{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T21:45:46Z","timestamp":1787262346223,"version":"build-2736575974"},"reference-count":32,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIAID\/NIH","award":["#U01AI129783"],"award-info":[{"award-number":["#U01AI129783"]}]},{"name":"NIAID\/NIH","award":["#5U54MD007592"],"award-info":[{"award-number":["#5U54MD007592"]}]},{"name":"National Institute on Minority Health and Health Disparities (NIMHD)","award":["#U01AI129783"],"award-info":[{"award-number":["#U01AI129783"]}]},{"name":"National Institute on Minority Health and Health Disparities (NIMHD)","award":["#5U54MD007592"],"award-info":[{"award-number":["#5U54MD007592"]}]},{"name":"scholarship from the COURI SURPASS program, jointly funded by the UTEP Vice President of Student Affairs (VPSA) office and the Campus Office of Undergraduate Research Initiatives (COURI)","award":["#U01AI129783"],"award-info":[{"award-number":["#U01AI129783"]}]},{"name":"scholarship from the COURI SURPASS program, jointly funded by the UTEP Vice President of Student Affairs (VPSA) office and the Campus Office of Undergraduate Research Initiatives (COURI)","award":["#5U54MD007592"],"award-info":[{"award-number":["#5U54MD007592"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chagas disease, caused by Trypanosoma cruzi, remains a significant global health challenge, particularly in the molecular diagnostics of low parasitemia during the chronic phase. This highlights the critical need for enhanced diagnostic methodologies. In response, this study evaluates the effectiveness of an automated magnetic beads-based DNA extraction method in improving the molecular diagnosis of Chagas disease compared to the traditional silica column-based extraction. Accordingly, this research seeks to enhance the DNA yield, purity, and sensitivity of real-time PCR (qPCR) assays for detecting T. cruzi satDNA. Blood samples spiked with guanidine\u2013EDTA solution and varying concentrations of T. cruzi were used to compare the two extraction methods. The results indicated that the magnetic bead-based method outperformed the silica column in terms of DNA concentration, purity, and earlier detection of T. cruzi satDNA. Although both methods had similar limits of detection at a 95% confidence interval, the magnetic bead-based approach demonstrated higher sensitivity and reproducibility, particularly in low-parasitemia samples. The findings suggest that the magnetic beads-based DNA extraction method offers a more reliable, faster, and more sensitive alternative for diagnosing chronic Chagas disease, potentially improving clinical outcomes by enabling more accurate and earlier parasite detection.<\/jats:p>","DOI":"10.3390\/ijms26030937","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T09:01:13Z","timestamp":1737622873000},"page":"937","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Utility of Magnetic Bead-Based Automated DNA Extraction to Improve Chagas Disease Molecular Diagnosis"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1680-1027","authenticated-orcid":false,"given":"Priscila S. G.","family":"Farani","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7600-1906","authenticated-orcid":false,"given":"Amanda","family":"Faier-Pereira","sequence":"additional","affiliation":[{"name":"Platform of Molecular Analysis, Laboratory of Molecular Virology and Parasitology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5430-7222","authenticated-orcid":false,"given":"Alejandro Marcel","family":"Hasslocher-Moreno","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor C.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-8697","authenticated-orcid":false,"given":"Otacilio C.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Platform of Molecular Analysis, Laboratory of Molecular Virology and Parasitology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,23]]},"reference":[{"key":"ref_1","first-page":"351","article-title":"\u00dcber eine neue Trypanosomiasis des Menschen","volume":"Volume 13","author":"Chagas","year":"1909","journal-title":"Archiv f\u00fcr Schiffs und Tropen Hygiene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"ITC17","DOI":"10.7326\/AITC202302210","article-title":"Chagas Disease","volume":"176","author":"Hochberg","year":"2023","journal-title":"Ann. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0140-6736(17)31612-4","article-title":"Chagas disease","volume":"391","author":"Molina","year":"2018","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.actatropica.2009.11.003","article-title":"Chagas disease: A Latin American health problem becoming a world health problem","volume":"115","author":"Schmunis","year":"2010","journal-title":"Acta Trop."},{"key":"ref_5","unstructured":"Centers for Disease Control and Prevention (CDC) (2024, August 15). Chagas Disease, Available online: https:\/\/www.cdc.gov\/chagas\/index.html."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1128\/JCM.00142-16","article-title":"Serological Diagnosis of Chronic Chagas Disease: Is It Time for a Change?","volume":"54","author":"Abras","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez, C., Cucunub\u00e1, Z., Fl\u00f3rez, C., Olivera, M., Valencia, C., Zambrano, P., Le\u00f3n, C., and Ram\u00edrez, J.D. (2016). Molecular Diagnosis of Chagas Disease in Colombia: Parasitic Loads and Discrete Typing Units in Patients from Acute and Chronic Phases. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004997"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.actatropica.2018.02.019","article-title":"Molecular diagnosis of Trypanosoma cruzi","volume":"184","author":"Schijman","year":"2018","journal-title":"Acta Trop."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e200444","DOI":"10.1590\/0074-02760200444","article-title":"Parasitological, serological and molecular diagnosis of acute and chronic Chagas disease: From field to laboratory","volume":"117","author":"Schijman","year":"2022","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.actatropica.2012.08.020","article-title":"Towards the establishment of a consensus real-time qPCR to monitor Trypanosoma cruzi parasitemia in patients with chronic Chagas disease cardiomyopathy: A substudy from the BENEFIT trial","volume":"125","author":"Moreira","year":"2013","journal-title":"Acta Trop."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1186\/s13071-015-0770-0","article-title":"Usefulness of real time PCR to quantify parasite load in serum samples from chronic Chagas disease patients","volume":"8","author":"Melo","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.jmoldx.2015.04.010","article-title":"Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients","volume":"17","author":"Ramirez","year":"2015","journal-title":"J. Mol. Diagn."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s40249-023-01143-7","article-title":"Molecular diagnosis of Chagas disease: A systematic review and meta-analysis","volume":"12","author":"Muro","year":"2023","journal-title":"Infect. Dis. Poverty"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e711","DOI":"10.1016\/S2666-5247(21)00265-2","article-title":"Pathogen diversity, immunity, and the fate of infections: Lessons learned from Trypanosoma cruzi human-host interactions","volume":"3","author":"Gollob","year":"2022","journal-title":"Lancet Microbe"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1056\/NEJMoa1507574","article-title":"Randomized Trial of Benznidazole for Chronic Chagas\u2019 Cardiomyopathy","volume":"373","author":"Morillo","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mule, S.N., Costa-Martins, A.G., Rosa-Fernandes, L., de Oliveira, G.S., Rodrigues, C.M.F., Quina, D., Rosein, G.E., Teixeira, M.M.G., and Palmisano, G. (2021). PhyloQuant approach provides insights into Trypanosoma cruzi evolution using a systems-wide mass spectrometry-based quantitative protein profile. Commun. Biol., 4.","DOI":"10.1038\/s42003-021-01762-6"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e052897","DOI":"10.1136\/bmjopen-2021-052897","article-title":"New chemotherapy regimens and biomarkers for Chagas disease: The rationale and design of the TESEO study, an open-label, randomised, prospective, phase-2 clinical trial in the Plurinational State of Bolivia","volume":"11","author":"Urbina","year":"2021","journal-title":"BMJ Open"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/S1473-3099(23)00629-1","article-title":"Efficacy of three benznidazole dosing strategies for adults living with chronic Chagas disease (MULTIBENZ): An international, randomised, double-blind, phase 2b trial","volume":"24","author":"Sulleiro","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1186\/s13063-020-4226-2","article-title":"Efficacy and safety assessment of different dosage of benznidazol for the treatment of Chagas disease in chronic phase in adults (MULTIBENZ study): Study protocol for a multicenter randomized Phase II non-inferiority clinical trial","volume":"21","author":"Fernandez","year":"2020","journal-title":"Trials"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Marchiol, A., Florez Sanchez, A.C., Caicedo, A., Segura, M., Bautista, J., Ayala Sotelo, M.S., Herazo, R., Forsyth, C., and Bohorquez, L.C. (2023). Laboratory evaluation of eleven rapid diagnostic tests for serological diagnosis of Chagas disease in Colombia. PLoS Neglected Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011547"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Moreira, O.C., Fernandes, A.G., Gomes, N., Dos Santos, C.M., Jacomasso, T., Costa, A.D.T., Nascimento, L.O.R., Hasslocher-Moreno, A.M., do Brasil, P., and Morello, L.G. (2023). Validation of the NAT Chagas IVD Kit for the Detection and Quantification of Trypanosoma cruzi in Blood Samples of Patients with Chagas Disease. Life, 13.","DOI":"10.3390\/life13061236"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1080\/14737159.2017.1338566","article-title":"Molecular diagnostics for Chagas disease: Up to date and novel methodologies","volume":"17","author":"Gallego","year":"2017","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.actatropica.2007.05.019","article-title":"Development of a real-time PCR assay for Trypanosoma cruzi detection in blood samples","volume":"103","author":"Piron","year":"2007","journal-title":"Acta Trop."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1128\/CMR.00074-09","article-title":"Validation of laboratory-developed molecular assays for infectious diseases","volume":"23","author":"Burd","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1080\/14737159.2019.1659729","article-title":"The use of qPCR in human Chagas disease: A systematic review","volume":"19","author":"Marques","year":"2019","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.2217\/fmb-2020-0023","article-title":"Comparison of DNA extraction methods and real-time PCR assays for the molecular diagnostics of chronic Chagas disease","volume":"15","author":"Silgado","year":"2020","journal-title":"Future Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Parrado, R., Ramirez, J.C., de la Barra, A., Alonso-Vega, C., Juiz, N., Ortiz, L., Illanes, D., Torrico, F., Gascon, J., and Alves, F. (2019). Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease. Antimicrob. Agents Chemother., 63.","DOI":"10.1128\/AAC.01191-18"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/j.ijpara.2007.04.015","article-title":"Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease","volume":"37","author":"Burgos","year":"2007","journal-title":"Int. J. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.jmoldx.2020.12.007","article-title":"Development and Evaluation of a Three-Dimensional Printer\u2014Based DNA Extraction Method Coupled to Loop Mediated Isothermal Amplification for Point-of-Care Diagnosis of Congenital Chagas Disease in Endemic Regions","volume":"23","author":"Wehrendt","year":"2021","journal-title":"J. Mol. Diagn."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Rodrigues-dos-Santos, \u00cd., Melo, M.F., de Castro, L., Hasslocher-Moreno, A.M., do Brasil, P.E.A.A., Silvestre de Sousa, A., Britto, C., and Moreira, O.C. (2018). Exploring the parasite load and molecular diversity of Trypanosoma cruzi in patients with chronic Chagas disease from different regions of Brazil. PLoS Neglected Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006939"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e00402021","DOI":"10.1590\/0037-8682-0040-2021","article-title":"Temporal changes in the clinical-epidemiological profile of patients with Chagas disease at a referral center in Brazil","volume":"54","author":"Saraiva","year":"2021","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"World Medical Association (2013). World Medical Association Declaration of Helsinki\u2014Ethical Principles for Medical Research Involving Human Subjects. JAMA, 310, 2191\u20132194.","DOI":"10.1001\/jama.2013.281053"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/3\/937\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:34:18Z","timestamp":1759919658000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/3\/937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,23]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["ijms26030937"],"URL":"https:\/\/doi.org\/10.3390\/ijms26030937","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,23]]}}}