{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:07:43Z","timestamp":1780376863978,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hainan Province\u2019s Key Research and Development Project","award":["ZDYF2024SHFZ061"],"award-info":[{"award-number":["ZDYF2024SHFZ061"]}]},{"name":"Hainan Province\u2019s Key Research and Development Project","award":["32271577"],"award-info":[{"award-number":["32271577"]}]},{"name":"Hainan Province\u2019s Key Research and Development Project","award":["32471587"],"award-info":[{"award-number":["32471587"]}]},{"name":"National Natural Science Foundation of China","award":["ZDYF2024SHFZ061"],"award-info":[{"award-number":["ZDYF2024SHFZ061"]}]},{"name":"National Natural Science Foundation of China","award":["32271577"],"award-info":[{"award-number":["32271577"]}]},{"name":"National Natural Science Foundation of China","award":["32471587"],"award-info":[{"award-number":["32471587"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Taurine is essential for sustaining the body\u2019s physiological equilibrium and is extensively utilized as a dietary supplement and immune system enhancer for aquatic creatures. The gut microbiota serves as a vital health indicator in animals. In this study, our goal was to evaluate the effects of dietary taurine on the gut microbiome of Chinese stripe-necked turtles (Mauremys sinensis). Turtles were evenly divided into three supplementation groups: a control group with no taurine in the diet, a low group with 0.1% taurine, and a high group with 0.4% taurine. High-throughput sequencing was employed to analyze the gut microbiome\u2019s composition and structure. The results showed that the impact of taurine supplementation on the \u03b1-diversity and \u03b2-diversity of the gut microbiome was not statistically significant. At the phylum level, Firmicutes and Bacteroidetes predominated the gut microbiome. At the genus level, three beneficial bacteria, Clostridium_sensu_stricto_13, Pygmaiobacter, and Terrisporobacter showed significant differences under different levels of taurine supplementation and increased in abundance with the supplementation of taurine, while the harmful bacterium Brucella decreased in abundance with the addition of taurine and exhibited significant differences. Furthermore, LEfSe analysis and functional predictions highlighted significant variations in the functional traits of gram-positive intestinal bacteria among the different taurine supplementation levels. These findings imply that dietary taurine may alter the intestinal microbiome structure in Chinese stripe-necked turtles, offering valuable insights for the scientific assessment of taurine\u2019s health benefits as a feed additive and potentially guiding the formulation of more informed and healthier feeding practices.<\/jats:p>","DOI":"10.3390\/ijms26020445","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T05:06:34Z","timestamp":1736226394000},"page":"445","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dietary Taurine Regulation of the Intestinal Microbiome in Chinese Stripe-Necked Turtle (Mauremys sinensis)"],"prefix":"10.3390","volume":"26","author":[{"given":"Yue","family":"Yuan","sequence":"first","affiliation":[{"name":"Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Niu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenguang","family":"Hao","sequence":"additional","affiliation":[{"name":"Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyue","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubin","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Hong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-5333","authenticated-orcid":false,"given":"Li","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Miles, A.R., Hawrysh, P.J., Hossein-Javaheri, N., and Buck, L.T. 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