{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:05:54Z","timestamp":1787065554819,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["20H00427"],"award-info":[{"award-number":["20H00427"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In semelparous species like the ayu (Plecoglossus altivelis), spawning is followed by rapid physiological decline and death; yet, the underlying molecular mechanisms remain largely unexplored. This study examines transcriptomic changes in ayu skeletal muscle before and after spawning, with a focus on key genes and pathways contributing to muscle atrophy and metabolic dysfunction. Through RNA sequencing and DEG analysis, we identified over 3000 DEGs, and GSEA and KEGG pathway analysis revealed significant downregulation of energy metabolism and protein degradation. In post-spawning ayu, a rapid decrease in body weight was observed, accompanied by a decline in the expression of myosin heavy chain genes, which are major muscle protein genes, and gene expression changes indicative of muscle atrophy. Decreased expression of AP-1 transcription factors associated with muscle development and aging was also evident. PPI network analysis identified carbohydrate catabolism protein gapdh may be the key factor that led to muscle atrophy and accelerated aging in ayu. Our study revealed that after spawning, the ayu muscle tissue undergoes strong metabolic disorders and cellular stress responses, providing special insights into the mechanisms through the post-spawning death of ayu.<\/jats:p>","DOI":"10.3390\/ijms26020434","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T03:38:41Z","timestamp":1736221121000},"page":"434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic Insights into Post-Spawning Death and Muscle Atrophy in Ayu (Plecoglossus altivelis)"],"prefix":"10.3390","volume":"26","author":[{"given":"Jiancheng","family":"Liang","sequence":"first","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo 113-8657, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Fujisawa","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo 113-8657, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Toma","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo 113-8657, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Asakawa","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo 113-8657, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8247-9443","authenticated-orcid":false,"given":"Kazutoshi","family":"Yoshitake","sequence":"additional","affiliation":[{"name":"School of Marine Biosciences, Kitasato University, Sagamihara 252-0373, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoji","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Graduate School of Bioresources, Mie University, 1577 Kurima-machiya, Tsu 514-8507, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Saito","sequence":"additional","affiliation":[{"name":"Gunma Prefectural Fisheries Experiment Station, 13 Shikishima, Maebashi 371-0036, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Akutsu","sequence":"additional","affiliation":[{"name":"Gunma Prefectural Fisheries Experiment Station, 13 Shikishima, Maebashi 371-0036, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyuma","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Gunma Prefectural Fisheries Experiment Station, 13 Shikishima, Maebashi 371-0036, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6458-1487","authenticated-orcid":false,"given":"Shigeharu","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo 113-8657, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0044-8486(02)00626-9","article-title":"The Influence of Rearing Density on Stress Response and Disease Susceptibility of Ayu (Plecoglossus altivelis)","volume":"220","author":"Iguchi","year":"2003","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S1096-4959(01)00501-2","article-title":"Preferential Accumulation of Fatty Acids in the Testis and Ovary of Cultured and Wild Sweet Smelt Plecoglossus altivelis","volume":"131","author":"Jeong","year":"2002","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.fsi.2011.03.005","article-title":"Identification and Characterization of a Novel Cathelicidin from Ayu, Plecoglossus altivelis","volume":"31","author":"Lu","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.aquaculture.2011.01.024","article-title":"Effects of Suspended Sediment on Cortisol Levels in Wild and Cultured Strains of Ayu Plecoglossus altivelis","volume":"314","author":"Awata","year":"2011","journal-title":"Aquaculture"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1749-7345.2003.tb00042.x","article-title":"Effects of Feeding Rate and Feeding Frequency on Survival, Growth, and Body Composition of Ayu Post-Larvae Plecoglossus altivelis","volume":"34","author":"Cho","year":"2003","journal-title":"J. World Aquac. Soc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"536","DOI":"10.2108\/zsj.26.536","article-title":"Ayu (Plecoglossus altivelis) in a Contact Zone Between Amphidromous and Landlocked Forms: Genetic Analyses of Populations in the Yodo River System","volume":"26","author":"Takeshima","year":"2009","journal-title":"Zoolog. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Inui, R., Akamatsu, Y., Kono, T., Saito, M., Miyazono, S., and Nakao, R. (2021). Spatiotemporal Changes of the Environmental DNA Concentrations of Amphidromous Fish Plecoglossus altivelis altivelis in the Spawning Grounds in the Takatsu River, Western Japan. Front. Ecol. Evol., 9.","DOI":"10.3389\/fevo.2021.622149"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"10896","DOI":"10.1007\/s11356-023-31743-7","article-title":"Population Structure and Demographic History for Year Cohort Dynamics of Landlocked Ayu Plecoglossus altivelis altivelis in Dam Reservoir of Japan","volume":"31","author":"Khatun","year":"2024","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.1111\/brv.12753","article-title":"Life-history Strategies in Salmonids: The Role of Physiology and Its Consequences","volume":"96","author":"Bordeleau","year":"2021","journal-title":"Biol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1046\/j.1365-2826.2003.01033.x","article-title":"Reproduction and Resistance to Stress: When and How","volume":"15","author":"Wingfield","year":"2003","journal-title":"J. Neuroendocrinol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1644\/07-MAMM-A-083.1","article-title":"Semelparity and Factors Affecting the Reproductive Activity of the Brazilian Slender Opossum (Marmosops paulensis) in Southeastern Brazil","volume":"89","author":"Leiner","year":"2008","journal-title":"J. Mammal."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1098\/rspb.2000.1369","article-title":"Semelparity in a Large Marsupial","volume":"268","author":"Oakwood","year":"2001","journal-title":"Proc. R. Soc. Lond. B Biol. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1002\/jez.a.279","article-title":"Increased Leptin May Be Involved in the Short Life Span of Ayu (Plecoglossus altivelis)","volume":"305A","author":"Nagasaka","year":"2006","journal-title":"J. Exp. Zoolog. A Comp. Exp. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S1050-4648(02)00136-5","article-title":"Flow Cytometric Analysis of the Neutrophil Respiratory Burst of Ayu, Plecoglossus altivelis: Comparison with Other Fresh Water Fish","volume":"15","author":"Moritomo","year":"2003","journal-title":"Fish Shellfish Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.exger.2005.05.003","article-title":"Effects of Caloric Restriction on Post-Spawning Death of Ayu","volume":"40","author":"Nagasaka","year":"2005","journal-title":"Exp. Gerontol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.mod.2015.02.006","article-title":"Regulation of Gene Expression Mediating Indeterminate Muscle Growth in Teleosts","volume":"137","author":"Ahammad","year":"2015","journal-title":"Mech. Dev."},{"key":"ref_17","first-page":"11","article-title":"The AP-1 Transcriptional Complex: Local Switch or Remote Command?","volume":"1872","author":"Bejjani","year":"2019","journal-title":"Biochim. Biophys. Acta BBA-Rev. Cancer"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.1038\/sj.onc.1204383","article-title":"AP-1 in Cell Proliferation and Survival","volume":"20","author":"Shaulian","year":"2001","journal-title":"Oncogene"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1007\/s10126-022-10153-9","article-title":"Age-Associated Different Transcriptome Profiling in Zebrafish and Rats: An Insight into the Diversity of Vertebrate Aging","volume":"24","author":"Kijima","year":"2022","journal-title":"Mar. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nakamoto, M., Uchino, T., Koshimizu, E., Kuchiishi, Y., Sekiguchi, R., Wang, L., Sudo, R., Endo, M., Guiguen, Y., and Schartl, M. (2021). A Y-Linked Anti-M\u00fcllerian Hormone Type-II Receptor Is the Sex-Determining Gene in Ayu, Plecoglossus altivelis. PLoS Genet., 17.","DOI":"10.1371\/journal.pgen.1009705"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/BF00265637","article-title":"The 65-kDa Cytosolic Protein Associated with Warm Temperature Acclimation in Goldfish, Carassius auratus","volume":"163","author":"Kikuchi","year":"1993","journal-title":"J. Comp. Physiol. B"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1023\/A:1007768531655","article-title":"The Wap65 Gene Expression of Goldfish (Carassius auratus) in Association with Warm Water Temperature as Well as Bacterial Lipopolysaccharide (LPS)","volume":"17","author":"Kikuchi","year":"1997","journal-title":"Fish Physiol. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1007\/s11033-009-9551-1","article-title":"Molecular Cloning of Liver Wap65 cDNA in Ayu (Plecoglossus altivelis) and mRNA Expression Changes Following Listonella Anguillarum Infection","volume":"37","author":"Shi","year":"2010","journal-title":"Mol. Biol. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.1182\/blood-2005-03-1185","article-title":"Identification of the Receptor Scavenging Hemopexin-Heme Complexes","volume":"106","author":"Hvidberg","year":"2005","journal-title":"Blood"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1007\/s10038-006-0408-3","article-title":"Periaxin Mutation in Japanese Patients with Charcot-Marie-Tooth Disease","volume":"51","author":"Otagiri","year":"2006","journal-title":"J. Hum. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1086\/318208","article-title":"Periaxin Mutations Cause Recessive Dejerine-Sottas Neuropathy","volume":"68","author":"Boerkoel","year":"2001","journal-title":"Am. J. Hum. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s12576-020-00768-9","article-title":"The Ubiquitin\u2013Proteasome System in Regulation of the Skeletal Muscle Homeostasis and Atrophy: From Basic Science to Disorders","volume":"70","author":"Kitajima","year":"2020","journal-title":"J. Physiol. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.neuron.2004.06.005","article-title":"The AP-1 Transcription Factor c-Jun Is Required for Efficient Axonal Regeneration","volume":"43","author":"Raivich","year":"2004","journal-title":"Neuron"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lyu, P., Settlage, R.E., and Jiang, H. (2021). Genome-Wide Identification of Enhancers and Transcription Factors Regulating the Myogenic Differentiation of Bovine Satellite Cells. BMC Genomics, 22.","DOI":"10.1186\/s12864-021-08224-7"},{"key":"ref_30","first-page":"5772089","article-title":"Overexpression of Activating Transcription Factor-2 (ATF-2) Activates Wnt\/Ca2+ Signaling Pathways and Promotes Proliferation and Invasion in Non-Small-Cell Lung Cancer","volume":"2022","author":"Zhang","year":"2022","journal-title":"Dis. Markers"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1007\/s00109-009-0520-x","article-title":"ATF3 Transcription Factor and Its Emerging Roles in Immunity and Cancer","volume":"87","author":"Thompson","year":"2009","journal-title":"J. Mol. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/s41374-020-00516-y","article-title":"ATF-3 Expression Inhibits Melanoma Growth by Downregulating ERK and AKT Pathways","volume":"101","author":"Zu","year":"2021","journal-title":"Lab. Investig."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Sobolev, V.V., Khashukoeva, A.Z., Evina, O.E., Geppe, N.A., Chebysheva, S.N., Korsunskaya, I.M., Tchepourina, E., and Mezentsev, A. (2022). Role of the Transcription Factor FOSL1 in Organ Development and Tumorigenesis. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031521"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.cellsig.2010.08.003","article-title":"The Diverse Functions of GAPDH: Views from Different Subcellular Compartments","volume":"23","author":"Tristan","year":"2011","journal-title":"Cell. Signal."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/j.1439-0426.2006.00740.x","article-title":"Elevated Levels of Oxidative DNA Damage Activate P53 and Caspases in Brain of Ayu with Aging","volume":"22","author":"Nagasaka","year":"2006","journal-title":"J. Appl. Ichthyol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/S0891-5849(01)00719-5","article-title":"NF\u03baB and AP-1 Mediate Transcriptional Responses to Oxidative Stress in Skeletal Muscle Cells","volume":"31","author":"Zhou","year":"2001","journal-title":"Free Radic. Biol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1161\/CIRCRESAHA.120.317132","article-title":"Hyperglycemia Induces Myocardial Dysfunction via Epigenetic Regulation of JunD","volume":"127","author":"Hussain","year":"2020","journal-title":"Circ. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.mad.2010.03.009","article-title":"Age- and Tissue-Specific Changes in Mitochondrial and Nuclear DNA Base Excision Repair Activity in Mice: Susceptibility of Skeletal Muscles to Oxidative Injury","volume":"131","author":"Szczesny","year":"2010","journal-title":"Mech. Ageing Dev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.cbpa.2009.02.027","article-title":"Corticosteroids: Friends or Foes of Teleost Fish Reproduction?","volume":"153","author":"Milla","year":"2009","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1086\/689994","article-title":"Glucocorticoids in Fish Eggs: Variation, Interactions with the Environment, and the Potential to Shape Offspring Fitness","volume":"90","author":"Sopinka","year":"2017","journal-title":"Physiol. Biochem. Zool."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1152\/physrev.1997.77.3.591","article-title":"The Stress Response in Fish","volume":"77","year":"1997","journal-title":"Physiol. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/jfb.13904","article-title":"Measuring Cortisol, the Major Stress Hormone in Fishes","volume":"94","author":"Sadoul","year":"2019","journal-title":"J. Fish Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106416","DOI":"10.1016\/j.fishres.2022.106416","article-title":"Scope of the Cortisol Stress Response in Chinook Salmon during Maturation","volume":"254","author":"Couch","year":"2022","journal-title":"Fish. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Braun, T.P., and Marks, D.L. (2015). The Regulation of Muscle Mass by Endogenous Glucocorticoids. Front. Physiol., 6.","DOI":"10.3389\/fphys.2015.00012"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Yiallouris, A., Tsioutis, C., Agapidaki, E., Zafeiri, M., Agouridis, A.P., Ntourakis, D., and Johnson, E.O. (2019). Adrenal Aging and Its Implications on Stress Responsiveness in Humans. Front. Endocrinol., 10.","DOI":"10.3389\/fendo.2019.00054"},{"key":"ref_46","unstructured":"Heaselgrave, S.R. (2023). Glucocorticoid Excess, the NAD+ Metabolome and Energy Metabolism. [Ph.D. Thesis, University of Birmingham]."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-Based Genome Alignment and Genotyping with HISAT2 and HISAT-Genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map Format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Kovaka, S., Zimin, A.V., Pertea, G.M., Razaghi, R., Salzberg, S.L., and Pertea, M. (2019). Transcriptome Assembly from Long-Read RNA-Seq Alignments with StringTie2. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1910-1"},{"key":"ref_50","unstructured":"Dainat, J., Here\u00f1\u00fa, D., and Pucholt, P. (2020). \u201cAGAT: Another Gff Analysis Toolkit to Handle Annotations in Any GTF.\u201d GFF Format, Zenodo."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: A Flexible Suite of Utilities for Comparing Genomic Features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e211","DOI":"10.1002\/imt2.211","article-title":"JCVI: A Versatile Toolkit for Comparative Genomics Analysis","volume":"3","author":"Tang","year":"2024","journal-title":"iMeta"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The Zebrafish Reference Genome Sequence and Its Relationship to the Human Genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_56","unstructured":"Blighe, K., Rana, S., and Lewis, M. (2021, July 23). \u201cEnhancedVolcano: Publication-Ready Volcano Plots with Enhanced Colouring and Labeling\u201d. Available online: https:\/\/github.com\/kevinblighe\/EnhancedVolcano."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"W199","DOI":"10.1093\/nar\/gkz401","article-title":"WebGestalt 2019: Gene Set Analysis Toolkit with Revamped UIs and APIs","volume":"47","author":"Liao","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING Database in 2023: Protein\u2013Protein Association Networks and Functional Enrichment Analyses for Any Sequenced Genome of Interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/2\/434\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:23:57Z","timestamp":1759919037000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/2\/434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,7]]},"references-count":58,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["ijms26020434"],"URL":"https:\/\/doi.org\/10.3390\/ijms26020434","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,7]]}}}