{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:57:30Z","timestamp":1786431450537,"version":"build-2736575974"},"reference-count":125,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Funds for the Central Universities","award":["2572022BD03"],"award-info":[{"award-number":["2572022BD03"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["31900064"],"award-info":[{"award-number":["31900064"]}]},{"name":"National Natural Science Foundation of China","award":["2572022BD03"],"award-info":[{"award-number":["2572022BD03"]}]},{"name":"National Natural Science Foundation of China","award":["31900064"],"award-info":[{"award-number":["31900064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Methylxanthines are ubiquitous purine alkaloids in nature and have rich biological activities and functions. Today, the demand for methylxanthine is increasing but its production is low. This issue prevents its widespread use in many industrial fields, such as pharmaceuticals, food manufacturing, and chemical engineering. To address these issues, this review provides a comprehensive and systematic exploration of methylxanthines, delving into their biological structures, detailed biosynthetic pathways, and the latest research trends. These findings serve as valuable references for researchers, fostering advancements in the optimization of synthesis processes for methylxanthines and their derivatives and promoting their application across diverse industrial fields, such as medicine, food, and chemical engineering. By bridging fundamental research and practical applications, this work aims to advance the understanding of methylxanthine compounds, enhance their production efficiency, and contribute to healthcare and technological progress.<\/jats:p>","DOI":"10.3390\/ijms26041510","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T09:36:05Z","timestamp":1739266565000},"page":"1510","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Progress in Methylxanthine Biosynthesis: Insights into Pathways and Engineering Strategies"],"prefix":"10.3390","volume":"26","author":[{"given":"Tongtong","family":"Jiang","sequence":"first","affiliation":[{"name":"School of Life Science, Northeast Forestry University, Harbin 150040, China"},{"name":"Key Laboratory for Enzyme and Enzyme-Like Material Engineering of Heilongjiang, College of Life Science, Northeast Forestry University, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangci","family":"Zuo","sequence":"additional","affiliation":[{"name":"School of Life Science, Northeast Forestry University, Harbin 150040, China"},{"name":"Key Laboratory for Enzyme and Enzyme-Like Material Engineering of Heilongjiang, College of Life Science, Northeast Forestry University, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0500-2821","authenticated-orcid":false,"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Science, Northeast Forestry University, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanbin","family":"Xing","sequence":"additional","affiliation":[{"name":"Aulin College, Northeast Forestry University, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8268-3500","authenticated-orcid":false,"given":"Pengchao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science, Northeast Forestry University, Harbin 150040, China"},{"name":"Key Laboratory for Enzyme and Enzyme-Like Material Engineering of Heilongjiang, College of Life Science, Northeast Forestry University, Harbin 150040, China"},{"name":"Aulin College, Northeast Forestry University, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1467-789X.2007.00389.x","article-title":"A short history of beverages and how our body treats them","volume":"9","author":"Wolf","year":"2007","journal-title":"Obes. 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