{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:43:20Z","timestamp":1787697800374,"version":"build-2784847793"},"reference-count":52,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pisa University through Fondi di Ateneo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brucellosis is a zoonosis that affects domestic and wild animals, causing reproductive disorders and significant economic losses in livestock. Brucella melitensis, B. abortus, and B. suis are the main agents of brucellosis in livestock and humans, thereby their control and eradication are crucial. Serological tests based on identification of antibodies against Brucella smooth lipopolysaccharides (sLPS) in the serum of infected animals are traditionally used. This approach shows two main limits: (i) tests can give false positives due to the similarity of Brucella sLPS with the LPS of other Gram-negative bacteria; (ii) antigen production represents a possible risk of zoonoses. In this work, a proteomic approach, starting from B. melitensis Brucellergene, was employed to identify possible Brucella antigenic proteins useful for a more specific and safe serological diagnosis. Four proteins binding to the infected swine serum were identified: (i) \u201cprobable sugar-binding periplasmic protein B. abortus str 2308A\u201d; (ii) \u201cpeptide ABC transporter substrate-binding protein B. melitensis\u201d; (iii) \u201cGntR family transcriptional regulator B. melitensis\u201d; (iv) \u201cconserved hypothetical protein B. melitensis M28\u201d. These proteins could be promising specific antigens for serological investigations in swine. In the near future, these antigenic proteins could be synthesized in vitro and used to produce a safer and more specific diagnostic kit.<\/jats:p>","DOI":"10.3390\/ijms26041517","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T09:36:05Z","timestamp":1739266565000},"page":"1517","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preliminary Investigation Towards a Safety Tool for Swine Brucellosis Diagnosis by a Proteomic Approach Within the One-Health Framework"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1592-7522","authenticated-orcid":false,"given":"Simona","family":"Sagona","sequence":"first","affiliation":[{"name":"Department of Veterinary Science, Pisa University, viale delle Piagge 2, 56124 Pisa, Italy"},{"name":"Department of Pharmacy, Pisa University, via Bonanno 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-0613","authenticated-orcid":false,"given":"Fabrizio","family":"Bertelloni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Pisa University, viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Turchi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Pisa University, viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0114-5872","authenticated-orcid":false,"given":"Paola","family":"Roncada","sequence":"additional","affiliation":[{"name":"Department of Health Science, University \u201cMagna Graecia\u201d of Catanzaro, viale Europa, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1672-8181","authenticated-orcid":false,"given":"Elena","family":"Tafi","sequence":"additional","affiliation":[{"name":"CREA Research Centre for Agriculture and Environment, via di Corticella 133, 40128 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1717-2947","authenticated-orcid":false,"given":"Filippo","family":"Fratini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Pisa University, viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0808-3037","authenticated-orcid":false,"given":"Antonio","family":"Felicioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Pisa University, viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Cerri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Pisa University, viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2174\/1874318801004010109","article-title":"Pathogenesis of Brucella spp.","volume":"4","author":"Xavier","year":"2010","journal-title":"Open Vet. 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