{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:39:14Z","timestamp":1759970354353,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2023YFD2400701","32273181","2024B03J1263","2023TD48"],"award-info":[{"award-number":["2023YFD2400701","32273181","2024B03J1263","2023TD48"]}]},{"name":"National Natural Science Fund","award":["2023YFD2400701","32273181","2024B03J1263","2023TD48"],"award-info":[{"award-number":["2023YFD2400701","32273181","2024B03J1263","2023TD48"]}]},{"name":"Key Research and Development Program of Guangzhou City","award":["2023YFD2400701","32273181","2024B03J1263","2023TD48"],"award-info":[{"award-number":["2023YFD2400701","32273181","2024B03J1263","2023TD48"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","award":["2023YFD2400701","32273181","2024B03J1263","2023TD48"],"award-info":[{"award-number":["2023YFD2400701","32273181","2024B03J1263","2023TD48"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Glutamine metabolism is essential for infectious spleen and kidney necrosis virus (ISKNV) replication. Glutaminase 1 (GLS1), the key enzyme of the glutamine metabolism, and c-Myc positively regulate ISKNV infection, while c-Myc is closely correlated with GLS1. However, the regulatory mechanism among ISKNV, c-Myc and glutamine metabolism remains unclear. Here, we indicated that c-Myc increased glutamine uptake by increasing the GLS1, glutamate dehydrogenase (GDH) and isocitrate dehydrogenase (IDH2) expression of glutamine metabolism. ISKNV ORF102R, ORF093R and ORF118L co-located with c-Myc in CPB cells. Co-IP results showed that ISKNV ORF102R and ORF093R interacted with c-Myc, while ORF118L did not interact with c-Myc. The expression levels of c-Myc, GLS1 and IDH2 were increased in ISKNV ORF093R expression cells, and the mRNA and protein levels of GLS1 were upregulated in ISKNV 102R-expressing cells. These results indicated that ISKNV reconstructed glutamine metabolism to satisfy the energy and macromolecule requirements for virus proliferation by ORF093R and ORF102R interacting with c-Myc, which provides the foundation for innovative antiviral strategies.<\/jats:p>","DOI":"10.3390\/ijms26020718","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T06:46:10Z","timestamp":1737009970000},"page":"718","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Infectious Spleen and Kidney Necrosis Virus ORF093R and ORF102R Regulate Glutamate Metabolic Reprogramming to Support Virus Proliferation by Interacting with c-Myc"],"prefix":"10.3390","volume":"26","author":[{"given":"Yinjie","family":"Niu","sequence":"first","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caimei","family":"Ye","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6476-1704","authenticated-orcid":false,"given":"Qiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongru","family":"Liang","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Luo","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4591-3577","authenticated-orcid":false,"given":"Baofu","family":"Ma","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningqiu","family":"Li","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhe","family":"Fu","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Key Laboratory of fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Liang, H., Zhang, L., Fu, X., Lin, Q., Liu, L., Niu, Y., Luo, X., Huang, Z., and Li, N. 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