{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:08:28Z","timestamp":1792181308503,"version":"4.4.0"},"reference-count":52,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Regional Ministry of Innovation, Universities, Science and Digital Society of the Generalitat Valenciana","award":["CIGE\/2023\/12"],"award-info":[{"award-number":["CIGE\/2023\/12"]}]},{"name":"Mar\u00edn-Garc\u00eda Pablo Jes\u00fas","award":["CIGE\/2023\/12"],"award-info":[{"award-number":["CIGE\/2023\/12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To investigate the impact of age on the metabolomic profile of loggerhead sea turtles (Caretta caretta), this study analyzed 100 plasma samples of individuals across two age groups\u201450 post-hatchlings and 50 juveniles\u2014from various locations along the Mediterranean coastline. Both targeted and untargeted metabolomic analyses were performed on the samples. Our results demonstrated a significant age-related effect on the metabolomic profiles in both analyses. Specifically, post-hatchling turtles exhibited increased levels of urea (p &lt; 0.001), triglyceride (p = 0.0003), cholesterol (p &lt; 0.001), lysoPE (18:1\/0:0) (p &lt; 0.001), 7-HDoHE (p = 0.0121), pyrrolidinebutanoic acid (p &lt; 0.001), formiminoglutamic acid (p &lt; 0.001), pyroglutamic acid (p &lt; 0.001), lysoPC (0:0\/20:4) (p &lt; 0.001), lysoPE (22:6\/0:0) (p &lt; 0.001), 1-acyl-sn-glycero-3-phosphocholine (p &lt; 0.001), DL-homocysteine (p &lt; 0.001) and gamma-Glutamyltyrosine (p &lt; 0.001). Conversely, post-hatchlings showed reduced levels of total protein (p &lt; 0.001), glucose (p = 0.0002), uric acid (p &lt; 0.001), inorganic phosphorus (p = 0.0018) and calcium (p = 0.0410) compared with juveniles. These findings suggest significant physiological changes between the age groups, likely due to differentiated feeding patterns. Further research is needed to better understand the metabolic profiles and complex physiological and nutritional interactions of this species.<\/jats:p>","DOI":"10.3390\/ijms26020545","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T06:25:54Z","timestamp":1736490354000},"page":"545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ecometabolomics of Loggerhead Sea Turtles (Caretta caretta): The Impact of Age on Metabolomic Profiles"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9593-3476","authenticated-orcid":false,"given":"Pablo Jes\u00fas","family":"Mar\u00edn-Garc\u00eda","sequence":"first","affiliation":[{"name":"Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA), Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3335-5831","authenticated-orcid":false,"given":"Daniel","family":"Garc\u00eda-P\u00e1rraga","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Oceanogr\u00e0fic de la Comunitat Valenciana, Ciudad de las Artes y las Ciencias, 46013 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Luis","family":"Crespo-Picazo","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Oceanogr\u00e0fic de la Comunitat Valenciana, Ciudad de las Artes y las Ciencias, 46013 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9806-9174","authenticated-orcid":false,"given":"Lola","family":"Llobat","sequence":"additional","affiliation":[{"name":"Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA), Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Cambra-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Institute for Animal Science and Technology, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Bordignon","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell\u2019Universit\u00e0 16, 35020 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7224-9978","authenticated-orcid":false,"given":"Juan Jos\u00e9","family":"Pascual","sequence":"additional","affiliation":[{"name":"Institute for Animal Science and Technology, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torben","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Sciences, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1164-4405","authenticated-orcid":false,"given":"Mette Skou","family":"Hedemann","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Sciences, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1080\/02757540903062517","article-title":"Ecological Metabolomics","volume":"25","author":"Sardans","year":"2009","journal-title":"Chem. 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