{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:51:01Z","timestamp":1788371461070,"version":"build-2803163510"},"reference-count":59,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32272965"],"award-info":[{"award-number":["32272965"]}]},{"name":"National Natural Science Foundation of China","award":["32202753"],"award-info":[{"award-number":["32202753"]}]},{"name":"National Natural Science Foundation of China","award":["31972639"],"award-info":[{"award-number":["31972639"]}]},{"name":"National Natural Science Foundation of China","award":["2022-BS-170"],"award-info":[{"award-number":["2022-BS-170"]}]},{"name":"National Natural Science Foundation of China","award":["2023-MSLH-281"],"award-info":[{"award-number":["2023-MSLH-281"]}]},{"name":"Science and Technology Department of Liaoning Province","award":["32272965"],"award-info":[{"award-number":["32272965"]}]},{"name":"Science and Technology Department of Liaoning Province","award":["32202753"],"award-info":[{"award-number":["32202753"]}]},{"name":"Science and Technology Department of Liaoning Province","award":["31972639"],"award-info":[{"award-number":["31972639"]}]},{"name":"Science and Technology Department of Liaoning Province","award":["2022-BS-170"],"award-info":[{"award-number":["2022-BS-170"]}]},{"name":"Science and Technology Department of Liaoning Province","award":["2023-MSLH-281"],"award-info":[{"award-number":["2023-MSLH-281"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fatty liver hemorrhagic syndrome (FLHS) in laying hens is a nutritional and metabolic disease involving liver enlargement, hepatic steatosis, and hepatic hemorrhage as the primary symptoms. The syndrome is prone to occur during the peak laying period of laying hens, which has resulted in significant economic losses in the laying hen breeding industry; however, the specific pathogenesis of FLHS remains unclear. Our group and previous studies have shown that bile acid levels are significantly decreased during the development of fatty liver and that targeted activation of bile acid\u2013related signaling pathways is beneficial for preventing and treating fatty liver. In this study, we generated a FLHS laying hen model by feeding hens a high-energy, low-protein diet, with goose deoxycholic acid (CDCA) given as an intervention. HE staining, fluorescence quantitative PCR, and ELISA were used to evaluate the effects of CDCA on pathological changes and inflammatory responses in the liver. The results showed that hepatic hemorrhage in FLHS laying hens was reduced after CDCA treatment. Furthermore, fat vacuoles and transaminase levels decreased significantly. In addition, expression levels of M1-type macrophage markers and polarization products were significantly reduced, and the expression of pro-inflammatory regulatory factors related to the JAK-STAT signaling pathway, LPS-TLR4-Myd88\u2013NF-kB signaling pathway, and NLRP3 inflammasomes decreased significantly as well. Expression levels of M2-type macrophage markers and polarization products increased significantly, as did the expression of anti-inflammatory regulators related to the JAK-STAT signaling pathway. These results suggest that CDCA ameliorates liver injury in laying hens with FLHS by inhibiting macrophage M1-type polarization and the resulting pro-inflammatory response, thereby promoting M2-type macrophage polarization and an anti-inflammatory response.<\/jats:p>","DOI":"10.3390\/ijms26010429","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T10:40:52Z","timestamp":1736160052000},"page":"429","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Goose Deoxycholic Acid Ameliorates Liver Injury in Laying Hens with Fatty Liver Hemorrhage Syndrome by Inhibiting the Inflammatory Response"],"prefix":"10.3390","volume":"26","author":[{"given":"Nannan","family":"Wang","sequence":"first","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Li","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyi","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengqi","family":"Li","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4509-0893","authenticated-orcid":false,"given":"Jiancheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaofeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.3382\/ps.0511707","article-title":"Study on fatty liver-hemorrhagic syndrome (FLHS)","volume":"51","author":"Wolford","year":"1972","journal-title":"Poult. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/S0016-5085(98)70599-2","article-title":"Steatohepatitis: A tale of two \u201chits\u201d","volume":"114","author":"Day","year":"1998","journal-title":"Gastroenterology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"113432","DOI":"10.1016\/j.intimp.2024.113432","article-title":"Preventive effects of matrine on LPS-induced inflammation in RAW 264.7 cells and intestinal damage in mice through the TLR4\/NF-\u03baB\/MAPK pathway","volume":"143","author":"Mao","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1080\/08923973.2024.2390455","article-title":"Sunitinib alleviates hepatic ischemia reperfusion injury by inhibiting the JAK2\/STAT pathway and promoting the M2 polarization of macrophages","volume":"46","author":"Li","year":"2024","journal-title":"Immunopharmacol. Immunotoxicol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1080\/13543784.2017.1385760","article-title":"Beneficial effects of bile acid receptor agonists in pulmonary disease models","volume":"26","author":"Comeglio","year":"2017","journal-title":"Expert Opin. Investig. Drugs"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Oleszycka, E., O\u2019Brien, E.C., Freeley, M., Lavelle, E.C., and Long, A. (2023). Bile acids induce IL-1\u03b1 and drive NLRP3 inflammasome-independent production of IL-1\u03b2 in murine dendritic cells. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1285357"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1055\/s-0038-1669940","article-title":"Role of TGR5 (GPBAR1) in Liver Disease","volume":"38","author":"Keitel","year":"2018","journal-title":"Semin. Liver Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.trsl.2016.12.001","article-title":"Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice","volume":"182","author":"Ji","year":"2017","journal-title":"Transl. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.psj.2020.11.036","article-title":"Protective effect of the new prepared Atractylodes macrocephala Koidz polysaccharide on fatty liver hemorrhagic syndrome in laying hens","volume":"100","author":"Miao","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wei, F., Yang, X., Zhang, M., Xu, C., Hu, Y., and Liu, D. (2022). Akkermansia muciniphila Enhances Egg Quality and the Lipid Profile of Egg Yolk by Improving Lipid Metabolism. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.927245"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1080\/03079457.2018.1538550","article-title":"Fatty liver hemorrhagic syndrome occurrence in laying hens: Impact of production system","volume":"48","author":"Shini","year":"2019","journal-title":"Avian Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"101320","DOI":"10.1016\/j.psj.2021.101320","article-title":"Untargeted and targeted metabolomics profiling reveals the underlying pathogenesis and abnormal arachidonic acid metabolism in laying hens with fatty liver hemorrhagic syndrome","volume":"100","author":"Meng","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"skad045","DOI":"10.1093\/jas\/skad045","article-title":"Alleviation effect of conjugated linoleic acid on estradiol benzoate induced fatty liver hemorrhage syndrome in Hy-line male chickens","volume":"101","author":"Wang","year":"2023","journal-title":"J. Anim. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1007\/s11259-024-10420-1","article-title":"Development of fatty liver disease model using high cholesterol and low choline diet in white leghorn chickens","volume":"48","author":"Yadav","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"103785","DOI":"10.1016\/j.psj.2024.103785","article-title":"Quantitative lipidomics reveals the changes of lipids and antioxidant capacity in egg yolk from laying hens with fatty liver hemorrhagic syndrome","volume":"103","author":"Guo","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Yang, X., Li, D., Zhang, M., Feng, Y., Jin, X., Liu, D., Guo, Y., and Hu, Y. (2023). Ginkgo biloba extract alleviates fatty liver hemorrhagic syndrome in laying hens via reshaping gut microbiota. J. Anim. Sci. Biotechnol., 14.","DOI":"10.1186\/s40104-023-00900-w"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102034","DOI":"10.1016\/j.psj.2022.102034","article-title":"Dietary supplementation of salidroside alleviates liver lipid metabolism disorder and inflammatory response to promote hepatocyte regeneration via PI3K\/AKT\/Gsk3-\u03b2 pathway","volume":"101","author":"Cui","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1055\/a-2277-4805","article-title":"Bioprotective Role of Phytocompounds Against the Pathogenesis of Non-alcoholic Fatty Liver Disease to Non-alcoholic Steatohepatitis: Unravelling Underlying Molecular Mechanisms","volume":"90","author":"Banerjee","year":"2024","journal-title":"Planta Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e2308866","DOI":"10.1002\/advs.202308866","article-title":"Hepatic-Accumulated Obeticholic Acid and Atorvastatin Self-Assembled Nanocrystals Potentiate Ameliorative Effects in Treatment of Metabolic-Associated Fatty Liver Disease","volume":"11","author":"Lu","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"122355","DOI":"10.1016\/j.lfs.2023.122355","article-title":"Obeticholic acid protects against lithocholic acid-induced exogenous cell apoptosis during cholestatic liver injury","volume":"337","author":"Lu","year":"2024","journal-title":"Life Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1016\/j.apsb.2024.02.017","article-title":"A combined nanotherapeutic approach targeting farnesoid X receptor, ferroptosis, and fibrosis for nonalcoholic steatohepatitis treatment","volume":"14","author":"Fu","year":"2024","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"122380","DOI":"10.1016\/j.lfs.2023.122380","article-title":"Bile acids metabolism in the gut-liver axis mediates liver injury during lactation","volume":"338","author":"Huang","year":"2024","journal-title":"Life Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"122182","DOI":"10.1016\/j.lfs.2023.122182","article-title":"The implication of LPS\/TLR4 and FXR receptors in hepatoprotective efficacy of indole-3-acetic acid and chenodeoxycholic acid","volume":"334","author":"Aljarboa","year":"2023","journal-title":"Life Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hsu, C.C., Cheng, K.C., Li, Y., Hsu, P.H., Cheng, J.T., and Niu, H.S. (2021). TGR5 Expression Is Associated with Changes in the Heart and Urinary Bladder of Rats with Metabolic Syndrome. Life, 11.","DOI":"10.3390\/life11070695"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1111\/jre.12912","article-title":"Hyperglycemia modulates M1\/M2 macrophage polarization via reactive oxygen species overproduction in ligature-induced periodontitis","volume":"56","author":"Zhang","year":"2021","journal-title":"J. Periodontal. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"104475","DOI":"10.1016\/j.cellimm.2021.104475","article-title":"CD36 regulates LPS-induced acute lung injury by promoting macrophages M1 polarization","volume":"372","author":"Sun","year":"2022","journal-title":"Cell. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.mce.2015.07.012","article-title":"Chenodeoxycholic acid, an endogenous FXR ligand alters adipokines and reverses insulin resistance","volume":"414","author":"Shihabudeen","year":"2015","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.placenta.2024.05.129","article-title":"Altered placental macrophage numbers and subsets in pregnancies complicated with intrahepatic cholestasis of pregnancy (ICP) compared to healthy pregnancies","volume":"153","author":"Beck","year":"2024","journal-title":"Placenta"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhuang, L., Jia, N., Zhang, L., Zhang, Q., Antwi, S.O., Sartorius, K., Wu, K., Sun, D., Xi, D., and Lu, Y. (2024). Gpbar-1\/cAMP\/PKA signaling mitigates macrophage-mediated acute cholestatic liver injury via antagonizing NLRP3-ASC inflammasome. Biochim. Biophys. Acta Mol. Basis Dis., 1870.","DOI":"10.1016\/j.bbadis.2024.167266"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/nature15541","article-title":"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling","volume":"526","author":"Kayagaki","year":"2015","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zangerolamo, L., Solon, C., Soares, G.M., Engel, D.F., Velloso, L.A., Boschero, A.C., Carneiro, E.M., and Barbosa, H.C.L. (2021). Energy homeostasis deregulation is attenuated by TUDCA treatment in streptozotocin-induced Alzheimer\u2019s disease mice model. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-97624-6"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Attia, Y.M., Tawfiq, R.A., Gibriel, A.A., Ali, A.A., Kassem, D.H., Hammam, O.A., and Elmazar, M.M. (2021). Activation of FXR modulates SOCS3\/Jak2\/STAT3 signaling axis in a NASH-dependent hepatocellular carcinoma animal model. Biochem. Pharmacol., 186.","DOI":"10.1016\/j.bcp.2021.114497"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.bbadis.2009.04.004","article-title":"Reciprocal regulation of the bile acid-activated receptor FXR and the interferon-gamma-STAT-1 pathway in macrophages","volume":"1792","author":"Renga","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Meng, C., Liu, Y., Ming, Y., Lu, C., Li, Y., Zhang, Y., Su, D., Gao, X., and Yuan, Q. (2024). Enhancing Liver Delivery of Gold Nanoclusters via Human Serum Albumin Encapsulation for Autoimmune Hepatitis Alleviation. Pharmaceutics, 16.","DOI":"10.3390\/pharmaceutics16010110"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s00018-020-03656-y","article-title":"TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling","volume":"78","author":"Ciesielska","year":"2021","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/imm.12910","article-title":"Implications of macrophage polarization in autoimmunity","volume":"154","author":"Funes","year":"2018","journal-title":"Immunology"},{"key":"ref_37","first-page":"8253904","article-title":"Acupuncture Attenuates Inflammation in Microglia of Vascular Dementia Rats by Inhibiting miR-93-Mediated TLR4\/MyD88\/NF-\u03baB Signaling Pathway","volume":"2020","author":"Wang","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1172\/JCI126428","article-title":"Ferroptotic cell death and TLR4\/Trif signaling initiate neutrophil recruitment after heart transplantation","volume":"129","author":"Li","year":"2019","journal-title":"J. Clin. Investig."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s12026-021-09188-2","article-title":"MyD88 and beyond: A perspective on MyD88-targeted therapeutic approach for modulation of host immunity","volume":"69","author":"Saikh","year":"2021","journal-title":"Immunol. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Milivojac, T., Grabe\u017e, M., Krivoku\u0107a, A., Mali\u010devi\u0107, U., Gaji\u0107 Boji\u0107, M., \u0110ukanovi\u0107, \u0110., Uletilovi\u0107, S., Mandi\u0107-Kova\u010devi\u0107, N., Cvjetkovi\u0107, T., and Barud\u017eija, M. (Mol. Cell. Biochem., 2024). Ursodeoxycholic and chenodeoxycholic bile acids attenuate systemic and liver inflammation induced by lipopolysaccharide in rats, Mol. Cell. Biochem., Online ahead of print.","DOI":"10.21203\/rs.3.rs-3936668\/v1"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3109\/07357907.2012.762781","article-title":"Bile acids affect the growth of human cholangiocarcinoma via NF-kB pathway","volume":"31","author":"Dai","year":"2013","journal-title":"Cancer Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"111150","DOI":"10.1016\/j.intimp.2023.111150","article-title":"Modulating intestinal barrier function by sphingosine-1-phosphate receptor 1 specific agonist SEW2871 attenuated ANIT-induced cholestatic hepatitis via the gut-liver axis","volume":"125","author":"Yang","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3109\/10799893.2014.986744","article-title":"Activation of the bile acid receptor TGR5 enhances LPS-induced inflammatory responses in a human monocytic cell line","volume":"35","author":"Mobraten","year":"2015","journal-title":"J. Recept. Signal Transduct. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.apsb.2021.07.011","article-title":"TGR5 deficiency activates antitumor immunity in non-small cell lung cancer via restraining M2 macrophage polarization","volume":"12","author":"Zhao","year":"2022","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"83951","DOI":"10.18632\/oncotarget.13796","article-title":"Chenodeoxycholic acid activates NLRP3 inflammasome and contributes to cholestatic liver fibrosis","volume":"7","author":"Gong","year":"2016","journal-title":"Oncotarget"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"109626","DOI":"10.1016\/j.intimp.2022.109626","article-title":"The FXR mediated anti-depression effect of CDCA underpinned its therapeutic potentiation for MDD","volume":"115","author":"Li","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Lai, Y.S., Nguyen, H.T., Salmanida, F.P., and Chang, K.T. (2021). MERTK+\/hi M2c Macrophages Induced by Baicalin Alleviate Non-Alcoholic Fatty Liver Disease. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910604"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1007\/s00011-020-01378-2","article-title":"Macrophage polarization and its role in the pathogenesis of acute lung injury\/acute respiratory distress syndrome","volume":"69","author":"Chen","year":"2020","journal-title":"Inflamm. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"100872","DOI":"10.1016\/j.jhepr.2023.100872","article-title":"24-Norursodeoxycholic acid ameliorates experimental alcohol-related liver disease and activates hepatic PPAR\u03b3","volume":"5","author":"Grander","year":"2023","journal-title":"JHEP Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.cbpb.2017.09.007","article-title":"Molecular cloning and characterization of farnesoid X receptor from large yellow croaker (Larimichthys crocea) and the effect of dietary CDCA on the expression of inflammatory genes in intestine and spleen","volume":"216","author":"Du","year":"2018","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1007\/s00011-022-01629-4","article-title":"Synergistic anti-inflammatory effect of gut microbiota and lithocholic acid on liver fibrosis","volume":"71","author":"Shao","year":"2022","journal-title":"Inflamm. Res."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Liu, J., Wei, Y., Jia, W., Can, C., Wang, R., Yang, X., Gu, C., Liu, F., Ji, C., and Ma, D. (2022). Chenodeoxycholic acid suppresses AML progression through promoting lipid peroxidation via ROS\/p38 MAPK\/DGAT1 pathway and inhibiting M2 macrophage polarization. Redox Biol., 56.","DOI":"10.1016\/j.redox.2022.102452"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Wu, W.J., Wang, S.H., Wu, C.C., Su, Y.A., Chiang, C.Y., Lai, C.H., Wang, T.H., Cheng, T.L., Kuo, J.Y., and Hsu, T.C. (2021). IL-4 and IL-13 Promote Proliferation of Mammary Epithelial Cells through STAT6 and IRS-1. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222112008"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/978-981-13-9867-4_3","article-title":"Chemical Components of Ganoderma","volume":"1181","author":"Gong","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1146\/annurev.immunol.17.1.701","article-title":"The IL-4 receptor: Signaling mechanisms and biologic functions","volume":"17","author":"Nelms","year":"1999","journal-title":"Annu. Rev. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1111\/imm.12173","article-title":"A critical role for suppressor of cytokine signalling 3 in promoting M1 macrophage activation and function in vitro and in vivo","volume":"141","author":"Arnold","year":"2014","journal-title":"Immunology"},{"key":"ref_57","first-page":"5305","article-title":"Chenodeoxycholic acid attenuates high-fat diet-induced obesity and hyperglycemia via the G protein-coupled bile acid receptor 1 and proliferator-activated receptor \u03b3 pathway","volume":"4","author":"Chen","year":"2017","journal-title":"Exp. Ther. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"121501","DOI":"10.1016\/j.lfs.2023.121501","article-title":"Taurohyodeoxycholic acid alleviates trinitrobenzene sulfonic acid induced ulcerative colitis via regulating Th1\/Th2 and Th17\/Treg cells balance","volume":"318","author":"Lv","year":"2023","journal-title":"Life Sci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Chen, Y.S., Liu, H.M., and Lee, T.Y. (2019). Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice. Cells, 8.","DOI":"10.3390\/cells8030253"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/1\/429\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:23:49Z","timestamp":1759919029000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/1\/429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":59,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["ijms26010429"],"URL":"https:\/\/doi.org\/10.3390\/ijms26010429","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,6]]}}}