{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:34:20Z","timestamp":1789011260206,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ISYPORT\u2014Integrated System for navigation risk mitigation in PORT","award":["CUP B64I20000330005"],"award-info":[{"award-number":["CUP B64I20000330005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Polychlorinated biphenyls (PCBs) are heterogeneous, synthetic, and widespread organochlorine compounds, and are one of the persistent organic pollutants present in improperly dumped waste and electronic equipment (e-waste), with a high bioaccumulation potential. In this study, the toxicity of Aroclor 1254 (a mixture of commercial PCBs) in human corneal epithelial cells (HCEpiCs), in an in vitro model of an ocular barrier, was evaluated. Aroclor 1254 (0.1\u201310 \u03bcg\/mL) reduced cell viability, trans-endothelial electric resistance (TEER) and cell migration. Moreover, it induced an inflammatory response, as indicated by the increase in cPLA2 activity, PGE2 production, phosphorylation of ERK 1\/2 and p-38, and release of inflammatory cytokines. Aroclor 1254 can damage corneal cells, compromising the integrity of the eye\u2019s outermost barrier. This damage may facilitate the occurrence of infectious processes that are physiologically prevented by the corneal barrier.<\/jats:p>","DOI":"10.3390\/ijms26030916","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T09:52:20Z","timestamp":1737539540000},"page":"916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Polychlorinated Biphenyls Induce Cytotoxicity and Inflammation in an In Vitro Model of an Ocular Barrier"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1709-5874","authenticated-orcid":false,"given":"Alessia","family":"Cosentino","sequence":"first","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8253-0598","authenticated-orcid":false,"given":"Aleksandra","family":"Agafonova","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9356-7289","authenticated-orcid":false,"given":"Luca","family":"Cavallaro","sequence":"additional","affiliation":[{"name":"Department of Civil Engineering and Architecture, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-0571","authenticated-orcid":false,"given":"Rosaria Ester","family":"Musumeci","sequence":"additional","affiliation":[{"name":"Department of Civil Engineering and Architecture, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9920-8310","authenticated-orcid":false,"given":"Chiara","family":"Prinzi","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinzia","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7541-656X","authenticated-orcid":false,"given":"Maria Teresa","family":"Cambria","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7951-1812","authenticated-orcid":false,"given":"Carmelina Daniela","family":"Anfuso","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9291-6969","authenticated-orcid":false,"given":"Gabriella","family":"Lupo","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1080\/10408449891344263","article-title":"Dioxin-like and Non-Dioxin-like Toxic Effects of Polychlorinated Biphenyls (PCBs): Implications for Risk Assessment","volume":"28","author":"Giesy","year":"1998","journal-title":"Crit. 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