{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:38:05Z","timestamp":1759970285528,"version":"build-2065373602"},"reference-count":75,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T00:00:00Z","timestamp":1736553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Educaci\u00f3n y Ciencia, Spain","award":["SAF2008-00442","PR26\/16-20317","GR29\/20","21"],"award-info":[{"award-number":["SAF2008-00442","PR26\/16-20317","GR29\/20","21"]}]},{"name":"Universidad Complutense de Madrid","award":["SAF2008-00442","PR26\/16-20317","GR29\/20","21"],"award-info":[{"award-number":["SAF2008-00442","PR26\/16-20317","GR29\/20","21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Melatonin is involved in various functions such as the timing of circadian rhythms, energy metabolism, and body mass gain in experimental animals. However, its effects on adipose tissue lipid metabolism are still unclear. This study analyzes the effects of melatonin on the relative gene expression of lipolytic proteins in rat mesenteric adipose tissue and free fatty acid (FFA) and glycerol plasma levels of male Wistar rats fed a high-fat (HFD) or maintenance diet. Four experimental groups were established: control, obese, and control or obese plus 2.3 mg\/kg\/day of melatonin in tap water. After 11 weeks, animals were sacrificed at different times throughout a 24 h cycle, and mesenteric adipose tissue and plasma samples were collected and analyzed. Cgi58, Perilipin, and Dgat1 gene expression, as well as FFA and glycerol concentrations, showed rhythm patterns in the control group. HFD disrupted those rhythm patterns and increased FFA and glycerol concentrations during the dark photoperiod. In both melatonin-treated groups, almost all analyzed genes showed circadian patterns. Notably, melatonin significantly prevented the increase in FFA levels during the dark photoperiod in obese rats (obese group: ~1100 mM vs. obese + melatonin group: ~600 \u03bcM, similar to control levels). However, the rhythmic pattern observed in control animals was not sustained. According to our results, melatonin could regulate circadian gene transcription of mesenteric adipose tissue lipolysis proteins. The effect of melatonin on preventing elevated FFA plasma levels associated with high-fat diet intake warrants further investigation.<\/jats:p>","DOI":"10.3390\/ijms26020577","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T07:40:08Z","timestamp":1736754008000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8770-2723","authenticated-orcid":false,"given":"Pilar","family":"Cano-Barquilla","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanesa","family":"Jim\u00e9nez-Ortega","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4113-4756","authenticated-orcid":false,"given":"Pilar","family":"Fern\u00e1ndez-Mateos","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), 28003 Madrid, Spain"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3376-5232","authenticated-orcid":false,"given":"Leire","family":"Virto","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), 28003 Madrid, Spain"},{"name":"Departamento de Anatom\u00eda y Embriolog\u00eda, Facultad de \u00d3ptica y Optometr\u00eda, Universidad Complutense de Madrid, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1982-6526","authenticated-orcid":false,"given":"Estela","family":"Maldonado Bautista","sequence":"additional","affiliation":[{"name":"Departamento de Anatom\u00eda y Embriolog\u00eda, Faculta de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-5248","authenticated-orcid":false,"given":"Juliana","family":"Perez-Miguelsanz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), 28003 Madrid, Spain"},{"name":"Departamento de Anatom\u00eda y Embriolog\u00eda, Faculta de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana I.","family":"Esquifino","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Facultad de Medicina, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), 28003 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2012.10.001","article-title":"Adipose Tissue Heterogeneity: Implication of Depot Differences in Adipose Tissue for Obesity Complications","volume":"34","author":"Lee","year":"2013","journal-title":"Mol. Aspects Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.cmet.2013.03.008","article-title":"Mechanisms and Metabolic Implications of Regional Differences among Fat Depots","volume":"17","author":"Tchkonia","year":"2013","journal-title":"Cell Metab."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Perez-Miguelsanz, J., Jim\u00e9nez-Ortega, V., Cano-Barquilla, P., Garaulet, M., Esquifino, A.I., Varela-Moreiras, G., and Fern\u00e1ndez-Mateos, P. (2021). Early Appearance of Epicardial Adipose Tissue through Human Development. Nutrients, 13.","DOI":"10.3390\/nu13092906"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Santillana, N., Astudillo-Guerrero, C., D\u2019Espessailles, A., and Cruz, G. (2023). White Adipose Tissue Dysfunction: Pathophysiology and Emergent Measurements. Nutrients, 15.","DOI":"10.3390\/nu15071722"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.tips.2015.04.014","article-title":"Adipokines in Health and Disease","volume":"36","author":"Fasshauer","year":"2015","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.tem.2010.01.009","article-title":"Gluttony, Sloth and the Metabolic Syndrome: A Roadmap to Lipotoxicity","volume":"21","author":"Unger","year":"2010","journal-title":"Trends Endocrinol. Metab. TEM"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3190","DOI":"10.2337\/diabetes.54.11.3190","article-title":"Adipocyte Lipases and Defect of Lipolysis in Human Obesity","volume":"54","author":"Langin","year":"2005","journal-title":"Diabetes"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1038\/s42255-021-00493-6","article-title":"Lipolysis: Cellular Mechanisms for Lipid Mobilization from Fat Stores","volume":"3","author":"Grabner","year":"2021","journal-title":"Nat. Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.tem.2005.01.009","article-title":"A New Lipase in Regulating Lipid Mobilization: Hormone-Sensitive Lipase Is Not Alone","volume":"16","author":"Raben","year":"2005","journal-title":"Trends Endocrinol. Metab. TEM"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.cmet.2006.03.005","article-title":"Adipose Triglyceride Lipase-Mediated Lipolysis of Cellular Fat Stores Is Activated by CGI-58 and Defective in Chanarin-Dorfman Syndrome","volume":"3","author":"Lass","year":"2006","journal-title":"Cell Metab."},{"key":"ref_11","first-page":"221","article-title":"Adipose Triglyceride Lipase Regulation: An Overview","volume":"19","author":"Cerk","year":"2018","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4752","DOI":"10.1111\/j.1742-4658.2009.07172.x","article-title":"Phosphorylation of Hormone-Sensitive Lipase by Protein Kinase A in Vitro Promotes an Increase in Its Hydrophobic Surface Area","volume":"276","author":"Krintel","year":"2009","journal-title":"FEBS J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101084","DOI":"10.1016\/j.plipres.2020.101084","article-title":"Hormone-Sensitive Lipase: Sixty Years Later","volume":"82","author":"Recazens","year":"2021","journal-title":"Prog. Lipid Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1194\/jlr.M093112","article-title":"The Triglyceride Synthesis Enzymes DGAT1 and DGAT2 Have Distinct and Overlapping Functions in Adipocytes","volume":"60","author":"Chitraju","year":"2019","journal-title":"J. Lipid Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"R67","DOI":"10.1530\/JOE-17-0002","article-title":"FoxO Integration of Insulin Signaling with Glucose and Lipid Metabolism","volume":"233","author":"Lee","year":"2017","journal-title":"J. Endocrinol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Xin, H., Zhang, J., Huang, R., Li, L., Lam, S.M., Shui, G., Deng, F., Zhang, Z., and Li, M.-D. (2022). Circadian Signatures of Adipose Tissue in Diet-Induced Obesity. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.953237"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102114","DOI":"10.1016\/j.gde.2023.102114","article-title":"Adipose Tissue Lipid Metabolism: Lipolysis","volume":"83","author":"Cho","year":"2023","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.2337\/db12-1449","article-title":"Circadian Regulation of Lipid Mobilization in White Adipose Tissues","volume":"62","author":"Shostak","year":"2013","journal-title":"Diabetes"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1152\/physrev.00063.2017","article-title":"Mechanisms of Insulin Action and Insulin Resistance","volume":"98","author":"Petersen","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Civelek, E., Ozturk Civelek, D., Akyel, Y.K., Kaleli Durman, D., and Okyar, A. (2023). Circadian Dysfunction in Adipose Tissue: Chronotherapy in Metabolic Diseases. Biology, 12.","DOI":"10.3390\/biology12081077"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.neuron.2012.04.006","article-title":"Timing to Perfection: The Biology of Central and Peripheral Circadian Clocks","volume":"74","author":"Albrecht","year":"2012","journal-title":"Neuron"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3956","DOI":"10.1038\/s41467-022-31525-8","article-title":"Lipolysis Regulates Major Transcriptional Programs in Brown Adipocytes","volume":"13","author":"Markussen","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e504","DOI":"10.1002\/mco2.504","article-title":"The Role of Circadian Clock in Regulating Cell Functions: Implications for Diseases","volume":"5","author":"Lin","year":"2024","journal-title":"MedComm"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4161\/adip.26007","article-title":"Circadian Regulation of Adipose Function","volume":"2","author":"Shostak","year":"2013","journal-title":"Adipocyte"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.2337\/db13-0457","article-title":"A Novel Link Between Circadian Clocks and Adipose Tissue Energy Metabolism","volume":"62","author":"Yoshino","year":"2013","journal-title":"Diabetes"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"R432","DOI":"10.1016\/j.cub.2016.04.011","article-title":"The Circadian Clock and Human Health","volume":"26","author":"Roenneberg","year":"2016","journal-title":"Curr. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/978-3-031-63657-8_2","article-title":"Misalignment of Circadian Rhythms in Diet-Induced Obesity","volume":"1460","author":"Engin","year":"2024","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1600-079X.2011.00934.x","article-title":"Melatonin, the Circadian Multioscillator System and Health: The Need for Detailed Analyses of Peripheral Melatonin Signaling","volume":"52","author":"Hardeland","year":"2012","journal-title":"J. Pineal Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e13701","DOI":"10.1111\/obr.13701","article-title":"Meta-Analysis and Machine Learning Reveal the Antiobesity Effects of Melatonin on Obese Rodents","volume":"25","author":"Li","year":"2024","journal-title":"Obes. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Owino, S., Buonfiglio, D.D.C., Tchio, C., and Tosini, G. (2019). Melatonin Signaling a Key Regulator of Glucose Homeostasis and Energy Metabolism. Front. Endocrinol., 10.","DOI":"10.3389\/fendo.2019.00488"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Mateos, P., Cano-Barquilla, P., Jim\u00e9nez-Ortega, V., Virto, L., P\u00e9rez-Miguelsanz, J., and Esquifino, A.I. (2023). Effect of Melatonin on Redox Enzymes Daily Gene Expression in Perirenal and Subcutaneous Adipose Tissue of a Diet Induced Obesity Model. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24020960"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e12961","DOI":"10.1111\/jpi.12961","article-title":"Melatonin in Energy Control: Circadian Time-Giver and Homeostatic Monitor","volume":"76","author":"Challet","year":"2024","journal-title":"J. Pineal Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/j.1600-079X.2010.00798.x","article-title":"Melatonin Effect on Plasma Adiponectin, Leptin, Insulin, Glucose, Triglycerides and Cholesterol in Normal and High Fat\u2013Fed Rats","volume":"49","author":"Cano","year":"2010","journal-title":"J. Pineal Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1111\/jpi.12137","article-title":"Melatonin, Energy Metabolism, and Obesity: A Review","volume":"56","author":"Amaral","year":"2014","journal-title":"J. Pineal Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e12549","DOI":"10.1111\/jpi.12549","article-title":"Melatonin Multiple Effects on Brown Adipose Tissue Molecular Machinery","volume":"66","author":"Gallo","year":"2019","journal-title":"J. Pineal Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Guan, Q., Wang, Z., Cao, J., Dong, Y., and Chen, Y. (2021). Mechanisms of Melatonin in Obesity: A Review. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010218"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1111\/jpn.13497","article-title":"Effect of Melatonin on Visceral Fat Deposition, Lipid Metabolism and Hepatic Lipo-Metabolic Gene Expression in Male Rats","volume":"105","author":"Wang","year":"2021","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/s41387-022-00222-2","article-title":"Suppression of Obesity by Melatonin through Increasing Energy Expenditure and Accelerating Lipolysis in Mice Fed a High-Fat Diet","volume":"12","author":"Xu","year":"2022","journal-title":"Nutr. Diabetes"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.1210\/endo.142.10.8423","article-title":"Functional Expression of MT2 (Mel1b) Melatonin Receptors in Human PAZ6 Adipocytes","volume":"142","author":"Brydon","year":"2001","journal-title":"Endocrinology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e12702","DOI":"10.1111\/jpi.12702","article-title":"Melatonin Controversies, an Update","volume":"70","author":"Boutin","year":"2021","journal-title":"J. Pineal Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.tem.2019.11.011","article-title":"Melatonin Effects on Glucose Metabolism: Time to Unlock the Controversy","volume":"31","author":"Garaulet","year":"2020","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1113\/JP276488","article-title":"Interplay between Diet, Exercise and the Molecular Circadian Clock in Orchestrating Metabolic Adaptations of Adipose Tissue","volume":"597","author":"Dollet","year":"2019","journal-title":"J. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"38870","DOI":"10.1074\/jbc.M106219200","article-title":"Cloning of DGAT2, a Second Mammalian Diacylglycerol Acyltransferase, and Related Family Members","volume":"276","author":"Cases","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1038\/oby.2002.130","article-title":"Fat Intake Affects Adiposity, Comorbidity Factors, and Energy Metabolism of Sprague-Dawley Rats","volume":"10","author":"Ghibaudi","year":"2002","journal-title":"Obes. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1210\/jcem-72-1-96","article-title":"The Role of Free Fatty Acid Metabolism in the Pathogenesis of Insulin Resistance in Obesity and Noninsulin-Dependent Diabetes Mellitus","volume":"72","author":"Groop","year":"1991","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"G659","DOI":"10.1152\/ajpgi.00079.2018","article-title":"Mesenteric Adipose Tissue Contributes to Intestinal Barrier Integrity and Protects against Nonalcoholic Fatty Liver Disease in Mice","volume":"315","author":"Wu","year":"2018","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.1038\/oby.2009.200","article-title":"Effect of a High-Fat Diet on 24-Hour Pattern of Circulating Adipocytokines in Rats","volume":"17","author":"Cano","year":"2009","journal-title":"Obesity"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Chandrasekaran, P., and Weiskirchen, R. (2024). The Role of Obesity in Type 2 Diabetes Mellitus\u2014An Overview. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25031882"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Bast\u00edas-P\u00e9rez, M., Serra, D., and Herrero, L. (2020). Dietary Options for Rodents in the Study of Obesity. Nutrients, 12.","DOI":"10.3390\/nu12113234"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/978-3-319-48382-5_2","article-title":"Circadian Rhythms in Diet-Induced Obesity","volume":"960","author":"Engin","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/s41574-020-0318-z","article-title":"Food as a Circadian Time Cue\u2014Evidence from Human Studies","volume":"16","author":"Lewis","year":"2020","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_52","first-page":"549","article-title":"Restricted Feeding Modulates Peripheral Clocks and Nutrient Sensing Pathways in Rats","volume":"65","author":"Rodrigues","year":"2021","journal-title":"Arch. Endocrinol. Metab."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1016\/j.cell.2013.11.034","article-title":"Reprogramming of the Circadian Clock by Nutritional Challenge","volume":"155","author":"Patel","year":"2013","journal-title":"Cell"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Li, Y., Li, Z., Ngandiri, D.A., Llerins Perez, M., Wolf, A., and Wang, Y. (2022). The Molecular Brakes of Adipose Tissue Lipolysis. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.826314"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.devcel.2013.01.013","article-title":"Triacylglycerol Synthesis Enzymes Mediate Lipid Droplet Growth by Relocalizing from the ER to Lipid Droplets","volume":"24","author":"Wilfling","year":"2013","journal-title":"Dev. Cell"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.clinthera.2022.12.008","article-title":"Inhibition of Diacylglycerol Acyltransferase 2 Versus Diacylglycerol Acyltransferase 1: Potential Therapeutic Implications of Pharmacology","volume":"45","author":"Amin","year":"2023","journal-title":"Clin. Ther."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.orcp.2023.09.010","article-title":"Rat Models of Diet-Induced Obesity and Metabolic Dysregulation: Current Trends, Shortcomings and Considerations for Future Research","volume":"17","year":"2023","journal-title":"Obes. Res. Clin. Pract."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Yang, W., Tang, K., Wang, Y., Zhang, Y., and Zan, L. (2017). Melatonin Promotes Triacylglycerol Accumulation via MT2 Receptor during Differentiation in Bovine Intramuscular Preadipocytes. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-12780-y"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1111\/j.1600-079X.2008.00610.x","article-title":"Melatonin and the Circadian Entrainment of Metabolic and Hormonal Activities in Primary Isolated Adipocytes","volume":"45","author":"Andreotti","year":"2008","journal-title":"J. Pineal Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e12383","DOI":"10.1111\/jpi.12383","article-title":"Melatonin Promotes Circadian Rhythm-Induced Proliferation through Clock\/Histone Deacetylase 3\/c-Myc Interaction in Mouse Adipose Tissue","volume":"62","author":"Liu","year":"2017","journal-title":"J. Pineal Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/jpi.12211","article-title":"Pinealectomy Interferes with the Circadian Clock Genes Expression in White Adipose Tissue","volume":"58","author":"Andreotti","year":"2015","journal-title":"J. Pineal Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/jpi.12259","article-title":"Melatonin Promotes Adipogenesis and Mitochondrial Biogenesis in 3T3-L1 Preadipocytes","volume":"59","author":"Kato","year":"2015","journal-title":"J. Pineal Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3382\/ps\/pev315","article-title":"Effects of Abhydrolase Domain Containing 5 Gene (ABHD5) Expression and Variations on Chicken Fat Metabolism","volume":"95","author":"Ouyang","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"de Farias, T.d.S.M., Cruz, M.M., de Sa, R.C.d.C., Severi, I., Perugini, J., Senzacqua, M., Cerutti, S.M., Giordano, A., Cinti, S., and Alonso-Vale, M.I.C. (2019). Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice. Front. Endocrinol., 10.","DOI":"10.3389\/fendo.2019.00750"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1194\/jlr.R700014-JLR200","article-title":"Thematic Review Series: Adipocyte Biology. The Perilipin Family of Structural Lipid Droplet Proteins: Stabilization of Lipid Droplets and Control of Lipolysis","volume":"48","author":"Brasaemle","year":"2007","journal-title":"J. Lipid Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5347","DOI":"10.1210\/en.2003-0693","article-title":"Melatonin Reduces Body Weight Gain in Sprague Dawley Rats with Diet-Induced Obesity","volume":"144","author":"Desbazeille","year":"2003","journal-title":"Endocrinology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.physbeh.2013.04.006","article-title":"Melatonin Reduces Body Weight Gain and Increases Nocturnal Activity in Male Wistar Rats","volume":"118","author":"Pariente","year":"2013","journal-title":"Physiol. Behav."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e12623","DOI":"10.1111\/jpi.12623","article-title":"Melatonin Ameliorates SGLT2 Inhibitor-Induced Diabetic Ketoacidosis by Inhibiting Lipolysis and Hepatic Ketogenesis in Type 2 Diabetic Mice","volume":"68","author":"Park","year":"2020","journal-title":"J. Pineal Res."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Percie du Sert, N., Hurst, V., Ahluwalia, A., Alam, S., Avey, M.T., Baker, M., Browne, W.J., Clark, A., Cuthill, I.C., and Dirnagl, U. (2020). The ARRIVE Guidelines 2.0: Updated Guidelines for Reporting Animal Research. PLoS Biol., 18.","DOI":"10.1371\/journal.pbio.3000410"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1111\/jpi.12168","article-title":"Melatonin Normalizes Clinical and Biochemical Parameters of Mild Inflammation in Diet-Induced Metabolic Syndrome in Rats","volume":"57","author":"Pagano","year":"2014","journal-title":"J. Pineal Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1515\/hmbci-2014-0041","article-title":"Melatonin Counteracts Changes in Hypothalamic Gene Expression of Signals Regulating Feeding Behavior in High-Fat Fed Rats","volume":"21","author":"Mateos","year":"2015","journal-title":"Horm. Mol. Biol. Clin. Investig."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1194\/jlr.D023788","article-title":"Adiposoft: Automated Software for the Analysis of White Adipose Tissue Cellularity in Histological Sections","volume":"53","author":"Galarraga","year":"2012","journal-title":"J. Lipid Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1038\/ijo.2017.90","article-title":"Grape Seed Proanthocyanidin Supplementation Reduces Adipocyte Size and Increases Adipocyte Number in Obese Rats","volume":"41","author":"Bravo","year":"2017","journal-title":"Int. J. Obes."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"van der Spek, R., Fliers, E., la Fleur, S.E., and Kalsbeek, A. (2018). Daily Gene Expression Rhythms in Rat White Adipose Tissue Do Not Differ Between Subcutaneous and Intra-Abdominal Depots. Front. Endocrinol., 9.","DOI":"10.3389\/fendo.2018.00206"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/2\/577\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:26:54Z","timestamp":1759919214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/2\/577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,11]]},"references-count":75,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["ijms26020577"],"URL":"https:\/\/doi.org\/10.3390\/ijms26020577","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2025,1,11]]}}}