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To establish a clinically relevant model and explore the mechanisms underlying NK pathogenesis, we developed a novel mouse model through partial transection of the ciliary nerve. This approach mimics the progressive nature of NK, reproducing key clinical features such as corneal epithelial defects, reduced sensitivity, diminished tear secretion, and delayed wound healing. Using this model, we investigated how disruptions in mitochondrial dynamics contribute to corneal epithelial dysfunction and impaired repair in NK. Our findings revealed substantial disruptions in mitochondrial dynamics, including reduced expression of fusion proteins (OPA1), downregulation of fission regulators (FIS1 and MFF), and impaired mitochondrial transport, as evidenced by decreased expression of Rhot1 and Kif5b. Additionally, the downregulation of mitophagy-related genes (Pink1 and Prkn) contributed to the accumulation of dysfunctional mitochondria, leading to DNA damage and impaired corneal epithelial repair. These mitochondrial abnormalities were accompanied by increased \u03b3H2AX staining, indicative of DNA double-strand breaks and cellular stress. This study highlights the pivotal role of mitochondrial dynamics in corneal epithelial health and repair, suggesting that therapeutic strategies aimed at restoring mitochondrial function, enhancing mitophagy, and mitigating oxidative stress may offer promising avenues for treating NK.<\/jats:p>","DOI":"10.3390\/ijms26031290","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T04:36:51Z","timestamp":1738557411000},"page":"1290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Disrupted Mitochondrial Dynamics Impair Corneal Epithelial Healing in Neurotrophic Keratopathy"],"prefix":"10.3390","volume":"26","author":[{"given":"Mengyi","family":"Jin","sequence":"first","affiliation":[{"name":"Hengyang Medical School, University of South China, Hengyang 421001, 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Cataract. Refract. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1080\/07853890.2022.2045035","article-title":"Neurotrophic keratopathy: Current challenges and future prospects","volume":"54","author":"NaPier","year":"2022","journal-title":"Ann. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1002\/jcp.25623","article-title":"Understanding the Pathogenesis of Neurotrophic Keratitis: The Role of Corneal Nerves","volume":"232","author":"Mastropasqua","year":"2017","journal-title":"J. Cell. Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jtos.2019.11.008","article-title":"Neurotrophic keratitis: Frequency, etiologies, clinical management and outcomes","volume":"18","author":"Saad","year":"2020","journal-title":"Ocul. Surf."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4670","DOI":"10.1167\/iovs.17-22159","article-title":"Colony Stimulating Factor-1 Receptor Expressing Cells Infiltrating the Cornea Control Corneal Nerve Degeneration in Response to HSV-1 Infection","volume":"58","author":"Gurung","year":"2017","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2665","DOI":"10.1016\/j.ajpath.2016.06.007","article-title":"IL-6 Contributes to Corneal Nerve Degeneration after Herpes Simplex Virus Type I Infection","volume":"186","author":"Jinkins","year":"2016","journal-title":"Am. J. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1016\/S0161-6420(00)00163-9","article-title":"Topical treatment with nerve growth factor for neurotrophic keratitis","volume":"107","author":"Bonini","year":"2000","journal-title":"Ophthalmology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nrm4039","article-title":"The molecular era of the mitochondrial calcium uniporter","volume":"16","author":"Kamer","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1038\/s41556-024-01429-4","article-title":"Mitochondria and cell death","volume":"26","author":"Glover","year":"2024","journal-title":"Nat. Cell Biol. Nat. Cell Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.jtbi.2006.07.019","article-title":"A simplified model for mitochondrial ATP production","volume":"243","author":"Bertram","year":"2006","journal-title":"J. Theor. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/0891-5849(95)00037-X","article-title":"Mitochondrial superoxide and hydrogen peroxide generation, protein oxidative damage, and longevity in different species of flies","volume":"19","author":"Sohal","year":"1995","journal-title":"Free Radic. Biol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1146\/annurev-pathmechdis-012419-032711","article-title":"Mitochondrial Dynamics and Its Involvement in Disease","volume":"15","author":"Chan","year":"2020","journal-title":"Annu. Rev. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.cmet.2014.03.011","article-title":"Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation","volume":"19","author":"Mishra","year":"2014","journal-title":"Cell Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/nature21077","article-title":"MFN1 structures reveal nucleotide-triggered dimerization critical for mitochondrial fusion","volume":"542","author":"Cao","year":"2017","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1083\/jcb.201609019","article-title":"Structures of human mitofusin 1 provide insight into mitochondrial tethering","volume":"215","author":"Qi","year":"2016","journal-title":"J. Cell Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s00018-017-2603-0","article-title":"The ever-growing complexity of the mitochondrial fission machinery","volume":"75","author":"Pagliuso","year":"2018","journal-title":"Cell Mol. Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1091\/mbc.e12-10-0721","article-title":"Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission","volume":"24","author":"Loson","year":"2013","journal-title":"Mol. Biol. Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1016\/j.jgg.2024.09.009","article-title":"Variants within KIF5B are associated with weight loss through mitochondrial transport alteration in sheep","volume":"51","author":"Chen","year":"2024","journal-title":"J. Genet. Genom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"102000","DOI":"10.1016\/j.mito.2024.102000","article-title":"Mitochondria-targeted nanotherapeutics: A new Frontier in neurodegenerative disease treatment","volume":"81","author":"Keethedeth","year":"2024","journal-title":"Mitochondrion"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nrneurol.2014.228","article-title":"Disturbed mitochondrial dynamics and neurodegenerative disorders","volume":"11","author":"Burte","year":"2015","journal-title":"Nat. Rev. Neurol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/j.bbadis.2016.11.010","article-title":"Mitochondrial dysfunction and oxidative stress in metabolic disorders\u2014A step towards mitochondria based therapeutic strategies","volume":"1863","author":"Bhatti","year":"2017","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1113\/JP271301","article-title":"Mitochondrial dynamics, mitophagy and cardiovascular disease","volume":"594","author":"Pennanen","year":"2016","journal-title":"J. Physiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/BF02150440","article-title":"A technique for controlled sensory denervation of the rabbit cornea","volume":"218","author":"Schimmelpfennig","year":"1982","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2532","DOI":"10.1167\/iovs.10-5688","article-title":"A novel mouse model for neurotrophic keratopathy: Trigeminal nerve stereotactic electrolysis through the brain","volume":"52","author":"Ferrari","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.preteyeres.2018.04.003","article-title":"Neurotrophic keratopathy","volume":"66","author":"Dua","year":"2018","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Yamaguchi, T., Turhan, A., Harris, D.L., Hu, K., Pruss, H., von Andrian, U., and Hamrah, P. (2013). Bilateral nerve alterations in a unilateral experimental neurotrophic keratopathy model: A lateral conjunctival approach for trigeminal axotomy. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0070908"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0014-4835(03)00050-2","article-title":"Corneal nerves: Structure, contents and function","volume":"76","author":"Muller","year":"2003","journal-title":"Exp. Eye Res."},{"key":"ref_30","first-page":"103","article-title":"Localization of neuropeptides in the cornea and uvea of the rat: An immunohistochemical study","volume":"28","author":"Shimizu","year":"1982","journal-title":"Cell Mol. Biol."},{"key":"ref_31","first-page":"671","article-title":"Substance P-immunoreactive nerves in the human cornea and iris","volume":"23","author":"Tervo","year":"1982","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1167\/iovs.03-0189","article-title":"Effects of substance P and IGF-1 in corneal epithelial barrier function and wound healing in a rat model of neurotrophic keratopathy","volume":"44","author":"Nagano","year":"2003","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.exer.2014.05.006","article-title":"Corneal epithelial and neuronal interactions: Role in wound healing","volume":"125","author":"Kowtharapu","year":"2014","journal-title":"Exp. Eye Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1002\/jcb.240520411","article-title":"Stimulation of epithelial cell growth by the neuropeptide substance P","volume":"52","author":"Reid","year":"1993","journal-title":"J. Cell Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/0196-9781(89)90182-4","article-title":"Substance P and mitochondrial oxygen consumption: Evidence for a direct intracellular role for the peptide","volume":"10","author":"Prabhakar","year":"1989","journal-title":"Peptides"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.biopha.2017.03.043","article-title":"Cardioprotective effects of calcitonin gene-related peptide in isolated rat heart and in H9c2 cells via redox signaling","volume":"90","author":"Tullio","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1161\/ATVBAHA.115.305469","article-title":"Reduction of Mitochondria-Endoplasmic Reticulum Interactions by Acetylcholine Protects Human Umbilical Vein Endothelial Cells From Hypoxia\/Reoxygenation Injury","volume":"35","author":"He","year":"2015","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"107154","DOI":"10.1016\/j.phrs.2024.107154","article-title":"Serotonin regulation of mitochondria in kidney diseases","volume":"203","author":"Hurtado","year":"2024","journal-title":"Pharmacol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/nrm1697","article-title":"Mitochondrial fission in apoptosis","volume":"6","author":"Youle","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1167\/iovs.65.6.14","article-title":"Mitochondria Transplantation Promotes Corneal Epithelial Wound Healing","volume":"65","author":"Raz","year":"2024","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"L1005","DOI":"10.1152\/ajplung.2000.279.6.L1005","article-title":"Reactive oxygen species in cell signaling","volume":"279","author":"Thannickal","year":"2000","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.freeradbiomed.2022.09.032","article-title":"Altered glucose metabolism in Alzheimer\u2019s disease: Role of mitochondrial dysfunction and oxidative stress","volume":"193","author":"Dewanjee","year":"2022","journal-title":"Free Radic. Biol. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1042\/EBC20190007","article-title":"Nuclear failure, DNA damage, and cell cycle disruption after migration through small pores: A brief review","volume":"63","author":"Pfeifer","year":"2019","journal-title":"Essays Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"101039","DOI":"10.1016\/j.preteyeres.2021.101039","article-title":"The impact of sensory neuropathy and inflammation on epithelial wound healing in diabetic corneas","volume":"89","author":"Yu","year":"2022","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.jtos.2020.09.007","article-title":"The molecular basis of neurotrophic keratopathy: Diagnostic and therapeutic implications. A review","volume":"19","year":"2021","journal-title":"Ocul. Surf."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Zhang, L., An, H., Zhen, R., Zhang, T., Ding, M., Zhang, M., Sun, Y., and Gu, C. (2025). Di Huang Yi Zhi Fang improves cognitive function in APP\/PS1 mice by inducing neuronal mitochondrial autophagy through the PINK1-parkin pathway. J. Alzheimers Dis., 13872877241299832.","DOI":"10.1177\/13872877241299832"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yashooa, R.K., Duranti, E., Conconi, D., Lavitrano, M., Mustafa, S.A., and Villa, C. (2025). Mitochondrial microRNAs: Key Drivers in Unraveling Neurodegenerative Diseases. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26020626"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0006-8993(78)90533-4","article-title":"The neuronal pathway subserving the pupillary light reflex","volume":"159","author":"Hultborn","year":"1978","journal-title":"Brain Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4109","DOI":"10.1167\/iovs.13-12075","article-title":"Neuroprotectin D1 restores corneal nerve integrity and function after damage from experimental surgery","volume":"54","author":"Cortina","year":"2013","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1167\/iovs.14-15596","article-title":"Degeneration and regeneration of corneal nerves in response to HSV-1 infection","volume":"56","author":"Zheng","year":"2015","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1167\/iovs.63.3.3","article-title":"Obstructive Sleep Apnea Affects Lacrimal Gland Function","volume":"63","author":"Wang","year":"2022","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jtos.2023.12.002","article-title":"A PEDF peptide mimetic effectively relieves dry eye in a diabetic murine model by restoring corneal nerve, barrier, and lacrimal gland function","volume":"32","author":"Chen","year":"2024","journal-title":"Ocul. Surf."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5813","DOI":"10.1167\/iovs.13-13546","article-title":"Serine protease inhibitor A3K suppressed the formation of ocular surface squamous metaplasia in a mouse model of experimental dry eye","volume":"55","author":"Lin","year":"2014","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/0014-4886(80)90154-5","article-title":"Sensory denervation of the rabbit cornea affects epithelial properties","volume":"69","author":"Beuerman","year":"1980","journal-title":"Exp. Neurol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1097\/00055735-199908000-00009","article-title":"Management of neurotrophic keratopathy","volume":"10","author":"Lambiase","year":"1999","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5194","DOI":"10.1167\/iovs.18-24803","article-title":"Effect of Human Corneal Mesenchymal Stromal Cell-derived Exosomes on Corneal Epithelial Wound Healing","volume":"59","author":"Samaeekia","year":"2018","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1167\/iovs.63.12.14","article-title":"Sirt1 Regulates Corneal Epithelial Migration by Deacetylating Cortactin","volume":"63","author":"Lin","year":"2022","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1186\/s12974-020-01812-6","article-title":"The neuroregenerative effects of topical decorin on the injured mouse cornea","volume":"17","author":"Wu","year":"2020","journal-title":"J. Neuroinflamm."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.exer.2018.03.012","article-title":"Recovery of the sub-basal nerve plexus and superficial nerve terminals after corneal epithelial injury in mice","volume":"171","author":"Downie","year":"2018","journal-title":"Exp. Eye Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"118685","DOI":"10.1016\/j.jep.2024.118685","article-title":"Jin-Xin-Kang alleviates heart failure by mitigating mitochondrial dysfunction through the Calcineurin\/Dynamin-Related Protein 1 signaling pathway","volume":"335","author":"Lin","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0167-4889(02)00259-8","article-title":"Functions of outer membrane receptors in mitochondrial protein import","volume":"1592","author":"Endo","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"E87","DOI":"10.1152\/ajpendo.00457.2019","article-title":"A pipeline for multidimensional confocal analysis of mitochondrial morphology, function, and dynamics in pancreatic beta-cells","volume":"318","author":"Chaudhry","year":"2020","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12964-020-0529-x","article-title":"Knocking-out the Siah2 E3 ubiquitin ligase prevents mitochondrial NCX3 degradation, regulates mitochondrial fission and fusion, and restores mitochondrial function in hypoxic neurons","volume":"18","author":"Sisalli","year":"2020","journal-title":"Cell Commun. 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