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Silica (SiO2) serves as a prominent support material for enzyme immobilization. Recent advancements have led to the development of various silica-binding proteins (SBPs) and silica-forming proteins (SFPs) that are invaluable tools in immobilizing enzymes on siliceous materials in a fast and simple manner. SBPs facilitate the immobilization of enzymes with controlled orientation on silica surfaces, while SFPs enable the biomimetic synthesis and encapsulation of enzymes within silica particles. In this review, we explore recent advances in the use of SBPs and SFPs in enzyme applications. We provide a comprehensive overview of their mechanisms and sequence characteristics relevant to enzyme immobilization. Additionally, we summarize the recombinant production and immobilization procedures for enzymes with SBPs or SFPs. We then categorize the available SBPs and SFPs into naturally occurring and artificially engineered types, presenting recent examples that demonstrate their utilization in enzyme immobilization. Our review highlights the strengths and limitations of various SBPs and SFPs and sheds light on future directions for the development of tailor-made biocatalytic silica.<\/jats:p>","DOI":"10.3390\/ijms26031304","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T12:18:56Z","timestamp":1738585136000},"page":"1304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Exploiting Silica-Binding and Silica-Forming Proteins as Versatile Tools for One-Step Enzyme Immobilization on Siliceous Materials"],"prefix":"10.3390","volume":"26","author":[{"given":"Gyun Taek","family":"Lim","sequence":"first","affiliation":[{"name":"Division of Applied Life Science (BK21 Four) and Anti-Aging Bio Cell Factory Regional Leading Research Center (ABC-RLRC), Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6408-4317","authenticated-orcid":false,"given":"Byung Hoon","family":"Jo","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 Four) and Anti-Aging Bio Cell Factory Regional Leading Research Center (ABC-RLRC), Gyeongsang National University, Jinju 52828, Republic of Korea"},{"name":"Division of Life Science and Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5184","DOI":"10.1021\/acsomega.2c07560","article-title":"Enzyme immobilization technologies and industrial applications","volume":"8","author":"Maghraby","year":"2023","journal-title":"ACS Omega"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Cavalcante, F.T.T., Cavalcante, A.L.G., de Sousa, I.G., Neto, F.S., and dos Santos, J.C.S. 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