Euphorbia myrsinites and their anti-inflammatory property","volume":"87","author":"Grauso","year":"2021","journal-title":"Planta Medica"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Magozwi, D.K., Dinala, M., Mokwana, N., Siwe-Noundou, X., Krause, R.W.M., Sonopo, M., McGaw, L.J., Augustyn, W.A., and Tembu, V.J. (2021). Flavonoids from the Genus Euphorbia: Isolation, Structure, Pharmacological Activities and Structure\u2013Activity Relationships. Pharmaceuticals, 4.","DOI":"10.3390\/ph14050428"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.phytochem.2007.01.017","article-title":"Diterpenoids and triterpenoids from Euphorbia guyoniana","volume":"68","author":"Hamada","year":"2007","journal-title":"Phytochemistry"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rojas-Jim\u00e9nez, S., Valladares-Cisneros, M.G., Salinas-S\u00e1nchez, D.O., P\u00e9rez-Ramos, J., S\u00e1nchez-P\u00e9rez, L., P\u00e9rez-Guti\u00e9rrez, S., and Campos-Xolalpa, N. (2024). Anti-Inflammatory and Cytotoxic Compounds Isolated from Plants of Euphorbia Genus. Molecules, 29.","DOI":"10.3390\/molecules29051083"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1021\/acs.jnatprod.6b01099","article-title":"Terpenoids from Euphorbia soongarica and Their Multidrug Resi stance Reversal Activity","volume":"80","author":"Gao","year":"2017","journal-title":"J. Nat. Prod."},{"key":"ref_18","first-page":"2222","article-title":"Stellera chamaejasme against multidrug resistance of triple-negative breast cancer MDA-MB-231 cell through Nrf2","volume":"49","author":"Zeng","year":"2024","journal-title":"China J. Chin. Mater. Medica"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1134\/S1068162021050204","article-title":"Isolation of Bioactive Compounds from Pistacia integerrima with Promising Effects on Reverse Cancer Multidrug Resistance","volume":"47","author":"Bawazeer","year":"2021","journal-title":"Russ. J. Bioorganic Chem."},{"key":"ref_20","first-page":"315","article-title":"Chemical constituents of the aerial parts of Euphorbia sororia","volume":"38","author":"Zhang","year":"2007","journal-title":"J. China Pharm. Univ."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hasan, A., Tang, D., Nijat, D., Yang, H.Q., and Aisa, H.A. (2021). Diterpenoids from Euphorbia glomerulans with potential reversal activities against P-glycoprotein-mediated multidrug resistance. Bioorganic Chem., 117.","DOI":"10.1016\/j.bioorg.2021.105442"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.phytochem.2015.12.003","article-title":"Diterpenoid constituents of Euphorbia macrorrhiza","volume":"122","author":"Gao","year":"2016","journal-title":"Phytochemistry"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Yang, H.Q., Mamatjan, A., Tang, D., and Aisa, H.A. (2021). Jatrophane diterpenoids as multidrug resistance modulators from Euphorbia sororia. Bioorganic Chem., 112.","DOI":"10.1016\/j.bioorg.2021.104989"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1002\/hlca.200690114","article-title":"Three Novel Terpenoids from Schisandra pubescens var. Pubinervis","volume":"89","author":"Huang","year":"2006","journal-title":"Helv. Chim. Acta"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1016\/j.phytochem.2005.09.012","article-title":"Three sesquiterpene hydrocarbons from the roots of Panax ginseng C. A. Meyer (Araliaceae)","volume":"66","author":"Richter","year":"2005","journal-title":"Phytochemistry"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"259","DOI":"10.4314\/tjpr.v11i2.12","article-title":"Isolation and characterization of lup-20 (29)-ene-3, 28-diol (Betulin) from the stem-bark of Adenium obesum (Apocynaceae)","volume":"11","author":"Tijjani","year":"2012","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_27","first-page":"1175","article-title":"Chemical constituents from branch of Macaranga adenantha and their TNF-\u03b1 inhibitory activity","volume":"32","author":"Ma","year":"2007","journal-title":"China J. Chin. Mater. Medica"},{"key":"ref_28","first-page":"60","article-title":"Chemical constituents from leaves of Zanthoxylum bungeanum Maxim","volume":"38","author":"Jiao","year":"2022","journal-title":"J. Qiqihar Univ. (Nat. Sci. Ed.)"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.3109\/13880200903486644","article-title":"Antimicrobial and antioxidant effects of phenolic constituents from Klainedoxa gabonensis","volume":"48","author":"Wansi","year":"2010","journal-title":"Pharm. Biol."},{"key":"ref_30","first-page":"1775","article-title":"Study on the chemical constituents of Elephantopus mollis","volume":"35","author":"Liang","year":"2012","journal-title":"J. Chin. Med. Mater."},{"key":"ref_31","first-page":"700","article-title":"A new bibenzyl derivative from stems of Dendrobium officinale","volume":"48","author":"Meng","year":"2023","journal-title":"Chin. Med. Mag. China"},{"key":"ref_32","first-page":"2718","article-title":"Chemical constituents from ethyl acetate fraction of Callicarpa giraldii and their anti-inflammatory activities","volume":"43","author":"Xu","year":"2021","journal-title":"Chin. Tradit. Pat. Med."},{"key":"ref_33","first-page":"181","article-title":"Chemical Constituents of Asparagus officinalis","volume":"4","author":"Huang","year":"2006","journal-title":"Chin. J. Nat. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1002\/cjoc.200790155","article-title":"New Aliphatic Alcohol and Ester Constituents from Rice Hulls of Oryza sativa","volume":"25","author":"Chung","year":"2007","journal-title":"Chin. J. Chem."},{"key":"ref_35","first-page":"383","article-title":"Study on the Chemical Constituents of the Tropical Seagrass Enhalus acoroides","volume":"31","author":"Wang","year":"2018","journal-title":"J. Hainan Norm. Univ. (Nat. Sci.)"},{"key":"ref_36","first-page":"827","article-title":"Chemical constituents in roots of Polygala fallax and their anti-oxidation activities in vitro","volume":"30","author":"Lin","year":"2005","journal-title":"China J. Chin. Mater. Medica"},{"key":"ref_37","first-page":"5218","article-title":"Study on chemical constituents from active ethyl acetate fraction of Dendrobium officinale","volume":"52","author":"Zhou","year":"2021","journal-title":"Chin. Tradit. Herb. Drugs"},{"key":"ref_38","first-page":"668","article-title":"Study on the Chemical Constituents of Roots from Euphorbia ebracteolata Hayata","volume":"58","author":"Zhang","year":"2023","journal-title":"Chin. Pharm. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1080\/14786419.2016.1146883","article-title":"Punigratane, a novel pyrrolidine alkaloid from Punica granatum rind with putative efflux inhibition activity","volume":"30","author":"Rafiq","year":"2016","journal-title":"Nat. Prod. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1007\/s10600-018-2510-0","article-title":"Phytochemical Constituents Isolated from Euphorbia rapulum","volume":"54","author":"Liu","year":"2018","journal-title":"Chem. Nat. Compd."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1021\/acs.jnatprod.8b00507","article-title":"Jatrophane Diterpenoids from Euphorbia glomerulans","volume":"82","author":"Hasan","year":"2019","journal-title":"J. Nat. Prod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"113444","DOI":"10.1016\/j.phytochem.2022.113444","article-title":"Jatrophane diterpenoids from Euphorbia microcarpa (prokh.) krylov with multidrug resistance modulating activity","volume":"204","author":"Rouzimaimaiti","year":"2022","journal-title":"Phytochemistry"},{"key":"ref_43","first-page":"1001","article-title":"Reversal effect of 4 alkaloids on apoptosis of being obtained multi-drug resistance to tumour cell","volume":"28","author":"Li","year":"2006","journal-title":"Chin. Tradit. Pat. Med."},{"key":"ref_44","first-page":"79","article-title":"Action Mechanism Progress of Chinese Medicinal and Its Active Ingredients in Treatment of Hepatic Carcinoma","volume":"39","author":"Qiu","year":"2022","journal-title":"Inf. Tradit. Chin. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1002\/mrc.5070","article-title":"Plant-like cadinane sesquiterpenes from an actinobacterial mangrove endophyte","volume":"59","author":"Ding","year":"2021","journal-title":"Magn. Reson. Chem. MRC"},{"key":"ref_46","first-page":"451","article-title":"miR-1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of in tracellular drugs and apoptosis of cells","volume":"55","author":"Deng","year":"2019","journal-title":"Int. J. Oncol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3892\/ijo.2017.4005","article-title":"Uncaria alkaloids reverse ABCB1-mediated cancer multidrug resistance","volume":"51","author":"Huang","year":"2017","journal-title":"Int. J. Oncol."},{"key":"ref_48","first-page":"89","article-title":"Effect of temperature on the stability of lactoglobulin: A molecular dynamics simulation","volume":"46","author":"Liu","year":"2020","journal-title":"Food Ferment. Ind."},{"key":"ref_49","unstructured":"Sun, W., and Jiao, K. (2001). Interactions of Small Molecules with Protein and Its Application to Determination of Proteins. J. Qingdao Inst. Chem. Technol., 299\u2013301."},{"key":"ref_50","first-page":"2667","article-title":"Identification of immune-related biomarkers of Alzheimer\u2019s disease and prediction of medicine-food homology traditional Chinese medicines based on bioinformatics analysis and dynamic simulation","volume":"55","author":"Liang","year":"2024","journal-title":"Chin. Tradit. Herb. Drugs"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kumju, Y., and Mira, J. (2024). Determination of Potential Lead Compound from Magnolia officinalis for Alzheimer\u2019s Disease through Pharmacokinetic Prediction, Molecular Docking, Dynamic Simulation, and Experimental Validation. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms251910507"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"105390","DOI":"10.1016\/j.arabjc.2023.105390","article-title":"Molecular mechanism of Yi-Qi-Yang-Yin-Ye against obesity in rats using network pharmacology, molecular docking, and molecular dynamics simulations","volume":"17","author":"Sun","year":"2024","journal-title":"Arab. J. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1016\/j.bbagen.2016.04.007","article-title":"Rhamnetin induces sensitization of hepatocellular carcinoma cells to a small molecular kinase inhibitor or chemotherapeutic agents","volume":"1860","author":"Jia","year":"2016","journal-title":"BBA-Gen. Subjects. Gen. Subj."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1002\/jcb.26391","article-title":"Kaempferol increases apoptosi s in human acute promyelocytic leukemia cells and inhibits multidrug resistance genes","volume":"119","author":"Moradzadeh","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"106303","DOI":"10.1016\/j.ejps.2022.106303","article-title":"Kaempferol suppression of acute colitis is regulated by the efflux transporters BCRP and MRP2","volume":"179","author":"Liu","year":"2022","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1248\/bpb.28.83","article-title":"Induction of Apoptosis in Human Promyelocytic Leukemia Cell Line HL-60 by C-Benzylated Dihydrochalcones, Uvaretin, Isouvaretin and Diuvaretin (Medicinal Chemistry)","volume":"28","author":"Noriyoshi","year":"2005","journal-title":"Biol. Pharm. Bull."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Piehl, D.W., and Burley, S.K. (2024). Parallel delivery of experimentally determined structures and computed structure models at RCSB protein data bank (RCSB PDB, RCSB. ORG). Biophys. J., 123.","DOI":"10.1016\/j.bpj.2023.11.1746"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/1\/412\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:23:23Z","timestamp":1759919003000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/1\/412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":57,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["ijms26010412"],"URL":"https:\/\/doi.org\/10.3390\/ijms26010412","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2025,1,6]]}}}