{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T17:06:09Z","timestamp":1761930369160,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D program of China","doi-asserted-by":"publisher","award":["2023YFD1801100","LBH-Z23085"],"award-info":[{"award-number":["2023YFD1801100","LBH-Z23085"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"program for Postdoctoral of Heilongjiang Province","award":["2023YFD1801100","LBH-Z23085"],"award-info":[{"award-number":["2023YFD1801100","LBH-Z23085"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Trueperella pyogenes is a widely distributed opportunistic pathogenic bacterium that can infect livestock, wildlife, community animals, and humans, resulting in suppurative infection of tissue and organ mucosa, including pneumonia, liver abscessation, mastitis, metritis, endocarditis, and osteoarthritis. TP1804 and TP1808 were isolated from the uterine lavage fluid of cows with endometritis. This study analyzed the prokaryotic transcriptomics and proteomics of two strains of T. pyogenes with similar growth curves but different cytotoxicity. Studying the metabolic mechanisms of these differentially expressed genes and proteins can greatly promote the discovery of new biomarkers and improve the accuracy of biomarker identification, which is of great value for molecular mechanisms, biomarkers, early diagnosis of diseases, molecular typing, and prognosis. Our results indicate that the control of the virulence by tRNAs to bacteria during ribosome biosynthesis is crucial.<\/jats:p>","DOI":"10.3390\/ijms26041490","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T05:34:32Z","timestamp":1739252072000},"page":"1490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Combined Prokaryotic Transcriptomics and Proteomics Analysis of Clinical Trueperella pyogenes Isolates with Distinctive Cytotoxicity"],"prefix":"10.3390","volume":"26","author":[{"given":"Ning","family":"Liu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"},{"name":"Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Liang, Y., Yu, L., Chen, M., Guo, Y., Kang, Z., Qu, C., Tian, C., Zhang, D., and Liu, M. 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